Fulltext

Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Lai MY 1 , Bukhari FDM 1 , Zulkefli NZ 1 , Ismail I 2 , Mustapa NI 2 , Soh TST 2 Show all authors , Hassan AH 2 , Peariasamy KM 3 , Lee YL 4 , Suppiah J 5 , Thayan R 5 , Lau YL 6

Affiliations 

  • 1 Department of Parasitology, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia
  • 2 Department of Pathology, Hospital Sungai Buloh, Ministry of Health, Malaysia
  • 3 Institute for Clinical Research, National Institutes of Health, Ministry of Health, Malaysia
  • 4 Clinical Research Centre, Hospital Sungai Buloh, Ministry of Health, Malaysia
  • 5 Virology Unit, Infectious Disease Research Centre, Institute for Medical Research, National Institutes of Health, Ministry of Health, Malaysia
  • 6 Department of Parasitology, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: lauyeeling@um.edu.my
Int J Infect Dis, 2022 Jul;120:132-134.
PMID: 35472524 DOI: 10.1016/j.ijid.2022.04.036

Abstract

OBJECTIVES: Preventing reverse transcription loop-mediated isothermal amplification (RT-LAMP) carryover contamination could be solved by adding deoxyuridine triphosphate (dUTP) and uracil-DNA glycosylase (UDG) into the reaction master mix.

METHODS: RNA was extracted from nasopharyngeal swab samples by a simple RNA extraction method.

RESULTS: Testing of 77 samples demonstrated 91.2% sensitivity (95% confidence interval [CI]: 78-98.2%) and 100% specificity (95% confidence interval: 92-100%) using UDG RT-LAMP.

CONCLUSION: This colorimetric UDG RT-LAMP is a simple-to-use, fast, and easy-to-interpret method, which could serve as an alternative for diagnosis of SARS-CoV-2 infection, especially in remote hospitals and laboratories with under-equipped medical facilities.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

MeSH terms
Similar publications