Affiliations 

  • 1 Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Sarawak, Malaysia. Electronic address: 19010029@siswa.unimas.my
  • 2 Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Sarawak, Malaysia
  • 3 Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Sarawak, Malaysia. Electronic address: hhchung@unimas.my
  • 4 School of Public Health, The University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong
  • 5 Department of Science and Environmental Studies, The Education University of Hong Kong, Tai Po Campus, NT, Hong Kong
Comput Biol Chem, 2020 Dec;89:107403.
PMID: 33120127 DOI: 10.1016/j.compbiolchem.2020.107403

Abstract

The Blueline Rasbora (Rasbora sarawakensis) is a small ray-finned fish categorized under the genus Rasbora in the Cyprinidae family. In this study, the complete mitogenome sequence of R. sarawakensis was sequenced using four primers targeting overlapping regions. The mitogenome is 16,709 bp in size, accommodating 22 transfer RNA genes, 13 protein-coding genes, two ribosomal RNA genes and a putative control region. Identical gene organisation was detected between this species and other genus counterparts. The heavy strand houses 28 genes while the light strand stores the other nine genes. Most protein-coding genes employ ATG as start codon, excluding COI gene, which utilizes GTG instead. The central conserved sequence blocks (CSB-F, CSB-E and CSB-D), variable sequence blocks (CSB-3, CSB-2 and CSB-1) as well as the terminal associated sequence (TAS) are conserved in the control region. The maximum likelihood phylogenetic tree revealed the divergence of R. sarawakensis from the basal region of the Rasbora clade, where its evolutionary relationships with R. maculatus and R. pauciperforata are poorly resolved as indicated by the low bootstrap values. This work acts as steppingstone towards further molecular evolution and population genetics studies of Rasbora genus in future.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.