Affiliations 

  • 1 Faculty of Food Science and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus 21030, Malaysia
  • 2 Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture, Makurdi P.M.B. 2373, Nigeria
Vet Sci, 2021 May 04;8(5).
PMID: 34064306 DOI: 10.3390/vetsci8050075

Abstract

This study was designed to examine the use of RAPD markers in discriminating triploid and diploid African catfish Clarias gariepinus (Burchell, 1822). Following a routine technique, triploidy was induced by cold shock and confirm by erythrocyte measurement in C. gariepinus. Thereafter, 80 RAPD markers were screened; out of which, three showed the highest percentage of polymorphism (i.e., OPB 16 = 71.43%; OPC 14 = 61.9%; OPD 12 = 75%). The results obtained showed genotype differences between triploid and diploid without overlapping. However, the development of a Sequence Characterized Amplified Region (SCAR) marker was not achievable because progenies of triploid and diploid C. gariepinus could not be differentiated based on a specific fragment. Consequently, the genetic distance showed high similarities for both treatments and the UPGMA-generated dendrogram could not separate the treatments into two distinct clusters. It was concluded that RAPD makers cannot be used to separate the ploidy status of fishes.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.