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Fulltext Entropy, Measures of Distance and Similarity of Q-Neutrosophic Soft Sets and Some Applications

Abu Qamar M, Hassan N

Entropy (Basel), 2018 Sep 05;20(9).
PMID: 33265761 DOI: 10.3390/e20090672

The idea of the Q-neutrosophic soft set emerges from the neutrosophic soft set by upgrading the membership functions to a two-dimensional entity which indicate uncertainty, indeterminacy and falsity. Hence, it is able to deal with two-dimensional inconsistent, imprecise, and indeterminate information appearing in real life situations. In this study, the tools that measure the similarity, distance and the degree of fuzziness of Q-neutrosophic soft sets are presented. The definitions of distance, similarity and measures of entropy are introduced. Some formulas for Q-neutrosophic soft entropy were presented. The known Hamming, Euclidean and their normalized distances are generalized to make them well matched with the idea of Q-neutrosophic soft set. The distance measure is subsequently used to define the measure of similarity. Lastly, we expound three applications of the measures of Q-neutrosophic soft sets by applying entropy and the similarity measure to a medical diagnosis and decision making problems.
The effects of genotypes and media composition on callogenesis, regeneration and cell suspension culture of chamomile (Matricaria chamomilla L.)

Ahmad A, Tahir Ul Qamar M, Shoukat A, Aslam MM, Tariq M, Hakiman M, et al.

PeerJ, 2021;9:e11464.
PMID: 34113490 DOI: 10.7717/peerj.11464

Background: Chamomile is an important herb being used widely for medicinal purposes. Its multitherapeutic, cosmetic, and nutritional values have been established through years of traditional and scientific use and research. Increased use of medicinal plants necessitates rational use as well as sustainable production of such genetic resources. Plant in vitro micro-propagation poses unique opportunities for sustainable production of medicinal herbs, their regrowth and conservation. The present study aimed to investigate the effects of different explants, plant growth regulators (PGRs) combinations and media type on callogenesis, in vitro regeneration and cell suspension of six chamomile genotypes to enhance its sustainable production.
Methods: The shoot, lateral sprout, and leaf derived explants of six chamomile genotypes including Isfahan, Shiraz, Kazeron, Goral, Sharokashari and Presso were used for direct and indirect regeneration. For indirect regeneration various doses of NAA and kinetin were used to induce calli which were cultured on MS media containing PGRs for direct and indirect regeneration. Later, cell suspension was established and morphological characterization of CrO3 stained cells was carried out using microscopy.
Results and Discussion: Our findings revealed that the highest callus percentage and callus volume were observed from lateral sprouts and shoots of genotype Isfahan on MS medium containing 1 mg/L NAA and 1 mg/L kinetin. The in vitro regeneration was found to be genotype dependent while 77% and 77.5% was the highest percentage for indirect and direct regeneration, respectively. Additionally, the maximum shoot number (two shoots/explant) and shoot length (2.22 cm) were also observed in Isfahan genotype. Cell suspension culture showed the highest fresh weight (18.59 g) and dry weight (1.707 g) with 0.75 g inoculum of the callus derived from lateral sprouts cultured on MS medium. Microscopy of CrO3 stained cells was carried on each 3rd day for 27 days that revealed larger and spongier cells in the early days as compared to final days when the cell number was greater but cell size was smaller.
Conclusion: The callogenesis, organogenesis, and cell suspension culture of chamomile may be genotype dependent. Hence, optimization of media ingredients and culture conditions is of utmost importance for devising tissue culture based conservation strategy of any chamomile genotype and secondary metabolite production.
Harnessing bio and (Photo)catalysts for microplastics degradation and remediation in soil environment

Adamu H, Bello U, IbrahimTafida U, Garba ZN, Galadima A, Lawan MM, et al.

J Environ Manage, 2024 Nov;370:122543.
PMID: 39305881 DOI: 10.1016/j.jenvman.2024.122543

Soil pollution by microplastics (MPs) is an escalating environmental crisis with far-reaching consequences. However, current research on the degradation and/or remediation of MPs has mainly focused on water-simulated environments, with little attention given to soil MPs. Therefore, the review explores such terrestrial territory, exploring the potential of biodegradation and novel photocatalytic technologies for MPs degradation/remediation in soil. This review comprehensively investigates the potential of biological and photocatalytic approaches for soil MPs degradation and remediation. A temporal analysis of research from 2004 to 2024 highlights the increasing focus on this critical issue. The review explores the biocatalytic roles of diverse enzymes, including cutinase, PETase, MHETase, hydrolase, lipase, laccase, lignin peroxidase, and Mn-peroxidase, in MPs degradation. Strategies for enzyme engineering, such as protein engineering and immobilization, are explored to enhance catalytic efficiency. The potential for developing enzyme consortia for optimized MP degradation is also discussed. Photocatalytic remediation using TiO2, ZnO, clay, hydrogel, and other photocatalysts is examined, emphasizing their mechanisms and effectiveness. Computational modeling is proposed to deepen understanding of soil MPs-catalyst interactions, primarily aiming to develop novel catalysts tailored for soil environments for environmental safety and sustainable restoration. A comparative analysis of biological and photocatalytic approaches evaluates their environmental implications and the potential for synergistic combinations, with emphasis on soil quality protection, restoration and impact on soil ecosystems. Hence, this review accentuates the urgent need for innovative solutions to address MPs pollution in soil and provides a foundational understanding of the current knowledge gaps, as well as paves the way for future research and development.
Structural probing of HapR to identify potent phytochemicals to control Vibrio cholera through integrated computational approaches

Tahir Ul Qamar M, Ahmad S, Khan A, Mirza MU, Ahmad S, Abro A, et al.

Comput Biol Med, 2021 11;138:104929.
PMID: 34655900 DOI: 10.1016/j.compbiomed.2021.104929

Cholera is a severe small intestine bacterial disease caused by consumption of water and food contaminated with Vibrio cholera. The disease causes watery diarrhea leading to severe dehydration and even death if left untreated. In the past few decades, V. cholerae has emerged as multidrug-resistant enteric pathogen due to its rapid ability to adapt in detrimental environmental conditions. This research study aimed to design inhibitors of a master virulence gene expression regulator, HapR. HapR is critical in regulating the expression of several set of V. cholera virulence genes, quorum-sensing circuits and biofilm formation. A blind docking strategy was employed to infer the natural binding tendency of diverse phytochemicals extracted from medicinal plants by exposing the whole HapR structure to the screening library. Scoring function criteria was applied to prioritize molecules with strong binding affinity (binding energy
Fulltext Multiepitope-Based Subunit Vaccine Design and Evaluation against Respiratory Syncytial Virus Using Reverse Vaccinology Approach

Tahir Ul Qamar M, Shokat Z, Muneer I, Ashfaq UA, Javed H, Anwar F, et al.

Vaccines (Basel), 2020 Jun 08;8(2).
PMID: 32521680 DOI: 10.3390/vaccines8020288

Respiratory syncytial virus (RSV) is primarily associated with respiratory disorders globally. Despite the availability of information, there is still no competitive vaccine available for RSV. Therefore, the present study has been designed to develop a multiepitope-based subunit vaccine (MEV) using a reverse vaccinology approach to curb RSV infections. Briefly, two highly antigenic and conserved proteins of RSV (glycoprotein and fusion protein) were selected and potential epitopes of different categories (B-cell and T-cell) were identified from them. Eminently antigenic and overlapping epitopes, which demonstrated strong associations with their respective human leukocyte antigen (HLA) alleles and depicted collective ~70% coverage of the world's populace, were shortlisted. Finally, 282 amino acids long MEV construct was established by connecting 13 major histocompatibility complex (MHC) class-I with two MHC class-II epitopes with appropriate adjuvant and linkers. Adjuvant and linkers were added to increase the immunogenic stimulation of the MEV. Developed MEV was stable, soluble, non-allergenic, non-toxic, flexible and highly antigenic. Furthermore, molecular docking and molecular dynamics (MD) simulations analyses were carried out. Results have shown a firm and robust binding affinity of MEV with human pathogenic toll-like receptor three (TLR3). The computationally mediated immune response of MEV demonstrated increased interferon-γ production, a significant abundance of immunoglobulin and activation of macrophages which are essential for immune-response against RSV. Moreover, MEV codons were optimized and in silico cloning was performed, to ensure its increased expression. These outcomes proposed that the MEV developed in this study will be a significant candidate against RSV to control and prevent RSV-related disorders if further investigated experimentally.