MyMedR

Displaying all 8 publications

Abstract:

Sort:

Ultrasound-assisted phosphorylation of goose myofibrillar proteins: improving protein structure and functional properties

Zhou Y, Sun Y, Pan D, Xia Q, Zhou C

J Sci Food Agric, 2023 Aug 30;103(11):5412-5421.
PMID: 37038882 DOI: 10.1002/jsfa.12616

BACKGROUND: Goose meat is rough and embedded with dense connective tissue, impairing protein solubility. Therefore, to improve the functional properties of goose myofibrillar protein (GMP), ultrasound was used to assist the phosphorylation of GMP.
RESULTS: The fact that GMP attached covalently with the phosphate group of sodium tripolyphosphate (GMP-STP) was disclosed directly by Fourier transform infrared spectroscopy. Furthermore, ultrasound significantly improved the hydrophobicity and solubility of GMP-STP, which could be attributed to the conversion of α-helix to β-sheet, β-turns, and random coils by sonication. The spatial stabilization of the protein phosphorylation process was boosted by ultrasound, making the droplets more dispersed, and thus an improvement in the functional properties of GMP-STP was observed. Water-holding capacity, oil-binding capacity, and emulsifying and foaming properties were best at an ultrasound power of 400 W.
CONCLUSION: Ultrasound-assisted phosphorylation has great potential to modulate the structure-function relationship of proteins. © 2023 Society of Chemical Industry.

Matched MeSH terms: Geese*
Fulltext Seroprevalence of avian influenza in Baltic common eiders (Somateria mollissima) and pink-footed geese (Anser brachyrhynchus)

Lam SS, Tjørnløv RS, Therkildsen OR, Christensen TK, Madsen J, Daugaard-Petersen T, et al.

Environ Int, 2020 09;142:105873.
PMID: 32585505 DOI: 10.1016/j.envint.2020.105873

Blood plasma was collected during 2016-2018 from healthy incubating eiders (Somateria molissima, n = 183) in three Danish colonies, and healthy migrating pink-footed geese (Anser brachyrhynchus, n = 427) at their spring roost in Central Norway (Svalbard breeding population) and their novel flyway through the Finnish Baltic Sea (Russian breeding population). These species and flyways altogether represent terrestrial, brackish and marine ecosystems spanning from the Western to the Eastern and Northern part of the Baltic Sea. Plasma of these species was analysed for seroprevalence of specific avian influenza A (AI) antibodies to obtain information on circulating AI serotypes and exposure. Overall, antibody prevalence was 55% for the eiders and 47% for the pink-footed geese. Of AI-antibody seropositive birds, 12% (22/183) of the eiders and 3% (12/427) of the pink-footed geese had been exposed to AI of the potentially zoonotic serotypes H5 and/or H7 virus. AI seropositive samples selected at random (n = 33) showed a low frequency of serotypes H1, H6 and H9. Future projects should aim at sampling and isolating AI virus to characterize dominant serotypes and virus strains (PCR). This will increase our understanding of how AI exposure may affect health, breeding and population viability of Baltic common eiders and pink-footed geese as well as the potential spill-over to humans (zoonotic potential).

Matched MeSH terms: Geese*
A novel Tembusu virus isolated from goslings in China form a new subgenotype 2.1.1

Zhu Y, Hu Z, Lv X, Huang R, Gu X, Zhang C, et al.

Transbound Emerg Dis, 2022 Jul;69(4):1782-1793.
PMID: 33993639 DOI: 10.1111/tbed.14155

Since 2010, several duck Tembusu viruses (DTMUVs) have been isolated from infected ducks in China, and these virus strains have undergone extensive variation over the years. Although the infection rate is high, the mortality rate is usually relatively low-~5%-30%; however, since fall 2019, an infectious disease similar to DTMUV infection but with a high mortality rate of ~50% in goslings has been prevalent in Anhui Province, China. The present study identified a new Tembusu virus, designated DTMUV/Goose/China/2019/AQ-19 (AQ-19), that is believed to be responsible for the noticeably high mortality in goslings. To investigate the genetic variation of this strain, its entire genome was sequenced and analysed for specific variations, and goslings and mice were challenged with the isolated virus to investigate its pathogenicity. The AQ-19 genome shared only 94.3%-96.9% and 90.9% nucleotide identity with other Chinese and Malaysian DTMUVs, respectively; however, AQ-19 has high homology with Thailand DTMUVs (97.2%-98.1% nucleotide identity). Phylogenetic analysis of the E gene revealed that AQ-19 and most of Thailand DTMUVs form a branch separate from any of the previously reported DTMUV strains in China. After the challenge, some goslings and mice showed typical clinical signs of DTMUV, particularly severe neurological dysfunction. AQ-19 has high virulence in goslings and mice, resulting in 60% and 70% mortality through intramuscular and intracerebral routes, respectively. Pathological examination revealed severe histological lesions in the brain and liver of the infected goslings and mice. Taken together, these results demonstrated the emergence of a novel Tembusu virus with high virulence circulating in goslings in China for the first time, and our findings highlight the high genetic diversity of DTMUVs in China. Further study of the pathogenicity and host range of this novel Tembusu virus is particularly important.

Matched MeSH terms: Geese
Fulltext A novel goose-origin Tembusu virus exhibits pathogenicity in day-old chicks with evidence of direct contact transmission

Liu M, Chen YY, Twu NC, Wu MC, Fang ZS, Dubruel A, et al.

Poult Sci, 2024 Feb;103(2):103332.
PMID: 38128459 DOI: 10.1016/j.psj.2023.103332

In late 2020, an outbreak of Tembusu virus (TMUV)-associated disease occurred in a 45-day-old white Roman geese flock in Taiwan. Here, we present the identification and isolation of a novel goose-origin TMUV strain designated as NTU/C225/2020. The virus was successfully isolated using minimal-pathogen-free duck embryos. Phylogenetic analysis of the polyprotein gene showed that NTU/C225/2020 clustered together with the earliest isolates from Malaysia and was most closely related to the first Taiwanese TMUV strain, TP1906. Genomic analysis revealed significant amino acid variations among TMUV isolates in NS1 and NS2A protein regions. In the present study, we characterized the NTU/C225/2020 culture in duck embryos, chicken embryos, primary duck embryonated fibroblasts, and DF-1 cells. All host systems were susceptible to NTU/C225/2020 infection, with observable lesions. In addition, animal experiments showed that the intramuscular inoculation of NTU/C225/2020 resulted in growth retardation and hyperthermia in day-old chicks. Gross lesions in the infected chicks included hepatomegaly, hyperemic thymus, and splenomegaly. Viral loads and histopathological damage were displayed in various tissues of both inoculated and naïve co-housed chicks, confirming the direct chick-to-chick contact transmission of TMUV. This is the first in vivo study of a local TMUV strain in Taiwan. Our findings provide essential information for TMUV propagation and suggest a potential risk of disease outbreak in chicken populations.

Matched MeSH terms: Geese
Pathogenicity of Mycoplasma imitans in mixed infection with infectious bronchitis virus in chickens

Ganapathy K, Bradbury JM

Avian Pathol, 1999 Jun;28(3):229-37.
PMID: 26915378 DOI: 10.1080/03079459994713

Mycoplasma imitans (Mim) has been isolated from ducks, geese and partridges, and is closely related to Mycoplasma gallisepticum (Mg). The pathogenicity of Mim for chicks was investigated in single and mixed infections with infectious bronchitis virus (IBV) by giving IBV strain M41 at 1-day-old and Mim 2 days later. Single infections with IBV or Mim were also performed. No clinical signs or gross lesions were seen in chicks infected with Mim or uninfected control chicks, but they were seen in the other two groups. Clinical scores were consistently higher in birds with mixed infections than those infected with IBV alone, and were significantly higher (P < 0.05) between days 7 and 14. More birds developed sinusitis, tracheitis and airsacculitis (with greater severity) in the mixed than the single IBV infections. Mim was recovered more frequently and in greater numbers from the respiratory tract of birds with mixed than single infections. It was recovered from the lower trachea, air sacs and lungs only in mixed infections. Seroconversion to Mim occurred by day 14 in mixed infections, but not until day 21 in single infections. It appears that Mim can act synergistically with IBV in young chickens in a similar manner to Mg, although Mg may act as a primary pathogen under some circumstances.

Matched MeSH terms: Geese
Infrequent occurrence of vancomycin-resistant enterococci in poultry from Malaysian wet markets

Ong CH, Asaad M, Lim KC, Ngeow YF

Malays J Pathol, 2002 Dec;24(2):91-4.
PMID: 12887166

Fifty samples of chicken, duck and geese faeces were obtained from 13 wet markets in Kuala Lumpur to study the prevalence of vancomycin-resistant enterococci (VRE) among local market poultry. Biotyping of colonies grown on azide agar incubated at 45 degrees C yielded E. pseudoavium, E. faecalis, E. faecium and E. gallinarum from chicken faeces and E. malodoratus, E. faecalis, E. faecium, E. gallinarum, E. hirae/dispar, and E. durans from goose and duck faeces. On agar containing 6 mg/ l of vancomycin, one strain of E. flavescens was identified, giving a VRE detection rate of 2.0%. This isolate had a vancomycin M.I.C. of 8 mg/l as determined by the Etest, and the van C-3 gene that was identified by PCR followed by sequence analysis. The prevalence of VRE among poultry sold in local markets appears to be low, and may reflect the infrequent use of antimicrobials in our poultry farms. Nevertheless, the possibility of human acquisition of microbes via the food chain cautions against the use of antimicrobials in animal husbandry that may encourage the emergence and spread of multi-drug resistant organisms like the VRE among animal microbial flora.

Matched MeSH terms: Geese
Fulltext Prevalence and antimicrobial resistance of Listeria, Salmonella, and Yersinia species isolates in ducks and geese

Jamali H, Radmehr B, Ismail S

Poult Sci, 2014 Apr;93(4):1023-30.
PMID: 24706981 DOI: 10.3382/ps.2013-03699

The aims of this study were to determine the prevalence and antimicrobial resistance of Listeria, Salmonella, and Yersinia spp. isolated from duck and goose intestinal contents. A total of 471 samples, including 291 duck and 180 goose intestinal contents, were purchased from wet markets between November 2008 and July 2010. Listeria, Salmonella, and Yersinia spp. were isolated from 58 (12.3%), 107 (22.7%), and 80 (17%) of the samples, respectively. It was concluded that Listeria ivanovii, Salmonella Thompson, and Yersinia enterocolitica were the predominant serovars among Listeria, Salmonella, and Yersinia spp., respectively. Moreover, resistance to tetracycline was common in Listeria (48.3%) and Salmonella spp. (63.6%), whereas 51.3% of the Yersinia spp. isolates were resistant to cephalothin. Therefore, continued surveillance of the prevalence of the pathogens and also of emerging antibiotic resistance is needed to render possible the recognition of foods that may represent risks and also ensure the effective treatment of listeriosis, salmonellosis, and yersiniosis.

Matched MeSH terms: Geese/microbiology
Japanese encephalitis in Sarawak: virus isolation and serology in a Land Dyak village

Simpson DI, Bowen ET, Platt GS, Way H, Smith CE, Peto S, et al.

Trans R Soc Trop Med Hyg, 1970;64(4):503-10.
PMID: 4394986
Matched MeSH terms: Geese