Hymenocallis littoralis (Jacq.) Salisb (Melong kecil) commonly known as 'Spider Lily' is an herbaceous plant from the family Amaryllidaceae. Study was carried out to determine the effect of H. littoralis leaf extract on the growth and morphogenesis of two pathogenic microbes, Candida albicans and Escherichia coli. The leaf extract displayed favourable anticandidal and antibacterial activity with a minimum inhibition concentration (MIC) of 6.25 mg/mL. Time kill study showed both microbes were completely killed after treated with leaf extract at 20 h. Both microbes' cell walls were heavily ruptured based on scanning electron microscopy (SEM) analysis. The significant anticandidal and antibacterial activities showed by H. littoralis leaf extract suggested the potential antimicrobial agent against C. albicans and E. coli.
Some synthetic preservatives have become controversial because they have been proven to cause health problems. These increased health concerns have led consumers to prefer food preservatives based on natural products. Hence, Stenochlaena palustris leaf extract was used in this study to evaluate the antifungal activity against food borne pathogen, Aspergillus niger. The value of minimum inhibitory concentration and minimum fungicidal concentration of leaf extract for this fungus grown on Potato Dextrose Agar medium was 50 mg/ml. IC50 value for the hyphal growth of A. niger was at a concentration of 17.41 mg/ml. Morphology changes of A. niger treated with the fern leaf extract was observed through scanning electron microscope. The thread-like and elongated hyphae cell wall was disrupted, with some appearing flattened and others being broken. Currently, there is growing interest in using natural food preservatives such as medicinal plant extracts for preserving foods to reduce outbreaks of foodborne pathogenic microorganisms. Hence, S. palustris appears to have promise as a safe alternative natural product-based food preservative for future generations.
Cassia (C.) surattensis Burm. f. (Leguminosae), a medicinal herb native to tropical equatorial Asia, was commonly used in folk medicine to treat various diseases. The aim of the present study is to investigate the effects of methanolic flower extract of C. surattensis against Aspergillus (A.) niger.
Invasive aspergillosis (IA) in immunocompromised host is a major infectious disease leading to reduce the survival rate of world population. Aspergillus niger is a causative agent causing IA. Cassia surattensis plant is commonly used in rural areas to treat various types of disease. C. surattensis flower extract was evaluated against the systemic aspergillosis model in this study. Qualitative measurement of fungal burden suggested a reduction pattern in the colony forming unit (CFU) of lung, liver, spleen and kidney for the extract treated group. Galactomannan assay assessment showed a decrease of fungal load in the treatment and positive control group with galactomannan index (GMI) value of 1.27 and 0.25 on day 28 but the negative control group showed high level of galactomannan in the serum with GMI value of 3.58. Histopathology examinations of the tissues featured major architecture modifications in the tissues of negative control group. Tissue reparation and recovery from infection were detected in extract treated and positive control group. Time killing fungicidal study of A. niger revealed dependence of the concentration of C. surattensis flower extract.