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  1. Aghwan ZA, Sazili AQ, Alimon AR, Goh YM, Hilmi M
    Asian-Australas J Anim Sci, 2013 Nov;26(11):1577-82.
    PMID: 25049744 DOI: 10.5713/ajas.2013.13180
    The effects of dietary supplementation of selenium (Se), iodine (I), and a combination of both on the blood haematology, serum free thyroxine (FT4) and free triiodothyronine (FT3) hormones and glutathione peroxidase enzyme (GSH-Px) activity were examined on twenty four (7 to 8 months old, 22±1.17 kg live weight) Kacang crossbred male goats. Animals were randomly assigned to four dietary treatments (6 animals in each group). Throughout 100 d of feeding trial, the animals of control group (CON) received a basal diet, while the other three groups were offered basal diet supplemented with 0.6 mg/kg diet DM Se (SS), or 0.6 mg/kg diet DM I (PI), or a combination of both Se and I, each at 0.6 mg/kg diet DM (SSPI). The haematological attributes which are haemoglobin (Hb), red blood cell (RBC), packed cell volume (PCV), mean cell volume (MCV), white blood cells (WBC), band neutrophils (B Neut), segmented neutrophils (S Neut), lymphocytes (Lymph), monocytes (Mono), eosinophils (Eosin) and basophils (Baso) were similar among the four treatment groups, while serum levels of Se and I increased significantly (p<0.05) in the supplemented groups. The combined dietary supplementation of Se and I (SSPI) significantly increased serum FT3 in the supplemented animals. Serum GSH-Px activity increased significantly in the animals of SS and SSPI groups. It is concluded that the dietary supplementation of inorganic Se and I at a level of 0.6 mg/kg DM increased serum Se and I concentration, FT3 hormone and GSH-Px activity of Kacang crossbred male goats.
    Matched MeSH terms: Basophils
  2. Abdulla MA, Khairul Anuar A, Khalifa S, Salmah I, Mohamad Nazmul HM, Suzainur KAR, et al.
    JUMMEC, 2002;7:135-141.
    Four calves from each group of purebred Kedah kelantan (KK), halfbred KK X Friesian, and quarterbred KK X Friesian were experimentally infested with Boophilus micropills larvae. Two calves from each genotype were injected, intramuscularly with antihistamine while the remaining two animals in each genotype received the same dose of antihistamine and dexamethasone. Dexamethasone combined anti-histamine treatment suppress tick resistance as manifested by the production of higher number of engorged female ticks, higher mean weight of replete ticks, mean weight of eggs and mean number of larvae hatched from 1 g of eggs. In anti-histamine treated animals there was no reduction of resistant in all animals as manifested by a few ticks were able to feed successfully. At 1, 2, and 3 hours post-larval attachment in anti-histamine and dexamethasone treated cattle there was complete ablation of the cellular infiltration in the dermis beneath the tick mouthparts, especially eosinophil and basophils. There was little destruction of tissue. However, in anti-histamine treated cattle there were more cellular infiltration and degranulation in the dermis. The cells infiltrating the dermis were mainly eosinophils followed by neutrophs, mast cells and basophils and some of these cells showed sign of degranulation. At 24 hours postlarval attachment, animals lTeated with anti-histamine and dexamethasone showed reduction of, cellular infiltration, degranulation, size of the epidermal lesion and tissue damage. The neutrophils were the predominant cells within the epidermal lesions. However, animals in anti-histamine treatment showed edema, more cellular infiltration and degranulation, and destruction of tissues as compared to antihistamine and dexamethasone treated animals. In anti-histamine treated cattle the epidermal lesions were obviously larger and the percentage of eosinophils and basophils were higher than those of antihistamine and dexamethasone treated animals. KEYWORDS: Kedah-Kelantan cattle, KK X Friesian callie, B. micropflls, dexamethasone, antihistamine, cellular response.
    Matched MeSH terms: Basophils
  3. Odhah MN, Abdullah FFJ, Haron AW, Lila MAM, Zamri-Saad M, Khuder Z, et al.
    Vet World, 2017 Jun;10(6):655-661.
    PMID: 28717318 DOI: 10.14202/vetworld.2017.655-661
    AIM: This study was conducted to analyze the changes in blood profile of goats inoculated with Corynebacterium pseudotuberculosis and its immunogen mycolic acid (MA) extract.

    MATERIALS AND METHODS: A total of 12 clinically healthy crossbred Boer female goats were divided into three groups; A, B and C (4 goats each per group). Group A was inoculated with 2 ml sterile phosphate buffered saline via intradermal route as the negative control group whilst Group B was inoculated with 2 ml of MA extract (1 g/ml) intradermally and Group C was then inoculated with 2 ml (1×10(9)) colony forming unit of active C. pseudotuberculosis intradermally. Blood sample was collected aseptically from the jugular vein periodically for complete blood count (CBC) analysis throughout the experimental period (3 months).

    RESULT: A significant decrease (p<0.05) was observed in red blood cells, hemoglobin (Hb), packed cell volume, mean corpuscular volume and mean corpuscular Hb concentration in Groups B and C as compared to the control while WBCs, neutrophil, lymphocyte and basophil showed a significant increase (p<0.05) as compared to the control.

    CONCLUSION: The inoculation of C. pseudotuberculosis and MA resulted in a significant change in the CBC, thereby, indicating that MA has a role in caseous lymphadenitis pathogenesis.

    Matched MeSH terms: Basophils
  4. Leecyous B, Bakhtiar F, Tang MM, Yadzir ZHM, Abdullah N
    Allergol Immunopathol (Madr), 2020 06 09;48(6):626-632.
    PMID: 32532468 DOI: 10.1016/j.aller.2020.01.006
    INTRODUCTION: Basophil activation test (BAT) and immunoassays are the most widely used in vitro tests to diagnose IgE-mediated allergic reactions to penicillin. However, studies to determine if one test is interdependent from another are limited.

    OBJECTIVE: The present study aimed to measure the agreement between BAT and immunoassay in diagnosis of penicillin allergy.

    METHOD: BAT was performed using penicillin G (Pen G), penicillin V (Pen V), penicilloyl-polylysine (PPL), minor determinant mix (MDM), amoxicillin (Amx) and ampicillin (Amp) in 25 patients. Immunoassay of total IgE (tIgE) and specific IgE (sIgE) antibodies to Pen G, Pen V, Amx and Amp were quantified. Skin prick test (SPT) using PPL-MDM, Amx, Amp and Clavulanic acid were also performed.

    RESULTS: Minimal agreement was observed between BAT and immunoassay (k=0.25). Of two BAT-positive patients, one patient is positive to Amx (59.27%, SI=59) and Amp (82.32%, SI=82) but sIgE-negative to all drug tested. This patient is also SPT-positive to both drugs. Another patient is BAT-positive to Pen G (10.18%, SI=40), Pen V (25.07%, SI=100) and Amp (19.52%, SI=79). In sIgE immunoassay, four patients were sIgE-positive to at least one of the drugs tested. The sIgE level of three patients was between low and moderate and they were BAT-negative. One BAT-positive patient had a high level of sIgE antibodies (3.50-17.5kU/L) along with relatively high specific to total IgE ratio ≥0.002 (0.004-0.007).

    CONCLUSIONS: The agreement between BAT and immunoassay is minimal. Performing both tests provides little increase in the sensitivity of allergy diagnosis work-up for immediate reactions to penicillin.

    Matched MeSH terms: Basophils/immunology
  5. Odhaib KJ, Adeyemi KD, Ahmed MA, Jahromi MF, Jusoh S, Samsudin AA, et al.
    Trop Anim Health Prod, 2018 Jun;50(5):1011-1023.
    PMID: 29654500 DOI: 10.1007/s11250-018-1525-7
    The objective of this study was to determine the effects of dietary supplementation of Nigella sativa L. seeds, Rosmarinus officinalis L. leaves and their combination on rumen metabolism, nutrient intake and digestibility, growth performance, immune response and blood metabolites in Dorper lambs. Twenty-four entire male Dorper lambs (18.68 ± 0.6 kg, 4-5 months old) were randomly assigned to a concentrate mixture containing on a dry matter basis either, no supplement (control, T1), 1% R. officinalis leaves (T2), 1% N. sativa seeds (T3) or 1% R. officinalis leaves +1% N. sativa seeds (T4). The lambs had ad libitum access to urea-treated rice straw (UTRS) and were raised for 90 days. Supplemented lambs had greater (P 
    Matched MeSH terms: Basophils/drug effects
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