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Efficiency of PV/T collector using spiral thermal absorber design

Cheku Nurul Aisyah, Ahmad Fudholi, Mohd Yusof Othman, Adnan Ibrahim, Mohd Hafidz Ruslan, Kamaruzzaman Sopian

Sains Malaysiana, 2018;47:853-859.

Kajian ini bertujuan untuk mereka bentuk dan mengkaji prestasi pengumpul fotovoltan-terma (PV/T) dengan menggunakan
reka bentuk pengumpul terma aliran air secara pilin. Reka bentuk pengumpul terma aliran air secara pilin telah dibina
semula daripada reka bentuk penyelidik sebelum ini. Pengumpul terma ini menggunakan saiz tiub yang lebih besar dan
jarak antara tiub lebih dekat berbanding dengan reka bentuk penyelidik sebelum ini. Pengumpul PV/T ini telah diuji di
bawah tiga keamatan yang berbeza iaitu 300, 500 dan 700 W/m2 serta diuji pada kadar aliran jisim air yang berbeza
iaitu 0.01, 0.02 dan 0.04 kg/s. Pada keadaan yang sama, kecekapan fotovoltan, terma dan tergabung juga menunjukkan
nilai yang paling tinggi, iaitu 4.18%, 76.68% dan 80.86%. Kecekapan terma yang dihasilkan pada kajian ini lebih tinggi
dibandingkan dengan kajian sebelumnya.

Matched MeSH terms: Fimbriae Proteins
Fulltext Twitching motility of Stenotrophomonas maltophilia under iron limitation: In-silico, phenotypic and proteomic approaches

V K, Neela VK

Virulence, 2020 Dec;11(1):104-112.
PMID: 31957553 DOI: 10.1080/21505594.2020.1713649

This study investigates the twitching ability of 28 clinical and five environmental strains of S. maltophilia grown under iron-depleted condition through in-silico, phenotypic and proteomics approaches. Rapid Annotations using Subsystem Technology (RAST) analysis revealed the presence of 21 targets of type IV pilus shared across S. maltophilia strains K279a, R551-3, D457 and JV3. The macroscopic twitching assay showed that only clinical isolates produced a zone of twitching with a mean of 22.00 mm under normal and 25.00 mm under iron-depleted conditions. (p = 0.002). Environmental isolates did not show any significant twitching activity in both conditions tested. Isobaric Tags for Relative and Absolute Quantification (ITRAQ) analysis showed altered expression of twitching motility protein PilT (99.08-fold change), flagellar biosynthesis protein FliC (20.14-fold change), and fimbrial protein (0.70-fold change) in response to iron-depleted condition. Most of the strains that have the ability to twitch under the normal condition, exhibit enhanced twitching during iron limitation.

Matched MeSH terms: Fimbriae Proteins/metabolism
Fulltext Colonisation factors amongst clinical isolates of enterotoxigenic Escherichia coli

Iyer L, Vadivelu J, Parasakthi N

Singapore Med J, 1995 Oct;36(5):495-7.
PMID: 8882532

The production of heat-labile (LT) and heat-stable (ST) enterotoxins, colonisation factor antigens (CFAs) and haemagglutinins was investigated amongst 310 Escherichia coli (E. coli) isolates obtained from 62 children under the age of five, with diarrhoea. Twenty-one isolates were found to produce enterotoxins, of which fifteen (71%) isolates produced ST only, 2 (10%) produced LT only and 4 (19%) produced both LT and ST. However, none of the isolates demonstrated any of the common CFAs identified to date, but 8 out of the 21 isolates demonstrated haemagglutination with rabbit, sheep or human group A erythrocytes, suggesting the presence of putative CFAs, yet unidentified.

Matched MeSH terms: Fimbriae Proteins*
Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats

Mohd Yasin IS, Mohd Yusoff S, Mohd ZS, Abd Wahid Mohd E

Trop Anim Health Prod, 2011 Jan;43(1):179-87.
PMID: 20697957 DOI: 10.1007/s11250-010-9672-5

This study was carried out to determine the antibody responses and protective capacity of an inactivated recombinant vaccine expressing the fimbrial protein of Pasteurella multocida B:2 following intranasal vaccination against hemorrhagic septicemia in goats. Goats were vaccinated intranasal with 10(6) CFU/mL of the recombinant vaccine (vaccinated group) and 10(6) CFU/mL of pET32/LIC vector without fimbrial protein (control group). All three groups were kept separated before all goats in the three groups were challenged with 10(9) CFU/mL of live pathogenic P. multocida B:2. During the course of study, both serum and lung lavage fluid were collected to evaluate the antibody levels via enzyme-linked immunosorbent assay. It was found that goats immunized with the inactivated recombinant vaccine developed a strong and significantly (p

Matched MeSH terms: Fimbriae Proteins/genetics; Fimbriae Proteins/immunology*
Fulltext Imipenem Treatment Induces Expression of Important Genes and Phenotypes in a Resistant Acinetobacter baumannii Isolate

Dhabaan GN, AbuBakar S, Cerqueira GM, Al-Haroni M, Pang SP, Hassan H

Antimicrob Agents Chemother, 2015 Dec 14;60(3):1370-6.
PMID: 26666943 DOI: 10.1128/AAC.01696-15

Acinetobacter baumannii has emerged as a notorious multidrug-resistant pathogen, and development of novel control measures is of the utmost importance. Understanding the factors that play a role in drug resistance may contribute to the identification of novel therapeutic targets. Pili are essential for A. baumannii adherence to and biofilm formation on abiotic surfaces as well as virulence. In the present study, we found that biofilm formation was significantly induced in an imipenem-resistant (Imp(r)) strain treated with a subinhibitory concentration of antibiotic compared to that in an untreated control and an imipenem-susceptible (Imp(s)) isolate. Using microarray and quantitative PCR analyses, we observed that several genes responsible for the synthesis of type IV pili were significantly upregulated in the Imp(r) but not in the Imp(s) isolate. Notably, this finding is corroborated by an increase in the motility of the Imp(r) strain. Our results suggest that the ability to overproduce colonization factors in response to imipenem treatment confers biological advantage to A. baumannii and may contribute to clinical success.

Matched MeSH terms: Fimbriae Proteins/genetics
In vitro assessment of pathogenicity and virulence encoding gene profiles of avian pathogenic Escherichia coli strains associated with colibacillosis in chickens

Ugwu IC, Lee-Ching L, Ugwu CC, Okoye JOA, Chah KF

Iran J Vet Res, 2020;21(3):180-187.
PMID: 33178295

Background: Avian pathogenic Escherichia coli (APEC) strains have been associated with various disease conditions in avian species due to virulence attributes associated with the organism.
Aims: This study was carried out to determine the in vitro pathogenic characteristics and virulence encoding genes found in E. coli strains associated with colibacillosis in chickens.
Methods: Fifty-two stock cultures of E. coli strains isolated from chickens diagnosed of colibacillosis were tested for their ability to produce haemolysis on blood agar and take up Congo red dye. Molecular characterization was carried out by polymerase chain reaction (PCR) amplification of virulence encoding genes associated with APEC.
Results: Eleven (22%) and 41 (71%) were positive for haemolysis on 5% sheep red blood agar and Congo red agar, respectively. Nine virulence-associated genes were detected as follows: FimH (96%), csgA (52%), iss (48%), iut (33%), tsh (21%), cva (15%), kpsII (10%), pap (2%), and felA (2%).
Conclusion: The APEC strains exhibited virulence properties and harbored virulence encoding genes which could be a threat to the poultry population and public health. The putative virulence genes were diverse and different in almost all isolate implying that pathogenesis was multi-factorial and the infection was multi-faceted which could be a source of concern in the detection and control of APEC infections.

Matched MeSH terms: Fimbriae Proteins