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Caregiver coping with the mentally ill: a qualitative study

Azman A, Jamir Singh PS, Sulaiman J

J Ment Health, 2017 Apr;26(2):98-103.
PMID: 26732363 DOI: 10.3109/09638237.2015.1124395

BACKGROUND: Mental illness is a disease that affects millions of people every year. It not only causes stress to the mentally ill patients, but also for the family members who provide them the care. The family caregivers, therefore need some form of coping strategies in dealing with their mentally ill family members.
AIMS: This qualitative study aims at identifying and analysing the coping strategies adopted by the family caregivers in dealing with their mentally ill family members.
METHOD: A total of 15 family caregivers from the state of Kedah, Malaysia participated in the face-to-face semi structured interview.
RESULTS: The study findings identified an array of coping strategies used by the family caregivers, including religious coping, emotional coping, acceptance, becoming engaged in leisure activities, and the use of traditional healing to help them cope with their mentally ill members. Suggestions and conclusions: Study suggests that the family caregivers should engage themselves in social support groups to learn about and obtain the positive coping strategies used by other caregivers who have similar experiences in caring for the mentally ill. Study also suggests that they should get appropriate training from the mental health professionals in order to enhance the caregivers' coping skills.
Fulltext Microbial succession and the functional potential during the fermentation of Chinese soy sauce brine

Sulaiman J, Gan HM, Yin WF, Chan KG

Front Microbiol, 2014;5:556.
PMID: 25400624 DOI: 10.3389/fmicb.2014.00556

The quality of traditional Chinese soy sauce is determined by microbial communities and their inter-related metabolic roles in the fermentation tank. In this study, traditional Chinese soy sauce brine samples were obtained periodically to monitor the transitions of the microbial population and functional properties during the 6 months of fermentation process. Whole genome shotgun method revealed that the fermentation brine was dominated by the bacterial genus Weissella and later dominated by the fungal genus Candida. Metabolic reconstruction of the metagenome sequences demonstrated a characteristic profile of heterotrophic fermentation of proteins and carbohydrates. This was supported by the detection of ethanol with stable decrease of pH values. To the best of our knowledge, this is the first study that explores the temporal changes in microbial successions over a period of 6 months, through metagenome shotgun sequencing in traditional Chinese soy sauce fermentation and the biological processes therein.
Fulltext Rhodotorula mucilaginosa, a quorum quenching yeast exhibiting lactonase activity isolated from a tropical shoreline

Ghani NA, Sulaiman J, Ismail Z, Chan XY, Yin WF, Chan KG

Sensors (Basel), 2014;14(4):6463-73.
PMID: 24721765 DOI: 10.3390/s140406463

Two microbial isolates from a Malaysian shoreline were found to be capable of degrading N-acylhomoserine lactones. Both Matrix Assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry and 18S rDNA phylogenetic analyses confirmed that these isolates are Rhodotorula mucilaginosa. Quorum quenching activities were detected by a series of bioassays and rapid resolution liquid chromatography analysis. The isolates were able to degrade various quorum sensing molecules namely N-hexanoyl-L-homoserine lactone (C6-HSL), N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) and N-(3-hydroxyhexanoyl)-L-homoserine lactone (3-hydroxy-C6-HSL). Using a relactonisation assay to verify the quorum quenching mechanism, it is confirmed that Rh. mucilaginosa degrades the quorum sensing molecules via lactonase activity. To the best of our knowledge, this is the first documentation of the fact that Rh. mucilaginosa has activity against a broad range of AHLs namely C6-HSL, 3-oxo-C6-HSL and 3-hydroxy-C6-HSL.
Fulltext Draft Genome Perspective of Staphylococcus saprophyticus Strain SU8, an N-Acyl Homoserine Lactone-Degrading Bacterium

Chan KG, Sulaiman J, Yong DA, Tee KK, Yin WF, Priya K

Genome Announc, 2015;3(5).
PMID: 26404582 DOI: 10.1128/genomeA.01097-15

Staphylococcus saprophyticus strain SU8 was isolated from a pristine water source in Malaysia and it exhibited degradation of N-hexanoylhomoserine lactone. Here we report the draft genome sequence of S. saprophyticus strain SU8 to further understand its quorum quenching abilities.
Production of N-acyl homoserine lactones by Chromobacterium haemolyticum KM2 isolated from the river water in Malaysia

Priya K, Sulaiman J, How KY, Yin WF, Chan KG

Arch Microbiol, 2018 Sep;200(7):1135-1142.
PMID: 29796703 DOI: 10.1007/s00203-018-1526-y

Quorum sensing (QS) is a term used to describe cell-to-cell communication that enables bacteria to orchestrate group behaviours according to density of bacterial cells. In Gram-negative bacteria, this signalling system is widely known to regulate a variety of different phenotypes such as antibiotic production and biofilm formation. In this study, we report the production of N-acyl homoserine lactones produced by Chromobacterium haemolyticum strain KM2, a bacterium isolated from a river water of a reserved tropical national park. Preliminary screening of QS activity using biosensor reporter assays indicated that C. haemolyticum strain KM2 produces both short- and long-chain AHLs. Analysis with high-resolution liquid chromatography-mass spectrometry (LC-MS/MS) analysis revealed the production of three AHLs by strain KM2: N-octanoyl-L-homoserine lactone (C8-HSL), N-dodecanoyl-L-homoserine lactone (C12-HSL), and N-3-oxo-dodecanoyl-L-homoserine lactone (OC12-HSL). This bacterial isolate also exhibited strong β-haemolytic activity. To the best of our knowledge, this is the first documentation of QS activity and multiple AHLs production by C. haemolyticum strain KM2.
Fulltext An automated materials and processes identification tool for material informatics using deep learning approach

Miah MSU, Sulaiman J, Sarwar TB, Ibrahim N, Masuduzzaman M, Jose R

Heliyon, 2023 Sep;9(9):e20003.
PMID: 37809409 DOI: 10.1016/j.heliyon.2023.e20003

This article reports a tool that enables Materials Informatics, termed as MatRec, via a deep learning approach. The tool captures data, makes appropriate domain suggestions, extracts various entities such as materials and processes, and helps to establish entity-value relationships. This tool uses keyword extraction, a document similarity index to suggest relevant documents, and a deep learning approach employing Bi-LSTM for entity extraction. For example, materials and processes for electrical charge storage under an electric double layer capacitor (EDLC) mechanism are demonstrated herewith. A knowledge graph approach finds and visualizes different latent knowledge sets from the processed information. The MatRec received an F1 score of 9̃6% for entity extraction, 8̃3% for material-value relationship extraction, and 8̃7% for process-value relationship extraction, respectively. The proposed MatRec could be extended to solve material selection issues for various applications and could be an excellent tool for academia and industry.
Fulltext Quorum sensing activity of Enterobacter asburiae isolated from lettuce leaves

Lau YY, Sulaiman J, Chen JW, Yin WF, Chan KG

Sensors (Basel), 2013 Oct 22;13(10):14189-99.
PMID: 24152877 DOI: 10.3390/s131014189

Bacterial communication or quorum sensing (QS) is achieved via sensing of QS signaling molecules consisting of oligopeptides in Gram-positive bacteria and N-acyl homoserine lactones (AHL) in most Gram-negative bacteria. In this study, Enterobacteriaceae isolates from Batavia lettuce were screened for AHL production. Enterobacter asburiae, identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was found to produce short chain AHLs. High resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) analysis of the E. asburiae spent supernatant confirmed the production of N-butanoyl homoserine lactone (C4-HSL) and N-hexanoyl homoserine lactone (C6-HSL). To the best of our knowledge, this is the first report of AHL production by E. asburiae.