• 1 Universiti Kebangsaan Malaysia


Development of insects in laboratory for minimum post mortem interval estimation (mPMI) or time of colonisation (TOC) in
forensic entomology can be affected by the type and quality of food consumed during larval period. Since mPMI estimation
also involves analysis of larval specimens collected from burned human remains, it is important to study if burned tissues
could affect growth of sarcosaprophagous larvae. This study investigated the effect of burned tissues on the size and
developmental period of Megaselia scalaris (Loew) (Diptera: Phoridae), a species of forensic importance. Development
of M. scalaris on 75 g burned cow’s liver was compared with control liver in three study replicates. Mean larval length
(2.87 ± 0.11 mm) and weight (0.81 ± 0.08 mg) of M. scalaris larvae in burned liver diets were significantly lower than
larval length (5.03 ± 0.15 mm) and weight (2.85 ± 0.21 mg) of control liver diets (p < 0.001) whilst mean pupal length
(2.53 ± 0.06 mm) and weight (0.92 ± 0.06 mg) in burned liver diets were significantly lower than pupal length (3.52 ±
0.06 mm) and weight (2.84 ± 0.16 mg) in control liver diets (p < 0.001). Development of larvae in burned liver was 5-9
hours slower than those feeding on control liver based on single observation. Although the assessment is preliminary, the
findings indicate physical growth of larvae feeding on burned animal tissues was affected and entomological specimens
recovered from burned remains should be evaluated carefully to avoid errors in mPMI/TOC estimation. Limitations and
suggestions for further research are also presented herein.