Potential halophilic cellulases for in situ enzymatic saccharification of ionic liquids pretreated lignocelluloses

Gunny AA 1 , Arbain D 2 , Edwin Gumba R 1 , Jong BC 3 , Jamal P 4

Affiliations 

  • 1 School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia
  • 2 School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia. Electronic address: dachyar@unimap.edu.my
  • 3 Agrotechnology and Biosciences Division, Malaysian Nuclear Agency (Nuclear Malaysia), Ministry of Science, Technology and Innovation, Bangi, 43000 Kajang, Selangor, Malaysia
  • 4 Bioenvironmental Engineering Research Center (BERC), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, 50728 Gombak, Kuala Lumpur, Malaysia
Bioresour Technol, 2014 Mar;155:177-81.
PMID: 24457303 DOI: 10.1016/j.biortech.2013.12.101

Abstract

Ionic liquids (ILs) have been used as an alternative green solvent for lignocelluloses pretreatment. However, being a salt, ILs exhibit an inhibitory effect on cellulases activity, thus making the subsequent saccharification inefficient. The aim of the present study is to produce salt-tolerant cellulases, with the rationale that the enzyme also tolerant to the presence of ILs. The enzyme was produced from a locally isolated halophilic strain and was characterized and assessed for its tolerance to different types of ionic liquids. The results showed that halophilic cellulases produced from Aspergillus terreus UniMAP AA-6 exhibited higher tolerance to ILs and enhanced thermo stability in the presence of high saline conditions.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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