Affiliations 

  • 1 Department of Polymer Engineering and Technology, University of the Punjab, Lahore, Pakistan
  • 2 Department of Metallurgy and Materials Engineering, CEET, University of the Punjab, Lahore, Pakistan
  • 3 School of Biomedical Engineering and Health Sciences, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia
  • 4 Department of Biological Sciences, National University of Medical Sciences, Rawalpindi, Punjab, Pakistan
  • 5 Nanotechnology and Biomaterials Lab, Physics Department, Forman Christian College University, Lahore, Pakistan
  • 6 School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia
  • 7 Department of Chemical Engineering, College of Engineering, King Saud University, Riyadh, Saudi Arabia
  • 8 Sustainable Energy Technologies (SET) Center, College of Engineering, King Saud University, Riyadh, Saudi Arabia
  • 9 School of Biological Sciences, University of the Punjab, Lahore, Pakistan
J Tissue Eng Regen Med, 2020 10;14(10):1488-1501.
PMID: 32761978 DOI: 10.1002/term.3115

Abstract

It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease. This work is focused on the preparation of a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers. Fourier transform infrared spectroscopy (FT-IR) confirmed that both ARX and GG interacted well with each other, and their interactions further increased with the addition of crosslinker tetraethyl orthosilicate. Scanning electron microscope (SEM) micrographs showed uniform porous morphologies of the hydrogels. The porous morphologies and uniform interconnected pores are attributed to the increased crosslinking of the hydrogel. Elastic modulus, tensile strength, and fracture strain of the hydrogels significantly improved (from ATG-1 to ATG-4) with crosslinking. Degradability tests showed that hydrogels lost maximum weight in 7 days. All the samples showed variation in swelling with pH. Maximum swelling was observed at pH 7. The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior. The cells not only retained their cylindrical morphologies onto the hydrogel but were also performing their normal activities. It is, therefore, believed that as-developed hydrogel could be a potential material for wound dressing application.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.