Antibiotic resistant bacterial population is a great threat for human as well as for other domestic animals. Unscientific
use of antibiotics in clinical sectors create a selective pressure on bacteria that make bacteria resistant to a wide range
of drugs. The current finding demonstrated the isolation and characterization of multi antibiotics resistant bacterial
species from oil contaminated soil and its lateral gene transfer efficiency at different temperature. Among six bacterial
isolates, the strain OD1 showed highest antibiotic resistant capacity was selected for further studies. Morphological,
biochemical and 16S rDNA sequence analysis confirmed the bacterial strains as Alcaligenes faecalis strain OD1. It was
recorded that antibiotics resistant gene transfer was highly dependent on temperature and showed maximum transfer
efficiency at 25°C (9.6 ×10-6).