Prolonged standing has been identified as one of the risk factors which is associated with occupational injuries. Therefore, the primary aim for this study is to determine the association between prolonged standing and muscle dis- comfort among workers who have to stand for long periods of time. A total of 74 respondents were given and asked to complete a self-administered Borg’s Scale questionnaire. After that, the completed questionnaire was analysed using statistical analysis. This study highlighted the correlation between height, weight and age of the respondents with muscle discomfort following exposure to prolonged standing. Besides that, the association between prolonged standing and muscle discomfort also will be highlighted. In short, the prevalence of leg muscle discomfort was higher compared to the muscle discomfort experienced by the other body parts.