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Fulltext Performance comparison of EasyFix G26 and HYDRASYS 2 SCAN for the detection of serum monoclonal proteins

Amin Nordin FD, Mohd Khalid MKN, Abdul Aziz SM, Mohamad Bakri NA, Ahmad Ridzuan SN, Abdul Jalil J, et al.

J Clin Lab Anal, 2020 Jun;34(6):e23254.

BACKGROUND: Serum protein electrophoresis (SPE) is a widely used laboratory technique to diagnose patients with multiple myeloma (MM) and other disorders related to serum protein. In patients with MM, abnormal monoclonal protein can be detected by SPE and further characterized using immunofixation electrophoresis (IFE). There are several semi-automated agarose gel-based systems available commercially for SPE and IFE. In this study, we sought to evaluate the analytical performance of fully automated EasyFix G26 (EFG26) and semi-automated HYDRASYS 2 SCAN (H2SCAN) for both SPE and IFE.

METHODS: Both instruments were operated according to manufacturer's instructions. Samples used include a commercially available normal control serum (NCS) and patients' specimens. The following were evaluated: precision and comparison studies for SPE, and reproducibility and comparison studies for IFE. Statistical analyses were performed using Microsoft Excel.

RESULTS: For SPE repeatability study, our results showed that EFG26 has higher coefficient of variation (%CV) compared with H2SCAN for both samples except for monoclonal component with %CV of 0.97% and 1.18%, respectively. Similar results were obtained for SPE reproducibility study except for alpha-1 (4.16%) and beta (3.13%) fractions for NCS, and beta fractions (5.36%) for monoclonal sample. Subsequently, reproducibility for IFE was 100% for both instruments. Values for correlation coefficients between both instruments ranged from 0.91 to 0.98 for the five classic bands.

CONCLUSION: Both instruments demonstrated good analytical performance characterized by high precision, reproducibility and correlation.

Matched MeSH terms: Blood Protein Electrophoresis/instrumentation*; Blood Protein Electrophoresis/methods

Serum free light chains: diagnostic and prognostic value in multiple myeloma

Sthaneshwar P, Nadarajan V, Maniam JA, Nordin N, Gin Gin G

Clin Chem Lab Med, 2009;47(9):1101-7.

PMID: 19728852 DOI: 10.1515/CCLM.2009.260

Measurement of serum free light chains (FLCs) has recently become available for the diagnosis and monitoring of patients with plasma cell dyscrasias. The aim of this study was to investigate the performance of the serum FLC assay as a tumour marker by comparing FLC concentrations with serum protein electrophoresis (PE) results in the diagnosis of multiple myeloma (MM). In addition, we also evaluated the prognostic value of the baseline serum FLC ratio in patients with MM.

Matched MeSH terms: Blood Protein Electrophoresis/methods*

Serum albumin variants in three Malaysian racial groups

Welch QB, Lie-Injo LE

Hum Hered, 1972;22(5):503-7.

PMID: 4670071

Matched MeSH terms: Blood Protein Electrophoresis

TROPHOBLASTIC TUMORS; GEOGRAPHICAL VARIATIONS IN INCIDENCE AND POSSIBLE AETIOLOGICAL FACTORS

LLEWELLYN-JONES D

J Obstet Gynaecol Br Commonw, 1965 Apr;72:242-8.

PMID: 14273103

Matched MeSH terms: Blood Protein Electrophoresis*

Fulltext Haemoglobin Lepore in a Malay family: a case report

Pasangna J, George E, Nagaratnam M

Malays J Pathol, 2005 Jun;27(1):33-7.

PMID: 16676691

A 2-year-old Malay boy was brought to the University Malaya Medical Centre for thalassaemia screening. Physical examination revealed thalassaemia facies, pallor, mild jaundice, hepatomegaly and splenomegaly. Laboratory investigations on the patient including studies on the parents lead to a presumptive diagnosis of homozygous Haemoglobin Lepore (Hb Lepore). The aim of this paper is to increase awareness of this rare disorder, this being the first case documented in Malaysia in a Malay. The case also demonstrates the need for this disorder to be included in the differential diagnosis of patients presenting clinically like thalassemia intermedia or thalassemia major. Accurate diagnosis would provide information necessary for prenatal diagnosis, proper clinical management and genetic counseling. The clinical, haematological and laboratory features of this disorder are discussed in this paper.

Matched MeSH terms: Blood Protein Electrophoresis

Protein variation and systematics of three subgenera of Malayan rats (Rodentia: Muridae, genus Rattus Fischer)

Chan KL, Dhaliwal SS, Yong HS

Comp. Biochem. Physiol., B, 1979;64(4):329-37.

PMID: 318313

1. Nine erythrocyte proteins coded by a separate locus each were analysed in and among seven Malayan species of Rattus belonging to three subgenera. 2. Electrophoretic data obtained confirm the specific status of the seven taxa and divide the seven species into three groups which correspond with Ellerman's (1949) subgenera Stenomys, Maxomys and Leopoldamys. 3. A comparative study together with 11 other species of Malayan Rattus previously analysed show that, with few exceptions, the overall relationships among the 18 species based on electrophoretic data correspond well with conclusions based on morphological evidence. 4. Malayan species of Rattus are relatively very diverse genetically (S = 0.27, range 0.01-0.94).

Matched MeSH terms: Blood Protein Electrophoresis

Erythrocyte and leukocyte phosphoglucomutase in Chinese

Lie-Injo Luan Eng, Lopez CG, Poey-Oey Hoey Giok

Am J Hum Genet, 1968 Mar;20(2):101-6.

PMID: 5643177

Matched MeSH terms: Blood Protein Electrophoresis

Haemoglobin F Malaysia: alpha 2, gamma 2 1(NA1) glycine--cysteine; 136 glycine

Eng LI, Kamuzora H, Lehmann H

J Med Genet, 1974 Mar;11(1):25-30.

PMID: 4837284

Matched MeSH terms: Blood Protein Electrophoresis

Haemoglobin E variants and pregnancy in Malaysian aborigines

Ong HC

Acta Haematol., 1974;52(4):220-2.

PMID: 4217527 DOI: 10.1159/000208244

Haemoglobin E complicates 22.2°/o of pregnancy in Malaysian aborigines, the prevalence of variants associated with pregnancy being, 15.8% with Hb E trait abnormality, 3.9% with Hb E homozygous disease, and 2.5% with Hb E thalassaemia disease. Minor haematological abnormalities occur with the trait and homozygous conditions, though a more unfavourable response is expected with Hb E thalassaemia. Haemolysis is not a prominent feature and it is suggested that factors other than the haemoglobinopathic state
probably accounts for any unfavourable response in pregnancy.
Key Words: Haemoglobin E; Haemoglobinopathies; Haemolytic anaemias; Hb E thalassaemia; Malaysia; Pregnancy
Study site: Hospital Orang Asli, Gombak, Selangor, Malaysia

Matched MeSH terms: Blood Protein Electrophoresis

Phosphoglucomutase and carbonic anhydrase in West Malaysian aborigines

Welch QB, Lie-Injo Luan Eng, Bolton JM

Hum Hered, 1972;22(1):28-37.

PMID: 4624781

Matched MeSH terms: Blood Protein Electrophoresis

Human red cell adenylate kinase polymorphism in West Malaysian populations

Chan KL

Hum Hered, 1971;21(2):173-9.

PMID: 5127408

Matched MeSH terms: Blood Protein Electrophoresis

Unusual albumin variants in Indonesians and Malayan aborigines

Lie-Injo Luan Eng, Weitkamp LR, Kosasih EN, Bolton JM, Moore CL

Hum Hered, 1971;21(4):376-83.

PMID: 5003129

Matched MeSH terms: Blood Protein Electrophoresis

Homozygous state for Hb Constant Spring (slow-moving Hb X components)

Lie-Injo LE, Ganesan J, Clegg JB, Weatherall DJ

Blood, 1974 Feb;43(2):251-9.

PMID: 4810076

Matched MeSH terms: Blood Protein Electrophoresis

Inheritance of haemoglobin H disease. A new aspect

Lie-Injo LE, Lopez CG, Lopes M

Acta Haematol., 1971;46(2):106-20.

PMID: 4331171 DOI: 10.1159/000208565

A study of 23 patients with Hb H disease and their 82 relatives in 17 families showed that 2 types of this condition exist. One is associated with the presence of a small slow-moving component, which we tentatively called the X component and which was invariably present in one parent. Some siblings also had it. The other type was not associated with this component. Two patients without X component had a newborn with Bart’s haemoglobin without X component. None of the parents of 20 newborns with Hb Bart’s without the X component had the X component. It was present in only one parent of each of 2 newborns with Hb Bart’s and the X component. They are thought to represent Hb H disease in the newborn period. We suggest that at least 3 abnormal genes may lead to Hb H disease, which results when 2 of the 3 combine. Severity of clinical and haematological symptoms depends upon which abnormal gene is present and which 2 are involved in any particular combination.
Key Words: a-Thalassaemia; Haemoglobin Bart’s; Haemoglobin H disease; Haemoglobinopathies

Matched MeSH terms: Blood Protein Electrophoresis

Structural identification of haemoglobin F Kuala Lumpur: alpha2 gamma2 22(B4)Asp leads to Gly; 136 Ala

Luan Eng LI, Wiltshire BG, Lehmann H

Biochim. Biophys. Acta, 1973 Oct 18;322(2):224-30.

PMID: 4765089

Matched MeSH terms: Blood Protein Electrophoresis

Genetic red cell abnormalities in Trengganu and Perlis (West Malaysia)

Eng LI, McKay DA, Govindasamy S

Southeast Asian J Trop Med Public Health, 1971 Jun;2(2):133-9.

PMID: 5002823

Matched MeSH terms: Blood Protein Electrophoresis

Alpha-thalassaemia in newborns in West Malaysia

Lopez CG, Lie-Injo Luan Eng

Hum Hered, 1971;21(2):185-91.

PMID: 5127409

Matched MeSH terms: Blood Protein Electrophoresis

Mild beta-thalassemia intermedia caused by compound heterozygosity for (G)gamma((A)gammadeltabeta)(o)/beta-thalassemia and molecular characterization of the defect in four Chinese families

Tan JAMA, Yap SF, Tan KL, Wong YC, Wee YC, Kok JL

Acta Haematol., 2003;109(4):169-75.

PMID: 12853688 DOI: 10.1159/000070965

Molecular characterization of the compound heterozygous condition - (G)gamma((A)gammadeltabeta)(o)/beta-thalassemia - in four families showing mild beta-thalassemia intermedia was carried out using DNA amplification techniques. Using the Amplification Refractory Mutation System (ARMS) to confirm the beta-mutations and DNA amplification to detect the 100-kb Chinese-specific (G)gamma((A)gammadeltabeta)(o)-deletion, ()two families were confirmed to possess (G)gamma((A)gammadeltabeta)(o)/beta-thalassemia with the IVSII No. 654 beta(+)-allele. In the third family, the (G)gamma((A)gammadeltabeta)(o)-deletion was confirmed in the father and the mother was a beta-thalassemia carrier with the cd 41-42 beta(o)-allele. Their affected child with (G)gamma((A)gammadeltabeta)(o)/beta-thalassemia was found to be transfusion dependent. The same (G)gamma((A)gammadeltabeta)(o)-deletion and beta-thalassemia (cd 41-42) was also confirmed in a fourth family. In addition, the mother was also diagnosed with Hb H disease (genotype -alpha(3.7)/-(SEA)). Both the children were found to possess (G)gamma((A)gammadeltabeta)(o)/beta-thalassemia but they were not transfusion dependent and this could be due to co-inheritance of alpha-thalassemia-2 (genotype-alpha(3.7)/alphaalpha) in the children together with their compound heterozygous condition.

Matched MeSH terms: Blood Protein Electrophoresis

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