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  1. Fulltext Synthesis and Characterization of Diosgenin Encapsulated Poly-ε-Caprolactone-Pluronic Nanoparticles and Its Effect on Brain Cancer Cells
    Rabha B, Bharadwaj KK, Baishya D, Sarkar T, Edinur HA, Pati S
    Polymers (Basel), 2021 Apr 18;13(8).
    PMID: 33919483 DOI: 10.3390/polym13081322
    Diosgenin encapsulated PCL-Pluronic nanoparticles (PCL-F68-D-NPs) were developed using the nanoprecipitation method to improve performance in brain cancer (glioblastoma) therapy. The nanoparticles were characterized by dynamic light scattering (DLS)/Zeta potential, Fourier-transform infrared (FTIR) spectra, X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), and Transmission electron microscopy (TEM). The encapsulation efficiency, loading efficiency, and yield were calculated. The in vitro release rate was determined, and the kinetic model of diosgenin release was plotted and ascertained. The cytotoxicity was checked by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide)assay against U87-MG cells (glioblastoma cell lines). The obtained nanoparticles demonstrated good size distribution, stability, morphology, chemical, and mechanical properties. The nanoparticles also possessed high encapsulation efficiency, loading efficiency, and yield. The release rate of Diosgenin was shown in a sustained manner. The in vitro cytotoxicity of PCL-F68-D-NPs showed higher toxicity against U87-MG cells than free Diosgenin.
    Matched MeSH terms: Diosgenin
  2. Fulltext Quantification of anti-fertility compound-diosgenin concentration in the fenugreek seeds aqueous extract (FSA)
    Ahmed Kaid, N. A., Norbaiyah, M. B., Imad, M. A., Norazian, M. H.
    International Medical Journal Malaysia, 2016;15(1):75-80.
    MyJurnal
    Introduction: This study aims to build a standardization method for preparation of effective powder from
    FSA and to quantify diosgenin in FSA. Methodology: One kg of FS were used in this study. Setting: BMS, KOM
    and KOP, IIUM Kuantan campus. FS were washed with distilled water to exclude any foreign matter, and
    were then air dried. FS-powder were put in distilled water in a ratio of 1 g of powder in 20 ml of distilled
    water and were shaken at room temperature for 24 hours. Ten mg of hydrolyzed extract sample was diluted
    in 10 ml volumetric flask with methanol for 15 minutes. Chromatographic estimation was performed using
    an equilibrated reverse phase Eclipse XDB-C18 column (particle size 5 µg, 4.6 mm x 150 mm). Results: One
    gram of FSA extract was hydrolyzed to produce sapogenins and 46.6% was recovered. A calibration curve
    that was constructed based on five dilutions of diosgenin standard at concentrations of 2, 5, 10, 20, 30 and
    50 ppm produced a linear graft (r = 0.999). The concentration of diosgenin in FSA extract as calculated using
    the regression analysis was found to be 29.66 µg/ml, 13.81 % w/w on dried weight basis. Conclusion:
    Preparation and standardization of effective powder from FSA are the corner stone of many scientific
    researches in IIUM and Malaysia. Diosgenin is available in the FSA in adequate concentration. The adequate
    amount of diosgenin in the FSA will guide us to do further study in the way of preparation of a natural
    product that can be used in the field of reversible anti-fertility therapy.
    Matched MeSH terms: Diosgenin
  3. Diosgenin, a steroidal saponin, and its analogs: Effective therapies against different chronic diseases
    Parama D, Boruah M, Yachna K, Rana V, Banik K, Harsha C, et al.
    Life Sci, 2020 Nov 01;260:118182.
    PMID: 32781063 DOI: 10.1016/j.lfs.2020.118182
    BACKGROUND: Chronic diseases are a major cause of mortality worldwide, and despite the recent development in treatment modalities, synthetic drugs have continued to show toxic side effects and development of chemoresistance, thereby limiting their application. The use of phytochemicals has gained attention as they show minimal side effects. Diosgenin is one such phytochemical which has gained importance for its efficacy against the life-threatening diseases, such as cardiovascular diseases, cancer, nervous system disorders, asthma, arthritis, diabetes, and many more.

    AIM: To evaluate the literature available on the potential of diosgenin and its analogs in modulating different molecular targets leading to the prevention and treatment of chronic diseases.

    METHOD: A detailed literature search has been carried out on PubMed for gathering information related to the sources, biosynthesis, physicochemical properties, biological activities, pharmacokinetics, bioavailability and toxicity of diosgenin and its analogs.

    KEY FINDINGS: The literature search resulted in many in vitro, in vivo and clinical trials that reported the efficacy of diosgenin and its analogs in modulating important molecular targets and signaling pathways such as PI3K/AKT/mTOR, JAK/STAT, NF-κB, MAPK, etc., which play a crucial role in the development of most of the diseases. Reports have also revealed the safety of the compound and the adaptation of nanotechnological approaches for enhancing its bioavailability and pharmacokinetic properties.

    SIGNIFICANCE: Thus, the review summarizes the efficacy of diosgenin and its analogs for developing as a potent drug against several chronic diseases.

    Matched MeSH terms: Diosgenin
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