An extractive fermentation technique was developed using a thermoseparating reagent to form a two-phase system for simultaneous cell cultivation and downstream processing of extracellular Burkholderia cepacia lipase. A 10% (w/w) solution of ethylene oxide-propylene oxide (EOPO) with a molecular mass of 3900 g/mol and pH 8.5, a 200 rpm speed, and 30 °C were selected as the optimal conditions for lipase production (55 U/ml). Repetitive batch fermentation was performed by continuous replacement of the top phase every 24h, which resulted in an average cell growth mass of 4.7 g/L for 10 extractive batches over 240 h. In scaling-up the process, a bench-scale bioreactor was tested under the conditions that had been optimized in flasks. The production rate and recovery yield were higher in the bioreactor compared to fermentation performed in flasks.
The present study aimed to assess the effects of anthropogenic activities on the heavy metal levels in the Langat River by transplantation of Corbicula javanica. In addition, potential ecological risk indexes (PERI) of heavy metals in the surface sediments of the river were also investigated. The correlation analysis revealed that eight metals (As, Co, Cr, Fe, Mn, Ni, Pb and Zn) in total soft tissue (TST) while five metals (As, Cd, Cr, Fe and Mn) in shell have positively and significantly correlation with respective metal concentration in sediment, indicating the clams is a good biomonitor of the metal levels. Based on clustering patterns, the discharge of dam impoundment, agricultural activities and urban domestic waste were identified as three major contributors of the metals in Pangsun, Semenyih and Dusun Tua, and Kajang, respectively. Various geochemical indexes for a single metal pollutant (geoaccumulation index (I geo), enrichment factors (EF), contamination factor (C f) and ecological risk (Er)) all agreed that Cd, Co, Cr, Cu, Fe, Mn, Ni and Zn are not likely to cause adverse effect to the river ecosystem, but As and Pb could pose a potential ecological risk to the river ecosystem. All indexes (degree of contamination (C d), combined pollution index (CPI) and PERI) showed that overall metal concentrations in the tropical river are still within safe limit. River metal pollution was investigated. Anthropogenic activities were contributors of the metal pollution. Geochemical indexes showed that metals are within the safe limit.
A bulk YBa2Cu3O7-δ (Y-123) superconductor synthesized by a thermal treatment method was added with different weight percentages (x = 0.0, 0.2, 1.0, 1.5, and 2.0 wt.%) of BiFeO3 (BFO) nanoparticle. X-ray diffraction (XRD), alternating current susceptibility (ACS), and field emission scanning electron microscopy (FESEM) were used to determine the properties of the samples. From the XRD results, all samples showed an orthorhombic crystal structure with a Pmmm space group. The sample x = 1.0 wt.% gave the highest value of Y-123. The high amounts of BFO degraded the crystallite size of the sample, showing that the addition did not promote the grain growth of Y-123. From ACS results, the Tc-onset value was shown to be enhanced by the addition of the BFO nanoparticle, where x = 1.5 wt.% gave the highest Tc value (91.91 K). The sample with 1.5 wt.% showed a high value of Tp (89.15 K). The FESEM analysis showed that the average grain size of the samples decreased as BFO was introduced. However, the small grain size was expected to fill in the boundary, which would help in enhancing the grain connectivity. Overall, the addition of the BFO nanoparticles in Y-123 helped to improve the superconducting properties, mainly for x = 1.5 wt.%.