Whole genome sequence of moderate halophilic marine bacterium Marinobacter litoralis SW-45: Abundance of non-coding RNAs

Musa H 1 , Kasim FH 2 , Gunny AAN 3 , Gopinath SCB 4 , Chinni SV 5 , Ahmad MA 6

Affiliations 

  • 1 School of Bioprocess Engineering, Universiti Malaysia Perlis UNIMAP, Kompleks Pusat Pengajian Jejawi 3, Arau, Perlis, 02600, Malaysia; Centre of Excellence for Biomass Utilization, School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, Arau, Perlis, 02600 Malaysia. Electronic address: hallyruh@gmail.com
  • 2 School of Bioprocess Engineering, Universiti Malaysia Perlis UNIMAP, Kompleks Pusat Pengajian Jejawi 3, Arau, Perlis, 02600, Malaysia; Centre of Excellence for Biomass Utilization, School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, Arau, Perlis, 02600 Malaysia. Electronic address: farizul@unimap.edu.my
  • 3 Centre of Excellence for Biomass Utilization, School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, Arau, Perlis, 02600 Malaysia; Department of Chemical Engineering Technology, Faculty of Engineering Technology, Universiti Malaysia Perlis, Kampus UniCITI Alam, Sungai Chuchuh, Padang Besar 02100, Perlis, Malaysia. Electronic address: ahmadanas@unimap.edu.my
  • 4 School of Bioprocess Engineering, Universiti Malaysia Perlis UNIMAP, Kompleks Pusat Pengajian Jejawi 3, Arau, Perlis, 02600, Malaysia. Electronic address: subash@unimap.edu.my
  • 5 Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong, 08100, Malaysia. Electronic address: v_suresh@aimst.edu.my
  • 6 School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, Seri Ampangan, Nibong Tebai, Penang, 14300, Malaysia. Electronic address: chazmier@eng.usm.my
Int J Biol Macromol, 2019 Jul 15;133:1288-1298.
PMID: 31055112 DOI: 10.1016/j.ijbiomac.2019.05.003

Abstract

A report on the de novo Whole Genome Sequence (WGS) of Marinobacter litoralis SW-45, a moderately salt-tolerant bacterium isolated from the seawater in Malaysia is presented. The strain has a genome size of 3.45 Mb and is capable of producing halophilic lipase, protease and esterase enzymes. Computational prediction of non-coding RNA (ncRNA) genes in M. litoralis SW-45 was performed using standalone software known as the non-coding RNA characterization (nocoRNAc). In addition, a phylogenetic tree showing the evolutionary relationship between the strain and other members of the genus Marinobacter was constructed using 16SrRNA sequence information. A total of 385 ncRNA transcripts, 1124 terminator region, and 2350 Stress Induced Duplex Destabilization sites were predicted. The current WGS shotgun project has provided the relevant genetic information that may be useful for the strain's improvement studies. This manuscript gives the first description of M. litoralis with a complete genome.

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