MATERIALS AND METHODS: UV-visible spectra analysis was employed to investigate the optical characteristics and surface morphology, such as the size and shape of PPE CaSo4 NPs synthesized at different time intervals, which were characterized using a Scanning Electron Microscope. Anti-inflammatory activity was evaluated using bovine serum albumin denaturation assay (BSA), and egg albumin denaturation assay (EA) was compared with diclofenac sodium as a standard. Antioxidant activity was measured using 2,2 Diphenyl -1- Picryl hydraxylhydrate assay (DPPH), hydrogen peroxide radical scavenging assay (H2O2), and Ferric Reducing Antioxidant power assay (FRAP). Comparison made with ascorbic acid as the standard.

RESULTS: Anti-inflammatory activity was observed at all concentrations of PPE CaSo4 NPs, and there was no significant difference between the test material and the standard p>0.05. A significant difference was found for the antioxidant activity between PPE CaSo4 NPs and the standard in concentrations of 10 μl, for DPPH, 10 μl and 20 μl for H2O2 (p<0.05) between the concentrations of 30, 40, and 50, and there was no significant difference between the test material and the standard in all three tests conducted.

MATERIALS AND METHODS: The antioxidant activity was tested by Hydrogen Peroxide [H2O2] assay, Fluorescence recovery after photo-bleaching [FRAP] assay and 2, 2-diphenyl-1- picrylhydrazyl[DPPH] assay. All tests have shown very good results for the ZnO-TiO2 NCs.

RESULTS: In this study, we present a straightforward, ecofriendly alternative for producing non-toxic zinc oxide and titanium oxide nano-composite material. This study could make a valuable contribution and create new opportunities in the market such as biological and pharmaceutical applications.