Browse publications by year: 2001

  1. Green SK, Tsai WS, Shih SL, Black LL, Rezaian A, Rashid MH, et al.
    Plant Dis., 2001 Dec;85(12):1286.
    PMID: 30831796 DOI: 10.1094/PDIS.2001.85.12.1286A
    Production of tomato (Lycopersicon esculentum) in Bangladesh, Malaysia, Myanmar, Vietnam, and Laos has been severely affected by yellow leaf curl disease. Tomato leaf samples were collected from symptomatic tomato plants from farmers' fields in the five countries from 1997 to 1999. DNA was extracted from all samples, four from Vietnam, two each from Malaysia, Laos, and Myanmar, and seven from Bangladesh. Virus DNA was amplified by polymerase chain reaction (PCR) using the begomovirus-specific degenerate primer pair PAL1v 1978/PAR1c 715(1), which amplifies the top part of DNA A. All samples gave the expected 1.4-kb PCR product. The PCR product of one sample per country was cloned and sequenced. Based on the sequences of the 1.4-kb DNA products amplified by the first primer pair, specific primers were designed to complete each of the DNA A sequences. Computer-assisted sequence comparisons were performed with begomovirus sequences available in the laboratory at the Asian Vegetable Research and Development Center, Shanhua, Tainan, and in the GenBank sequence database. The five DNA species resembled DNA A of begomoviruses. For the detection of DNA B two degenerate primer pairs were used, DNABLC1/DNABLV2 and DNABLC2/DNABLV2 (DNABLC1: 5'-GTVAATGGRGTDCACTTCTG-3', DNABLC2: 5'-RGTDCACTT CTGYARGATGC-3', DNABLV2: 5'-GAGTAGTAGTGBAKGTTGCA-3'), which were specifically designed to amplify DNA B of Asian tomato geminiviruses. Only the virus associated with yellow leaf curl of tomato in Bangladesh was found to contain a DNA B component, which was detected with the DNABLC1/DNABLV2 primer pair. The DNA A sequence derived from the virus associated with tomato yellow leaf curl from Myanmar (GenBank Accession No. AF206674) showed highest sequence identity (94%) with tomato yellow leaf curl virus from Thailand (GenBank Accession No. X63015), suggesting that it is a closely related strain of this virus. The other four viruses were distinct begomoviruses, because their sequences shared less than 90% identity with known begomoviruses of tomato or other crops. The sequence derived from the virus associated with tomato yellow leaf curl from Vietnam (GenBank Accession No. AF264063) showed highest sequence identity (82%) with the virus associated with chili leaf curl from Malaysia (GenBank Accession No. AF414287), whereas the virus associated with yellow leaf curl symptoms in tomato in Bangladesh (GenBank Accession No. AF188481) had the highest sequence identity (88%) with a tobacco geminivirus from Yunnan, China (GenBank Accession No. AF240675). The sequence derived from the virus associated with tomato yellow leaf curl from Laos (GenBank Accession No. AF195782) had the highest sequence identity (88%) with the tomato begomovirus from Malaysia (GenBank Accession No. AF327436). This report provides further evidence of the great genetic diversity of tomato-infecting begomoviruses in Asia. Reference: M. R. Rojas et al. Plant Dis. 77:340, 1993.
    MeSH terms: Farmers; Bangladesh; Myanmar; China; DNA Viruses; DNA, Viral; Laos; Malaysia; Thailand; Tobacco; Genetic Variation; Vegetables; Vietnam; Polymerase Chain Reaction; DNA Primers; Lycopersicon esculentum; Databases, Nucleic Acid; Begomovirus
  2. Ooi CG, Hew FL
    Family Physician, 2001;11:13-16.
    Overweight and obese adults are at increased risk of both mortality and morbidity, principally from cardiovascular disease and diabetes mellitus. Intentional weight loss of 5-1 0% is associated with substantial benefits. Weight reduction should be encouraged by dietary and behavioural modification initially, with realistic targets set over an initial 6-month period. It must be emphasised that these measures should continue indefinitely. This can be complemented by pharmacological intervention with orlistat, or perhaps sibutramine when available in the near future. Given the important impact environmental and social factors have on the aetiology of obesity, the community at large should effect programmes to encourage healthy lifestyles among the populace. Town and city planners should include readily available recreational and sporting facilities in residential districts, especially in the urban and sub-urban areas. Physical education sessions in schools in both primary and secondary levels should receive more emphasis to encourage the young to make sports and physical exercise a part of life, rather than being seen as a non examinable appendage of the school curriculum. The key to defeating obesity is prevention, and an effort to lose weight once it accumulates, can often be too little, too late.
    MeSH terms: Obesity
  3. Ong CC
    Family Physician, 2001;11:27-29.
    Otitis media is a common disease presenting to family practitioners all over the world. There have been many changes in the way these patients were managed over the years. This article attempts to provide an overview to family physicians regarding the latest approach in managing this common condition. Keywords: Otitis, otitis media, otoscopy, eardrops.
    MeSH terms: Head; London; Malaysia; Neck; Otitis Media; Physicians; Physicians, Family; Seasons; Otoscopy
  4. Loh KY
    Family Physician, 2001;11:37-38.
    MeSH terms: Humans; Malaysia; Terminal Care
  5. Lim KH
    Family Physician, 2001;11:35-36.
    Despite time, mobility, knowledge and other constraints, it is still possible for General Practitioners to play an active role in Palliative Care. This article offers various roles where GP can play. Differences between hospice, palliative medicine, palliative care are discussed. Suggestions are made on where to seek formal or informal education on palliative care. Key Words: role, hospice, palliative medicine, palliative care, illness, sickness
    MeSH terms: Malaysia; Palliative Care; Physicians; Physicians, Family; General Practitioners
  6. Lachmanan SR
    Family Physician, 2001;11:32-33.
    MeSH terms: Hepatitis; Hepatitis B; Malaysia
  7. Lachmanan SR
    Family Physician, 2001;11:30-31.
    MeSH terms: Hepatitis; Hepatitis B; Malaysia; Hepatitis B, Chronic
  8. Kwa SK, Lu AIC, Zairul Azwan MA, Aman Fuad Y, Siti Aishah A
    Family Physician, 2001;11(3):7-10.
    Adolescent pregnancy is associated with long term medical and sociological problems. For intervention, it is important to have information on their profile and obstetric outcome. A study was conducted in 1999 on teenage mothers compared to mothers in the 20-34 year age group. Antenatal records of all these mothers registered in a Malaysian semi-rural Health Clinic in 1998 were reviewed and the relevant information was analysed using descriptive statistics and chi-square for comparison in SPSS 7.5. Only 402 (80.9%) of the 497 antenatal records could be included. There were 40 (9.95%) adolescent pregnancies and 362 (90.05%) pregnancies in mothers aged 20-34 years. Pregnant adolescents were more likely to be Malays (85% versus 66%), unmarried (65% versus 5.5%) and less educated (32.5% versus 12.1%). They have a significantly lower rate of contraceptive usage (2.5% versus 20.2%) and tend to come late for their first antenatal visit (55% versus 18.5%). Their pregnancy complications of anaemia and pregnancy induced hypertension were no worse. But they had a significantly higher preterm delivery rate (37.5% versus 21.8%) and their babies were more likely to have low birth weight (32.5% versus 9.9%). Based on this preliminary finding, further investigations should be carried out and polices should include programmes targeted for this group.
    MeSH terms: Adolescent; Ambulatory Care Facilities; Anemia; Birth Weight; Humans; Obstetric Labor, Premature; Malaysia; Malaysia/ethnology; Mothers; Outpatients; Physicians, Family; Pregnancy; Pregnancy Complications; Pregnancy in Adolescence; Retrospective Studies; Prevalence; Case-Control Studies
  9. Kwa SK
    Family Physician, 2001;11:20-3.
    Asthma is a chronic reversible respiratory problem commonly seen by Family Physicians. Pregnancy can produce physiological and physical changes that can affect the severity of asthma and its management. Conversely poorly controlled asthmatic attacks can result in adverse obstetric outcome: prematurity, low birth weight babies, foetal hypoxia and increased maternal and foetal morbidity and mortality. It is important that Family Physicians should be able to manage competently and provide appropriate counseling for women with asthma in pregnancy, labour and lactation. Assessment of asthma control using a peak flow meter is recommended. The use of the usual inhaled and oral corticosteroids, beta 2 agonists, cromones and anticholinergics are generally safe in pregnancy, labour and lactation. But methylxanthines should be used with caution. Women should be advised that asthma medication would not adversely affect their unborn babies and the birth outcome of well-controlled asthmatic women approaches that of the normal population but uncontrolled asthma would be detrimental to the health of mother and child.
    MeSH terms: Asthma; Humans; Pregnancy
  10. Kwa SK
    Family Physician, 2001;11:39.
    MeSH terms: Coitus; Humans; Malaysia; HIV Infections; Knowledge
  11. Kwa SK
    Family Physician, 2001;11:i-ii.
    MeSH terms: Family Practice
  12. Khairani O
    Family Physician, 2001;11:11-12.
    MeSH terms: Breast Feeding; Malaysia; Mothers; Primary Health Care; Review
  13. Gendeh BS, Sani A
    Family Physician, 2001;11:24-26.
    Epistaxis is a common clinical condition and perhaps the most common ENT cause for emergency hospital admission. Commonly epistaxis originates from the anterior septum and is easily controlled with caurterization and packing. Posterior epistaxis is less common, but more difficult to treat. It usually occurs in the elderly and is frequently associated with hypertension, artherosclerosis and conditions that decrease platelets and clotting function. In the elderly and hypertensive patient the source of bleeding is likely from the sphenopalatine area in lateral posterior nasal wall. Intractable posterior epistaxis that persists despite repeated use of nasal packing has been treated in many different ways. Conventional methods of arterial ligation in intractable epistaxis often involve surgical morbidity as well as failure due to arterial anastomosis. Embolization is most effective in hands of an experience radiologist, in patients with epistaxis refractory to arterial ligation, bleeding site difficult to reach surgically or epistaxis due to general bleeding disorder. Endoscopic ligation or clipping of sphenopalatine artery is a relatively simple and effective procedure for control of intractable posterior epistaxis.
    MeSH terms: Emergencies; Epistaxis; Malaysia
  14. Ednin H
    Family Physician, 2001;11:34-34.
    MeSH terms: Malaysia; Palliative Care
  15. Doshi HH
    Family Physician, 2001;11:40-40.
    MeSH terms: Dermatology; Hepatitis; Hepatitis B; Humans; Wales
  16. Chia YC, Goh KL
    Family Physician, 2001;11:17-19.
    MeSH terms: Malaysia; Primary Health Care; Review; Irritable Bowel Syndrome
  17. Chatenet M, Delage C, Ripolles M, Irey M, Lockhart BEL, Rott P
    Plant Dis., 2001 Nov;85(11):1177-1180.
    PMID: 30823163 DOI: 10.1094/PDIS.2001.85.11.1177
    Sugarcane yellow leaf virus (SCYLV) was detected for the first time in 1996 in the Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) sugarcane quarantine at Montpellier by reverse transcription-polymerase chain reaction (RT-PCR) in varieties from Brazil, Florida, Mauritius, and Réunion. Between 1997 and 2000, the virus was found by RT-PCR and/or tissue-blot immunoassay (TBIA) in additional varieties from Barbados, Cuba, Guadeloupe, Indonesia, Malaysia, Philippines, Puerto Rico, and Taiwan, suggesting a worldwide distribution of the pathogen. An excellent correlation was observed between results obtained for the two diagnostic techniques. However, even though only a few false negative results were obtained by either technique, both are now used to detect SCYLV in CIRAD's sugarcane quarantine in Montpellier. The pathogen was detected by TBIA or RT-PCR in all leaves of sugarcane foliage, but the highest percentage of infected vascular bundles was found in the top leaves. The long hot water treatment (soaking of cuttings in water at 25°C for 2 days and then at 50°C for 3 h) was ineffective in eliminating SCYLV from infected plants. Sugarcane varieties from various origins were grown in vitro by apical bud culture and apical meristem culture, and the latter proved to be the most effective method for producing SCYLV-free plants.
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