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A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2

Lau YL 1 , Ismail IB 2 , Izati Binti Mustapa N 2 , Lai MY 1 , Tuan Soh TS 2 , Hassan AH 2 Show all authors , Peariasamy KM 3 , Lee YL 3 , Goh PP 4

Affiliations 

  • 1 Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
  • 2 Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia
  • 3 Clinical Research Centre, Hospital Sungai Buloh, Selangor, Malaysia
  • 4 Institute for Clinical Research (ICR), National Institutes of Health (NIH), Ministry of Health Malaysia, Putrajaya, Malaysia
Am J Trop Med Hyg, 2020 Dec;103(6):2350-2352.
PMID: 33098286 DOI: 10.4269/ajtmh.20-1079

Abstract

A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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