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Effects of acids pre-treatment on the microbial fermentation process for bioethanol production from microalgae

Phwan CK 1 , Chew KW 2 , Sebayang AH 3 , Ong HC 3 , Ling TC 1 , Malek MA 4 Show all authors , Ho YC 5 , Show PL 2

Affiliations 

  • 1 1Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • 2 2Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan Malaysia
  • 3 3Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • 4 4Institute of Sustainable Energy (ISE), Universiti Tenaga Nasional, 43000 Kajang, Selangor Malaysia
  • 5 5Civil and Environmental Engineering Department, Universiti Teknologi PETRONAS, 32610 Seri Iskandar, Perak Darul Ridzuan Malaysia
Biotechnol Biofuels, 2019;12:191.
PMID: 31384298 DOI: 10.1186/s13068-019-1533-5

Abstract

Background: Microalgae are one of the promising feedstock that consists of high carbohydrate content which can be converted into bioethanol. Pre-treatment is one of the critical steps required to release fermentable sugars to be used in the microbial fermentation process. In this study, the reducing sugar concentration of Chlorella species was investigated by pre-treating the biomass with dilute sulfuric acid and acetic acid at different concentrations 1%, 3%, 5%, 7%, and 9% (v/v).

Results: 3,5-Dinitrosalicylic acid (DNS) method, FTIR, and GC-FID were employed to evaluate the reducing sugar concentration, functional groups of alcohol bonds and concentration of bioethanol, respectively. The two-way ANOVA results (p 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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