Affiliations 

  • 1 School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University (SJTU), 1954 Huashan Road, Shanghai 200030, China
  • 2 Department of Biology, University of Hafr Al Batin, Hafar Al-batin 39524, Saudi Arabia
  • 3 Department of Industrial Biotechnology, Atta ur Rahman School of Applied Biosciences, National University of Sciences and Technology, H-12, Islamabad 44000, Pakistan
  • 4 School of Biological Sciences, University of the Punjab, Lahore 54590, Pakistan
  • 5 Centre for Advanced Composite Materials, Universiti Teknologi Malaysia Skudai, Skudai 81310, Malaysia
  • 6 School of Biomedical Engineering and Health Sciences, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai 81300, Malaysia
Materials (Basel), 2020 Feb 21;13(4).
PMID: 32098139 DOI: 10.3390/ma13040971

Abstract

Advancement and development in bone tissue engineering, particularly that of composite scaffolds, are of great importance for bone tissue engineering. We have synthesized polymeric matrix using biopolymer (β-glucan), acrylic acid, and nano-hydroxyapatite through free radical polymerization method. Bioactive nanocomposite scaffolds (BNSs) were fabricated using the freeze-drying method and Ag was coated by the dip-coating method. The scaffolds have been characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction analysis (XRD) to investigate their functional groups, surface morphology, and phase analysis, respectively. The pore size and porosity of all BNS samples were found to be dependent on silver concentration. Mechanical testing of all BNS samples have substantial compressive strength in dry form that is closer to cancellous bone. The samples of BNS showed substantial antibacterial effect against DH5 alpha E. coli. The biological studies conducted using the MC3T3-E1 cell line via neutral red dye assay on the scaffolds have found to be biocompatible and non-cytotoxic. These bioactive scaffolds can bring numerous applications for bone tissue repairs and regenerations.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.