Affiliations 

  • 1 Tissue Engineering Centre, Faculty of Medicine, Universiti Kebangsaan Malaysia, Clinical Block, Jalan Yaacob Latiff, Cheras, Kuala Lumpur 56000, Malaysia
  • 2 Department of Orthopedic & Traumatology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Clinical Block, Jalan Yaacob Latiff, Cheras, Kuala Lumpur 56000, Malaysia
  • 3 Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
  • 4 Biomaterial Group, R&D Center, Nitta Gelatin Inc., 2-22, Futamata, Yao City, Osaka 581-0024, Japan
Biomedicines, 2021 Jul 23;9(8).
PMID: 34440084 DOI: 10.3390/biomedicines9080880

Abstract

The tissue engineering approach in osteoarthritic cell therapy often requires the delivery of a substantially high cell number due to the low engraftment efficiency as a result of low affinity binding of implanted cells to the targeted tissue. A modification towards the cell membrane that provides specific epitope for antibody binding to a target tissue may be a plausible solution to increase engraftment. In this study, we intercalated palmitated protein G (PPG) with mesenchymal stem cells (MSCs) and antibody, and evaluated their effects on the properties of MSCs either in monolayer state or in a 3D culture state (gelatin microsphere, GM). Bone marrow MSCs were intercalated with PPG (PPG-MSCs), followed by coating with type II collagen antibody (PPG-MSC-Ab). The effect of PPG and antibody conjugation on the MSC proliferation and multilineage differentiation capabilities both in monolayer and GM cultures was evaluated. PPG did not affect MSC proliferation and differentiation either in monolayer or 3D culture. The PPG-MSCs were successfully conjugated with the type II collagen antibody. Both PPG-MSCs with and without antibody conjugation did not alter MSC proliferation, stemness, and the collagen, aggrecan, and sGAG expression profiles. Assessment of the osteochondral defect explant revealed that the PPG-MSC-Ab micromass was able to attach within 48 h onto the osteochondral surface. Antibody-conjugated MSCs in GM culture is a potential method for targeted delivery of MSCs in future therapy of cartilage defects and osteoarthritis.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.