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Validation of a roughness parameters for defining surface roughness of prosthetic polyethylene Pe-Lite liner

Hamzah NA, Razak NAA, Karim MSA, Salleh SZ

Sci Rep, 2022 Feb 16;12(1):2636.
PMID: 35173198 DOI: 10.1038/s41598-022-05173-3

The Biosculptor's CNC milling machine, the Biomill, offered four different surfaces machined on positive models. This study aims to adopt the surface topography method in characterizing the four different surface roughness of polyethylene Pe-Lite liner as a product of the Biomill. Three surface parameters chosen were the arithmetic average (Ra), root mean square roughness (Rq), and ten-point height (Rz). The surface parameters were used to define the four different surfaces (STANDARD, FINE, COARSE, and FAST) and then compared with the same liner material from a conventionally fabricated socket. The Ra values of the conventional liner, 8.43 μm, were determined to be in-between the Ra values of STANDARD and FAST surfaces which were 8.33 μm and 8.58 μm respectively. STANDARD surface required 43.2 min to be carved while FAST surface took almost only a third of the time compared to STANDARD surface (conventional socket takes 2-3 days). The results of this study would be one of the guidelines to the prosthetists using the Biosculptor in socket fabrication to produce sockets according to the suitable surface to cater to different requirements and levels of activity of each amputee.
Comparative study on the accuracy of Biosculptor's Bioscanner system in transtibial residual limb circumference measurement

Hamzah NA, Razak NAA, Karim MSA, Salleh SZ

Acta Bioeng Biomech, 2021;23(4):173-182.
PMID: 37341100

PURPOSE: This study aimed to examine the accuracy and validity of the Biosculptor's Bioscanner shape capturing system as a portable measuring device by analysing the changes in transtibial residual limb circumference parameters while walking. Assessment on an amputee could also allow for the clinical usability of the digital scanner to be studied.
METHODS: To verify the accuracy of the system, the Bioscanner method was compared to the widely used standard anthropometric manual measurement technique (i.e., tape measure). One transtibial prosthetic user was recruited to conduct a walking activity at a normal walking pace for 5 to 15 minutes. Circumferential profiles of the participant were obtained digitally and manually during 2-5 minutes of resting walking intervals. The mean differences between the two methods were compared and percentage differences were calculated. The means were used to calculate the standard error measurement (SEM) and the 95% confidence intervals. Study of the limit of agreement between the two method was also used to validate the accuracy of Bioscanner.
RESULTS: The findings showed that both measurements gave a general comparable linear pattern. The averaged results from both methods resulted in only small distinctive differences especially at circumference near the mid-patella tendon. Similarly, the pressure-sensitive areas of the limb resulted in only an average of 2.28% differences between the two measurement techniques. The system showed high reliability and SEM with <1 of 95% CI values and repeatability study gave ICC >0.9.
CONCLUSIONS: Bioscanner appeared to be comparable with the standard manual method. The Biosculptor system provides the portability, fast, reliable, and high accuracy measurements of the transtibial residual limb circumference, thus, it can be considered as a valuable tool for daily measurement of amputee's residual limb and pre-prosthetic training.
Potent Antidiabetic Activity and Metabolite Profiling of Melicope Lunu-ankenda Leaves

Al-Zuaidy MH, Hamid AA, Ismail A, Mohamed S, Abdul Razis AF, Mumtaz MW, et al.

J Food Sci, 2016 May;81(5):C1080-90.
PMID: 27074520 DOI: 10.1111/1750-3841.13293

Diabetes mellitus is normally characterized by chronic hyperglycemia associated with disturbances in the fat, carbohydrate, and protein metabolism. There is an increasing trend of using natural products instead of synthetic agents as alternative therapy for disorders due to their fewer side effects. In this study, antidiabetic and antioxidant activities of different Melicope lunu-ankenda (ML) ethanolic extracts were evaluated using inhibition of α-glucosidase and 2,2-diphenyl-l-picrylhydrazyl (DPPH) radicals scavenging activity, respectively; whereas, proton nuclear magnetic resonance ((1) H NMR) and ultra-high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS) techniques were used for metabolite profiling of ML leaf extracts at different concentrations of ethanol and water. Sixty percent of ethanolic ML extract showed highest inhibitory effect against α-glucosidase enzyme (IC50 of 37 μg/mL) and DPPH scavenging activity (IC50 of 48 μg/mL). Antidiabetic effect of ML extracts was also evaluated in vivo and it was found that the high doses (400 mg/Kg BW) of ML extract exhibited high suppression in fasting blood glucose level by 62.75%. The metabolites responsible for variation among ML samples with variable ethanolic levels have been evaluated successfully using (1) H-NMR-based metabolomics. The principal component analysis (PCA) and partial least squares(PLS) analysis scores depicted clear and distinct separations into 4 clusters representing the 4 ethanolic concentrations by PC1 and PC2, with an eigenvalue of 69.9%. Various (1) H-NMR chemical shifts related to the metabolites responsible for sample difference were also ascribed. The main bioactive compounds identified attributing toward the separation included: isorhamnetin, skimmianine, scopoletin, and melicarpinone. Hence, ML may be used as promising medicinal plant for the development of new functional foods, new generation antidiabetic drugs, as a single entity phytomedicine or in combinational therapy.
Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats

Abdul Majid N, Abdul Hamid A, Salleh SZ, Saari N, Abas F, Pak Dek MS, et al.

Phytochem Anal, 2020 Mar;31(2):191-203.
PMID: 31381209 DOI: 10.1002/pca.2880

INTRODUCTION: Natural products are obtaining much acceptance as ergogenic aid, not only among athletes but also among the general population including people with excess body fat. Under normal circumstances, an obese person will have the desire and ability to exercise reduced; mainly because they are easily fatigued. Thus, they need to boost their energy production so that they can be more active and healthier.
OBJECTIVE: In this present work, Morinda citrifolia L. leaf extract (MLE) which is believed to possess ergogenic property, was evaluated on its effect on an obese animal model using 1 H-NMR based metabolomics.
MATERIAL AND METHODS: Rats were fed with high fat diet (HFD) for 12 weeks for obese development. Once this was achieved, all the rats underwent endurance exercise (forced swimming test) every 2 weeks for 8 weeks together with treatment. The time to exhaustion was recorded for each rat. Three different dosages of MLE: 50 mg/kg, 100 mg/kg and 200 mg/kg of body weight were used together with two positive controls: 5 mg/kg caffeine and 100 mg/kg green tea. Blood was collected before and after treatments for metabolomics study.
RESULTS: Findings showed that feeding the rats at a dose of 200 mg/kg body weight MLE significantly prolonged the exhaustive swimming time of the rats, and altered the metabolites present in their serum. Discriminating metabolites involved were the product of various metabolic pathways, including carbohydrate, lipids metabolism and energy metabolism. Treatment with 200 mg/kg body weight MLE resulted in significant improvement in the metabolic perturbations where the proximity of the obese exercised treated group to that of normal exercised group in the partial least squares discriminant analysis score plot was observed.
CONCLUSION: The present work demonstrated ergogenic property of MLE based on the improved metabolic perturbation in exercised obese rats.
Ergogenic property of Morinda citrifolia L. leaf extract affects energy metabolism in obese Sprague Dawley rats

Salleh SZ, Hamid AA, Jaafar AH, Abdul Majid ND, Saari N, Halim HH, et al.

J Food Biochem, 2022 Jan;46(1):e14027.
PMID: 34914111 DOI: 10.1111/jfbc.14027

Ergogenic property is the ability to enhance capacity for physical activities through efficient production of energy and is potentially beneficial in weight management for the obese. In this study, ergogenic property of Morinda citrifolia leaf's extract (MCL) was evaluated using AMP-activated protein kinase (AMPK) activity and high fat diet-induced obese rats. Findings from the study showed that MCL demonstrated ergogenic activity via enhancement of AMPK activity using L6 skeletal muscle cell line. Interestingly, the result also revealed that rats treated with the intermediate dosage of MCL experienced the lowest % weight gain. The rats fed the highest dose of 200 mg/kg BW MCL demonstrated the longest swimming time of approximately three times that of green tea and caffeine-fed rats. The highest dose fed rats were also found to have lower glucose and lactate levels, suggesting that energy metabolism was more effective in these rats. In addition, lactate dehydrogenase and creatinine kinase activities, the muscle injury indicators, were found to be the lowest in rats fed the highest MCL dose. The same effect was not seen in rats fed either caffeine or green tea, indicating that MCL treatment is may be protective of the rats' muscles. It was also shown that MCL consisted of various flavonoids with epicatechin, catechin, and quercetin that may be responsible for the effects measured. In conclusion, improvements were seen in rats fed MCL in terms of weight management, endurance capacity, energy metabolism, and muscle injury parameters. PRACTICAL APPLICATIONS: Results of the study revealed that Morinda citrifolia leaf has great potential to be used as functional ingredient in the development of designer food/drink as ergogenic aid for both obese and non-obese individuals. Morinda citrifolia leaf could help in the weight management of obese people and enhance endurance capacity and energy metabolism in active individuals.