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Fulltext Purification and Characterization of Bacteriocin Produced by Weissella confusa A3 of Dairy Origin

Goh HF, Philip K

PLoS One, 2015;10(10):e0140434.
PMID: 26474074 DOI: 10.1371/journal.pone.0140434

A dramatic increase in bacterial resistance towards currently available antibiotics has raised worldwide concerns for public health. Therefore, antimicrobial peptides (AMPs) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases. The present investigation focusses on the isolation and purification of a novel bacteriocin from an indigenous sample of cow milk and it's mode of action. The bacteriocin was isolated from Weissella confusa A3 that was isolated from the sample and was shown to have inhibitory activity towards pathogenic bacteria namely Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Micrococcus luteus. The bacteriocin was shown to be heat stable and functioned well at low pH (2 to 6). Reduction of activity was shown after treatment with proteinase K, trypsin and peptidase that confirmed the proteinaceous nature of the compound. MALDI-TOF analysis of the sample gave a mass approximating 2.7 kDa. The membrane of the bacteria was disrupted by the bacteriocin causing SYTOX® green dye to enter the cell and bind to the bacterial DNA giving fluorescence signal. Bacterial cell treated with the bacteriocin also showed significant morphological changes under transmission electron microscope. No virulence and disease related genes can be detected from the genome of the strain.

Matched MeSH terms: Dairy Products/microbiology*
Fulltext Higher body mass index and lower intake of dairy products predict poor glycaemic control among Type 2 Diabetes patients in Malaysia

Shu PS, Chan YM, Huang SL

PLoS One, 2017;12(2):e0172231.
PMID: 28234927 DOI: 10.1371/journal.pone.0172231

This cross-sectional study was designed to determine factors contributing to glyceamic control in order to provide better understanding of diabetes management among Type 2 Diabetes patients. A pre-tested structured questionnaire was used to obtain information on socio-demographic and medical history. As a proxy measure for glycaemic control, glycosylated haemoglobin (HbA1c) was obtained as secondary data from the medical reports. Perceived self-care barrier on diabetes management, diet knowledge and skills, and diet quality were assessed using pretested instruments. With a response rate of 80.3%, 155 subjects were recruited for the study. Mean HbA1c level of the subjects was 9.02 ± 2.25% with more than 70% not able to achieve acceptable level in accordance to WHO recommendation. Diet quality of the subjects was unsatisfactory especially for vegetables, fruits, fish and legumes as well as from the milk and dairy products group. Higher body mass index (BMI), poorer medication compliance, lower diet knowledge and skill scores and lower intake of milk and dairy products contributed significantly on poor glycaemic control. In conclusion, while perceived self-care barriers and diet quality failed to predict HbA1c, good knowledge and skill ability, together with appropriate BMI and adequate intake of dairy products should be emphasized to optimize glycaemic control among type 2 diabetes patients.

Matched MeSH terms: Dairy Products*
Fulltext High body fat percentage and low consumption of dairy products were associated with vitamin D inadequacy among older women in Malaysia

Leiu KH, Chin YS, Mohd Shariff Z, Arumugam M, Chan YM

PLoS One, 2020;15(2):e0228803.
PMID: 32053636 DOI: 10.1371/journal.pone.0228803

BACKGROUND: Serum vitamin D insufficiency is a public health issue, especially among older women. Sun exposure is fundamental in the production of vitamin D, but older women have less optimal sun exposure. Therefore, factors such as body composition and diet become more essential in sustaining sufficient serum levels of vitamin D. The objective of the current study is to determine factors contributing towards serum vitamin D insufficiency among 214 older women.
METHODS: The respondents had their body weight, height, waist circumference and body fat percentage measured, as well as interviewed for their socio-demographic characteristics, sun exposure and dietary intake. Fasting blood samples were obtained from the respondents to measure their serum 25-hydroxyvitamin D [25(OH)D] concentration.
RESULTS: There were 82.7% (95% CI: 77.6%, 87.8%) of the respondents that had serum vitamin D insufficiency (< 50 nmol/L) with an average of 37.4 ± 14.3nmol/L. In stepwise multiple linear regression, high percentage of body fat (ß = -0.211, p <0.01) and low consumption of milk and dairy products (ß = 0.135, p <0.05) were the main contributors towards insufficient serum vitamin D levels, but not socio-demographic characteristics, other anthropometric indices, sun exposure and diet quality.
CONCLUSION: Older women with high body fat percentage and low dairy product consumption were more likely to have serum vitamin D insufficiency. Older women should ensure their body fat percentage is within a healthy range and consume more milk and dairy products in preventing serum vitamin D insufficiency.

Matched MeSH terms: Dairy Products*
Atititude of asians to calcium and vitamin d rich foods and supplements: a systematic review

Chin Yi Chan, Norazlina Mohamed, Soelaiman Ima-nirwana, Kok-yong Chin

Sains Malaysiana, 2018;47:1801-1810.

The prevalence of osteoporosis is increasing in Asian countries. Optimum calcium intake and vitamin D level are
important to prevent osteoporosis. The attitude of the society towards calcium food sources and supplements, as well
as sun exposure, influence their bone health practices. This review aimed to summarize the current literature on the
attitude of Asians on calcium and vitamin D rich foods and supplements to understand the barriers among the Asians
in achieving adequate calcium intake and vitamin D level. Studies showed that most Asians have a negative attitude
towards calcium-rich foods, mainly dairy products due to lactose intolerance, aversion to the taste of dairy products or
the absence of motivation. Although they took supplements to fill the gap in their diet, they were not aware of the daily
recommended intake of calcium. Besides, the majority of them have a negative attitude towards sun exposure due to the
fear of getting skin cancer or tanning. As a conclusion, most of Asians are still not aware of the importance of calcium
and vitamin D for their health and their negative attitude must be corrected to prevent the rise of osteoporosis.

Matched MeSH terms: Dairy Products
Fulltext CONSUMERS’ KNOWLEDGE, PERCEPTION, AND ATTITUDE TOWARDS NUTRITION AND HEALTH CLAIMS ON DAIRY PRODUCTS AND PURCHASE INTENTION IN JOHOR BAHRU

YAP HUI YEE, SITI NUR'AFIFAH JAAFAR

UMT Journal of Undergraduate Research, 2019;1(2):92-102.
MyJurnal

owadays the increasing prevalence of dietary disease has raised consumers’ awareness of healthy diet and lifestyle. The rise in the use of nutrition and health claims to promote health benefits in food products help consumers make effective food choices. There is very limited research regarding nutrition and health claims in Malaysia. This research focused on consumers’ knowledge, perception (i.e. perceived quality, perceived healthiness, perceived value, perceived importance, trust and perceived familiarity), and attitude towards nutrition and health claims on dairy products as well as their purchase intention towards the products, and examinethe relationship between these variables in Johor Bahru, Johor. All responses were collected by distributing questionnaire to convenience and quota sample (n=200). Data was analysed to obtain descriptive statistics and correlation analysis. The results showed that most of the consumers (46%) had moderate knowledge of nutrition and health claims on dairy products. Consumers generally had almost positive perception and attitude towards nutrition and health claims on dairy products and positive purchase intention towards dairy products. Almost all independent variables correlated with purchase intention towards dairy products. Consumer attitude had the strongest positive relationship with purchase intention (r=0.722), followed by moderate positive correlation between consumer perception and purchase intention (r=0.671), while consumer knowledge only had low relationship with purchase intention (r=0.299). This study added new knowledge regarding public purchasing behaviour towards dairy products with nutrition and health claims. This research also provided valuable information for both food manufacturers and companies to help them make effective decisions to improve the presentation of nutrition and health claims for better expression of information as well as the goodness of food products in order to attract the attention of health-conscious consumers.

Matched MeSH terms: Dairy Products