Prolonged standing has been identified as one of the risk factors which is associated with occupational injuries. Therefore, the primary aim for this study is to determine the association between prolonged standing and muscle dis- comfort among workers who have to stand for long periods of time. A total of 74 respondents were given and asked to complete a self-administered Borg’s Scale questionnaire. After that, the completed questionnaire was analysed using statistical analysis. This study highlighted the correlation between height, weight and age of the respondents with muscle discomfort following exposure to prolonged standing. Besides that, the association between prolonged standing and muscle discomfort also will be highlighted. In short, the prevalence of leg muscle discomfort was higher compared to the muscle discomfort experienced by the other body parts.
Ergonomics is a science focusing on the study of human fit, decreasing human fatigue and discomfort through the design of new product. Prevention related to workers injury and illness such as muscle discomfort is part of the main goals in ergonomics interventions. Thus, this present study investigates the effectiveness of ergonomic interventions such as footrest and floor conditions in reducing workers lower leg muscle discomforts during prolonged standing. The main objective of this study was to determine and compare the lower leg muscles discomfort (exertion percent- age (%)) of Gastrocnemius and Tibialis Anterior among the respondents using the two ergonomic interventions (foot- rest and anti-fatigue mat). About 60 healthy subjects were recruited to stand for 2 hours (120 minutes) while using the two interventions in different session with one week interval between each test session. During standing, lower leg muscle discomfort of Gastrocnemius and Tibialis Anterior muscles were continuously monitored. Changes in lower leg muscle discomforts over standing time were measured using the surface Electromyography (sEMG). In this study, the sEMG readings showed that the percentage of exertion (%) were increasing with time (within 120 minutes) on muscles for both legs with the usage of the interventions (footrest and anti-fatigue mat). However, the percentage of exertion (%) from the sEMG readings were lower compared to previous studies. The independent t-test was used to find the mean changes on exertion percentage (%) between each muscles of both legs for the two interventions. Results found that there were significant exertion percentage at certain time with 15 minutes time period within the 120 minutes standing. This study showed that the ergonomic interventions (anti-fatigue mat and footrest) gives a low number of exertion percentage (%), showing a reduced muscle discomfort to the lower leg muscles compared to previous studies and interventions. In comparisons with footrest, this study showed that anti-fatigue mat is more applicable for the assembly workers in the industrial factory. The data produced by the comparisons between the two interventions can be useful especially to the Department of Occupational Safety and Health Malaysia (DOSH) in enhancing the safety and wellbeing of industrial workers in Malaysia.
Calcium carbonate (CaCO3
) plays a crucial role in influencing the growth of osteoblast. This study was conducted
to compare the performance of alginate/cockle shell powder nanobiocomposite (nCP) bone scaffold developed from
naturally occurring CaCO3 with alginate/calcium carbonate (CC) bone scaffold developed using synthetic CaCO3. The
study compares the performance of the scaffold in supporting the growth of osteoblast through in vitro evaluations as
well as initial biocompatibility observations through in vivo methods. Both scaffolds were developed using the mixture
of 40% alginate solution with either 60% of nano cockle shell powder or synthetic CaCO3 to obtain a three dimensional
scaffold structure. In vitro evaluation on calcium release and ALP enzyme activity was conducted on both scaffolds seeded
with osteoblast on day’s three, five and seven using commercial kits. In vivo observations using histological methods
were further conducted by implanting osteoblast seeded scaffold subcutaneously at the dorsum of 8 albino mice for 21
days. Findings from in vitro studies showed a significant increase (p < 0.05) in the release of calcium and ALP enzyme
activity in nCP scaffolds on day seven compared to days three and five of CC scaffold. Histological observations using
H&E and von Kossa staining showed infiltration and proliferation of osteoblast on both scaffolds as well as early stage
bone tissue formation. Formation of new blood vessels within the scaffolds was also observed in nCP scaffold. Both the
developed scaffolds were noted to support osteoblast growth and new tissue formation with better potentials displayed by
nCP scaffolds comparatively. This study shows that naturally occurring CaCO3 obtained from cockle shells in the form
of nano powder has good potentials to be used as a biomaterial for bone tissue engineering applications.
Nur Khalisah Kaswan, Nurul Syazwani Mohd Suhaimi, Noor Aishah Mohammed Izham, Tengku Azam Shah Tengku Mohamad, Mohd Roslan Sulaiman, Enoch Kumar Perimal
Background: Cardamonin is a naturally occurring chalcone from the Alpinia species. It is known to possess antioxidant and anti-inflammatory properties. Our previous studies have shown that cardamonin has antihyperalgesic and antiallodynic effects on CCI-induced neuropathic pain in mice. Although the evidence of the association between cardamonin and neuropathic pain has been reported in animal studies, specific targets using in vitro models are still lacking. Objectives/Methods: This study aims to investigate the effect of cardamonin on nitric oxide production using the LPS-induced neuropathic pain-like SH-SY5Y in vitro model through NMDA receptor expression. Results: Cardamonin administration in differentiated SH-SY5Y cells significantly reduced nitric oxide production assessed using Griess reagent. Western blot analysis demonstrated a significant reduction in GluN2B receptor expression in the cardamonin treated SHSY5Y cells compared to the vehicle treated group. Conclusions: These data suggest that cardamonin reduces nitric oxide production modulated through NMDA GluN2B receptor subunit. Our results provides preliminary data to support the in vivo studies using cardamonin and may contribute to further understanding the mechanisms of action of cardamonin.
Boesenbergia rotunda, traditionally used to relieve stomach, abdomen, joint, muscle, and rheumatic pain was also reported for its antinociceptive effect on a mouse model. However, the possible pain relief effect of Boesenbergia rotunda ethanolic extract (BREE) via the inhibition to the neural pain pathway remains to be elucidated. This study investigated the inhibitory effect of BREE on compound action potentials (CAPs) and the possible involvement of the opioid receptors. The changes in the CAPs amplitudes of the frog’s sciatic nerves were evaluated following the exposure to three different dosages of BREE (1, 3 and 10 mg/ml and morphine (3 mg/ml). In another set of experiment, the nerves were pretreated with a non-selective opioid receptor antagonist, naloxone (0.1 mg/ml), before exposing the nerve to BREE (1 mg/ml) to investigate the involvement of opioid receptors in the CAPs inhibitory mechanism. The outcome showed a reduction in the CAPs amplitudes when treated with BREE (1, 3 and 10 mg/ml) whereby the effect was reversible. The CAPs inhibition by BREE was absent when the opioid receptors were blocked. Taken together, these findings suggest that BREE-induced CAPs amplitude reduction involves the activation of opioid receptors.