The present work was carried out to formulate a pasta with oyster mushroom flour (OMF), and to evaluate the effect of its addition on the nutritional compositions, physicochemical properties, and sensorial acceptability of the formulated pasta. Four pasta formulations with different levels of OMF (0%, 5%, 10%, and 15%) were developed prior to the determination of the nutritional composition, physicochemical properties, and sensorial acceptability. Proximate components such as protein, ash, and fat increased with increasing OMF levels except for carbohydrates and energy, which were decreased with increasing OMF levels. Lightness (L*) and yellowness (b*) significantly decreased with increasing OMF levels, while redness (a*) increased with increasing OMF levels. The results showed that the optimum cooking time, elasticity, firmness, and shear work decreased with increasing OMF levels, while there were no significant differences in hardness or adhesiveness between the samples. In sensory evaluation, the pasta fortified with up to 10% OMF was generally well accepted by the sensory panellists. The addition of 10% OMF into the pasta resulted in an increase in its nutritional values, thus affecting its physicochemical properties, and improving some of its sensorial attributes.