Crab meat is widely consumed in several countries around the world. However, when consumed, crab meat are frequent cause of allergic reactions throughout the world. Scylla serrata is among the most common mud crab in Malaysia. In a previous study two major allergens of mud crab at 36 and 41 kDa was identified. Thus, the aim of this study is to further identify these major allergens by a proteomic approach. Protein extract was prepared and resolved by 2-dimensional electrophoresis (2-DE). Immunoblotting was then performed using reactive sera from patients with crab allergy. Major allergenic spots were then excised from the 2-DE gel and analysed by mass spectrometry. The 2-DE profile of the extract revealed approximately >100 protein spots between pH of 4.00 to 8.00. Mass spectrometry analysis has identified the 36 and 41 kDa proteins as tropomyosin and arginine kinase, respectively. Our findings indicated that tropomyosin and arginine kinase play a major role in allergic reaction to mud crab meat among local patients with crab meat allergy, and should be included in diagnostics and therapeutic strategies of this allergy.
The state space modeling approach was developed to cope with the demand
and performance due to the increase in system complexity, which may have
multiple inputs and multiple outputs (MIMO). This approach is based on timedomain
analysis and synthesis using state variables. This paper describes the
development of a state space representation of a furnace system of a combined
cycle power plant. Power plants will need to operate optimally so as to stay
competitive, as even a small improvement in energy efficiency would involve
substantial cost savings. Both the quantitative and qualitative analyses of the
state space representation of the furnace system are discussed. These include
the responses of systems excited by certain inputs and the structural properties
of the system. The analysis on the furnace system showed that the system is
bounded input and bounded output stable, controllable and observable. In
practice, the state space formulation is very important for numerical computation
and controller design, and can be extended for time-varying systems.
This paper provides a review on the effect of solar radiation to the different building materials properties.
Solar radiation; watt per meter square [W/m2
] is one of the cause for thermal gain in building envelopes.
Buildings envelopes comprises of various materials. Different materials have different rate of heat
absorption depends on their emissivity and other parameters. The three materials studied in this paper
are concrete, timber and composites materials. According to the radiation heat equation, heat rate are
affected by the surface area of exposed envelope (A) measure in meter (m), emissivity of the building
exposed surface (ε) and the temperature difference between envelope exposed surface (Ts) and
temperature of equivalent atmosphere (Tsky) measure in 0C. Based on the parameters, research
methodology were adopted either by software simulation or test field experimental. Solar radiation
affects the materials in various ways, depends on parameters considered, location of testing and type of
materials.
The new complexes [CuL2
(H2
O)2
] and [FeL2
(CH3
O)2
] in which L =
β-mangostin were synthesised and characterised. The structure of the
ligand, β-mangostin was confirmed using NMR and the purity of ligand
was determined using HPLC. Both Cu(II) and Fe(II) complexes were
prepared by reaction between the ligand and the acetate of the metals in
one-step reaction. The synthesised compounds have been characterised
using UV-Visible, FTIR and CHNS analyser. Ligand and metal complexes
were tested against bacteria to assess on their antimicrobial properties using
Minimum Inhibitory Concentrations (MICs) and Minimum Bactericidal
Concentrations (MBCs) method. The elemental analysis and spectra data
suggested octahedral geometry for both Cu(II) and Fe(II) complexes. The
IR spectroscopy revealed that the chelation of Cu2+ and Fe2+ ion occurred
with hydroxyl and carbonyl group at C9
and C1 respectively of β-mangostin.
Both Cu(II) and Fe(II) complexes showed stronger inhibition against
Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae and
Salmonella pneumonia at concentration 900 mg/mL and Escherichia coli
at 450 mg/mL compared to the ligand itself
Anion clay hydrotalcite MgA1C0 3 with a Mg/A1 molar ratio of 3:1 was synthesized by co-precipitation at room temperature and pressure. The physicochemical properties were evaluated using Powder X-Ray Diffraction (PXRD), Fourier Transform Infrared (FTIR) spectroscopy and Thermogravimetric Analysis (TGA). The efficacy of anion clay hydrotalcite in the removal ofCcf- from aqueous solutions was investigated with respect to contact time, initial concentration, pH, adsorbent dosage and temperature. The Cd2- removal increased with the increased in contact time, adsorbent dosage, pH and initial concentration. Adsorption decreases with increasing initial concentration and temperature, for which the latter is indicative of an exothermic process. The equilibrium adsorption capacity of MgA1C0 3 was evaluated using linear Langmuir and Freundlich isotherms with respect to the separation factor, RL.
Sewage sludge is a by-product generated within the wastewater treatment process. Severe concern arised as the sludge are massively been dumped to the landfill and it may affect the environment. Many studies had been conducted in reusing the sewage sludge as construction material, where it is one of the optional ways to solve the issue. In this study, dried sewage sludge was incinerated with two different temperatures in order to produce sewage sludge ash (SSA), which are 800°C and 1000°C. Afterfew processes, this SSA then reused in mortar as cementreplacement with the replacement percentage of 5%, 10%, 15% and 20% by weight. The strength performance of mortar specimens was investigated after 7, 28, 60 and 90 days of curing. From the results, it is clearly showed that the compressive strength of all mortar specimens increased when the period of curing was prolonged. Moreover, almost all compressive strength of SSA mortars was higher than the control mortar. Therefore, there is potential to reuse this waste material as part of construction materials and hence, its plays an important role for future researches in minimisation of waste.
Palm Oil Mill Effluent (POME) is the largest contributor of biomass from
the palm oil milling industry. Conventional method of POME treatment
using ponding system should be improved because of huge land resource
requirement. In this study, microbubbles technology was applied to
understand the recovery rate of residual oil from POME at different
operating temperatures. Temperature for POME was set at 27 oC, 30 oC and
50 oC to determine the microbubble size distributions and characteristics at
different POME temperature. At each temperature, the size of microbubbles
was measured based on six size range; 50 μm. The results showed that at different
temperatures, the microbubbles size distribution varies and the smallest
group of microbubbles (
The cloud point extraction (CPE) method was developed to determine the zinc prior to Ultraviolet-visible (UV-Vis) spectrophotometry detection. Triton X-100was applied as extractant based on the complexation reaction of Zn(II) ions with ethylenediaminetetraacetic acid (EDTA). Under optimal conditions, the CPE was used to determine the concentration of zinc in canned food samples. The amounts of zinc found in the food samples were in the range of 0.005-0.007 mg/L with RSD of < 8 %. This confirmed that the proposed CPE method is suitable for the determination of zinc in food samples, indicating the concentration of zinc was within the permissible limit.
This paper presents the application of Solid Phase Micro Extraction (SPME) coupled with Gas Chromatography - Mass Spectrometry (GC-MS) and Gas Chromatography - Flame Ionization Detector (GC-FID) in characterizing the agarwood incense. The work involved three types of SPME fibres at 30 minute sampling time. The fibres are 50/30 μm divinylbenzene-carboxen-polydimethysiloxane (DVB-CAR-PDMS), 65 μm polydi methylsiloxane-divinylbenzene (PDMS-DVB) and 85 μm carboxen-polydimethyl siloxane (CAR-PDMS). The results showed that among the many compounds extracted by GC-MS coupled with SPME, six compounds were substantially found in high quality agarwood incense due to their high percentage area (%). They are β-maaliene, α-elemol, β-selinene, 10-epi-γ-eudesmol, agarospirol and caryophellene oxide. The finding offers a new approach for establishing the volatile profile of agarwood incense components as well as for agarwood grading and discrimination.
Text localisation determines the location of the text in an image. This process
is performed prior to text recognition. Localising text on shop signage is
a challenging task since the images of the shop signage consist of complex
background, and the text occurs in various font types, sizes, and colours.
Two popular texture features that have been applied to localise text in
scene images are a histogram of oriented gradient (HOG) and speeded up
robust features (SURF). A comparative study is conducted in this paper
to determine which is better with support vector machine (SVM) classifier.
The performance of SVM is influenced by its kernel function and another
comparative study is conducted to identify the best kernel function. The
experiments have been conducted using primary data collected by the
authors. Results indicate that HOG with quadratic kernel function localises
text for shop signage better than SURF.
Vitex trifolia or locally known as lemuni, is well documented for its medicinal properties including
anticancer, antibacterial and wound healing. For years, the leaves and flowers were consumed for antiaging
and general well-being. It is also commonly served as nutraceuticals to post-partum women during
their confinement period. Previous study showed that V. trifolia possesses high antioxidant properties that
could prevent oxidative stress related diseases such as cancer, atherosclerosis, diabetes and many more.
From the literature, V. trifolia leaves are used as poultice for rheumatic pains, sprains, antithelmintics and
inflammations. Meanwhile, the fruits are utilised in amenorrhea. The roots, on the other hand, are used for
the treatment of cough and fever. The plant is known to possess pharmacologically-active constituents such
as the essential oil, halimane-type diterpenes, flavonoids, triterpenes, lignans, iridoids and ecdysteroids.
Review papers were consistently published to justify the non-exhaustive biological exploration of this genus.
Nevertheless, only 11 per cent of the total Vitex species were phytochemically examined. Apart from being
eaten raw or blanched, Vitex is also taken as a food colourant in preparing lemuni rice. The process of
making lemuni rice is quite tedious. First, the leaves and flowers are picked and washed. Both parts are then
ground with water and sieved to give a bluish extract which is then mixed with rice and boiled to cook.
Hence, the Vitex pouch is introduced to provide an easy way to prepare lemuni rice. This packaging is
designed to enclose Vitex samples in a pouch that simply can be placed together with rice and boiled to
cook. Such instant preparation will hopefully promote the consumption of lemuni rice among busy locals.
Future plans include international marketing and production of other Vitex nutritional products.
'Doa' is derived from Arabic word which means that one asks for the
fulfillment or a need or the cure of sickness from him/her. Having to search
and retrieve the relevant ‘doa’ for one needs at any particular time is
beneficial. There are some search and retrieval applications that require
using the exact match of the keyword search with the words stored in the
database. This approach leads to the retrieval of insignificant results as
users need to know the exact word to be searched. Therefore, this project
allows for partial keyword search that utilises N-gram method for the
search and retrieval process. Moreover, various words may have similar
meaning thus to increase the accuracy of the retrieved result, this project
compares the dice and overlap coefficient algorithms to find the synonyms
of the searched word. The result produced indicates that overlap coefficient
perform better than dice coefficient.
Composite panels were manufactured from kenaf particles and treated with two different alkali treatments using 2% NaOH and 2% KOH with resin contents of 8% and 10% of phenol formaldehyde (PF) at medium density of 650kg/m3. The objectives of this study were to determine the mechanical properties in terms of its modulus of rupture (MOR), modulus of elasticity (MOE) and internal bond (IB), and physical properties namely thickness swelling (TS) and water absorption (WA) of treated kenaf board. The mechanical and physical tests were performed according to the Malaysian Standard (MS1787:2004). The minimum requirements value for MOE, MOR and IB were 2000 MPa, 14 MPa and 0.45 MPa respectively for furniture grade particleboards for use in humid conditions (PF2). According to Malaysian specifications for physical properties, the maximum requirement for thickness swell is 15%. Results indicated that both treated boards with NaOH and KOH showed an increase in strength properties compared to untreated particleboard. Particleboard treated with KOH exhibited the highest MOR and MOE values, while board with NaOH treatment gave the highest IB value. The boards with treated particles gave better performance in terms of physical properties. There were no significant differences in mechanical properties (MOR, MOE and IB) and physical properties for the different alkali treatment. The values of bending strength and IB strength increased with an increase in resin content, while TS and WA increased with a decrease in resin content. In conclusion, NaOH and KOH treated kenaf particles improved board performance and could be considered as an alternative material for particleboard production.
This study focused on the identification of pathogenic bacteria in raw water intake and after sand filtration for drinking water treatment plant during flood event in 2014. The samples was collected from the Lubok Buntar Water Treatment Plant (WTP) and processed through bacterial isolation using chocolate agar as a media. The isolation process conducted based on serial samples dilution and streaking method prior to DNA extraction. Deoxyribonucleic acid (DNA) extraction kit was used to get selected bacteria DNA and further analysis using Polymerase Chain Reaction (PCR) test and electrophoresis to get DNA sequences. The Basic Local Alignment Search Tool (BLAST) analysis was employed to identify the species of the isolated bacteria. As a result, Pantoeaagglomerans and Enterobacter sp. were found in raw and filtered water sample and indicating the same family types. It was concluded that bacteria of the same species were found before and after sand filtration and need to be removed by disinfectant process. The findings also indicated that all the physicochemical parameters measured were within the values prescribed by the Interim National Water Quality Standard (INWQS).
Channel estimation techniques for Multiple-input Multiple-output Orthogonal Frequency Division Multiplexing (MIMO-OFDM) based on comb type pilot arrangement with least-square error (LSE) estimator was investigated with space-time-frequency (STF) diversity implementation. The frequency offset in OFDM impacts its performance. This was mitigated with the implementation of the presented inter-carrier interference self-cancellation (ICI-SC) techniques and different space-time subcarrier mapping. STF block coding in the system exploits the spatial, temporal and frequency diversity to improve performance. Estimated channel was fed into a decoder which combines the STF decoding together with the estimated channel coefficients using LSE estimator for equalization. The performance of the system is compared by measuring the symbol error rate with a PSK-16 and PSK-32. The results show that subcarrier mapping together with ICI-SC were able to increase the system performance. Introduction of channel estimation was also able to estimate the channel coefficient at only 5dB difference with a perfectly known channel.
A ribbed slab structure has the advantage in the reduction of concrete volume in between the ribs resulting in a lower structural self-weight. In order to overcome the drawbacks in the construction process, the application of steel fibre self-compacting concrete (SCFRC) is seen as an alternative material to be used in the slab. This preliminary investigation was carried out to investigate the flexural behaviour of steel fibre selfcompacting concrete (SCFRC) asthe main material in ribbed slab omitting the conventionalreinforcements. Two samples ofribbed slab were prepared for this preliminary study; 2-ribbed and 3-ribbed in 1 m width to identify the effect of the geometry to the slab’s flexural behaviour. The dimension of both samples is 2.5 m x 1 m with 150 mm thickness. The compressive strength of the mix is 48.6 MPa based on the cubes tested at 28 days. Load was applied to failure by using the four point bending test set-up with simple support condition. The result of the experiment recorded ultimate load carrying capacity at 30.68 kN for the 2-ribbed slab and 25.52 kN for 3-ribbed slab. From the results, the ultimate load of the 2-ribbed sample exceeds 3-ribbed by approximately 20%. This proved that even with lower concrete volume, the sample can still withstand an almost similar ultimate load. Cracks was also observed and recorded with the maximum crack width of 2 mm. It can be concluded that the steel fibres do have the potential to withstand flexural loadings. Steel fibre reduces macro-crack forming into micro-cracks and improves concrete ductility, as well as improvement in deflection. This shows that steel fibre reinforced self-compacting concrete is practical as it offers good concrete properties as well as it can be mixed, placed easier without compaction.
Storytelling is considered as an interactive social arts that uses word and
gestures to reveal the elements and images of a story while engaging the
listener's imagination. Multimedia based digital storytelling learning
approach provides interesting, interactive, engaging and multisensory
learning experience to children. Children explore new experience and
scenarios as new stories are being told. This study concentrates on
determining the best combination of elements for designing effective digital
storytelling applications specifically for the usage of dyslexic children.
Dyslexic children are known to have a common learning difficulty that can
cause problems with reading, writing, spelling and comprehension. These
applications are design with the objective to help in improving dyslexic
children ability in readings and comprehensions. Four elements were
derived from extensive literature studies. The elements are multimedia
components, multi-sensory instructional approach, emotional design and
games design. The relationship among all the elements were determine
and described in details as it will be used to contribute to the design and
development of the application in further works. The strength of this study
is it models the combinations of technology, psychology and instructional
approach as a support components for developing an effective digital story
telling learning application for dyslexic children.
In this study the role of Polymer flooding as one of the most efficient processes to enhance oil
recovery (EOR) is discussed. As we know, Polyhydroxybutyrate (PHB) is a bio-based polymer that
has potential application for use in polymer flooding. This polymer is reviewed with particular
emphasis on the effect of concentration, shear rate, salinity, hardness and temperature on polymer
viscosity. Initial findings showed that PHB owned higher resistant as compared to mechanical
degradation and thermal stability of HPAM as well as XG.
Proteases were extracted from starfruit at maturity Index 2 (unripe, light green) and Index 7 (very ripe, orange) and partially purified using acetone and 40% ammonium sulfate precipitations. Higher yield and proteolytic activity were observed for proteases purified using acetone than 40% ammonium sulfate. As for maturity index, yield and protein concentration of proteases from Index 2 were higher than those from Index 7. SDS-PAGE result showed intense bands for acetone proteases while a distinct band at 50 kDa was observed in all the proteases. Enzyme activity decreased during the seven days storage at 4°C with minimum relative activity of 70% achieved for acetone proteases at day seven. This study suggested that acetone precipitation is more effective method for purifying starfruit protease based on the yield and proteolytic activity compared to using 40% ammonium sulphate precipitation. In order to obtain higher protein concentration and proteolytic activity, starfruit at the unripe stage, Index 2 is a better raw material than Index 7 to be used for protease production.
The purpose of this study was to investigate the effect of papaya leaves crude extract on the physicochemical properties of marinated chicken meat. Papaya leaves was extracted with sodium acetate (CH3COONa) buffer (pH 7.2) at room temperature. Protein concentration and activity of enzyme in the crude extract were determined by using UV-Spectrophotometer. The crude extract was mixed with marinated ingredients and then coated onto chicken meat which was subsequently kept for overnight in refrigerator at chill temperature. Protein concentration of the enzyme was identified as 166.36 μg/μl and enzyme activity was 1.28 CDU/ml. Results also showed that lightness (L*), cooking loss and shrinkage of the marinated chicken were higher than control. Redness (a*), yellowness (b*), protein content, water-holding capacity, shear force, and texture profile analysis were lower than control. Microstructure analysis showed that the chicken meat muscle was destructed in the presence of papaya crude extract. Sensory acceptability evaluation of marinated chicken which was carried out by using 9 point hedonic scale suggested that chicken meat treated with papaya crude extract gave significant effect on texture, juiciness, flavour and overall acceptability compared to commercial bromelain. Additionally, the use of papaya crude extract gave a significant effect on the physicochemical properties of marinated chicken.