Browse publications by year: 2017

  1. Yan, S. W.., Asmah, R.
    MyJurnal
    Practiced as folk medicine since ancient times, bilimbi (Averrhoa bilimbi) is commonly
    consumed and widely cultivated in Malaysia. In search for naturally occurring anticancer
    agents, a potential fruit extract was found to exert anticancer properties in vitro without any
    cytotoxic effect on normal cells. This study investigated the anti-proliferative effect and
    underlying cell death pathway induced by bilimbi ethanolic extract on human non-hormonedependent
    breast cancer cell line, MDA-MB-231. Anticancer potential of bilimbi extract was
    conducted by investigating the in vitro growth inhibitory effect, DNA fragmentation, cell cycle
    progression and anti-proliferation assay. Release of caspases, cytochrome c and apoptotic
    proteins were demonstrated to determine the mechanism of cell death pathway. Findings
    revealed that bilimbi inhibited growth of MDA-MB-231 cells through the induction of
    apoptosis mediated by cell cycle arrest at G0/G1 checkpoint. Released of cytochrome c coupled
    with up-regulation of caspase-3/7, caspase-9 and Bax pro-apoptotic proteins in addition to
    down-regulation of dysfunctional p53 and Bcl-2 anti-apoptotic proteins implied that bilimbi
    induced a p53-independent mitochondrial apoptosis pathway in MDA-MB-231. These results
    suggest that bilimbi could induce tumor cell anti-proliferation through apoptosis. As a natural
    product, bilimbi could be a promising anticancer agent and an inexpensive approach to cancer
    chemoprevention strategy.
  2. Mohd Khairul Hazwan Yusof, Azizah Hanom Ahmad
    Science Letter, 2017;11(1):18-23.
    MyJurnal
    Sodium solid electrolyte based on sodium dicyanamide, NaN(CN)2 doped with sodium iodide, NaI was prepared by ultra-fined grinding using planetary milling and pelletised with pellet press. Electrical conductivity and dielectric permittivity study were carried out by Electrochemical impedance spectroscopy (EIS) at frequency range between 50 Hz to 5.00 x 106 Hz. Highest conducting sample at room temperature condition managed to achieved by optimizing the doped sodium salt content with weight composition of 10NaI–90NaN(CN)2 with ionic conductivity value of (5.67 ± 1.26) x 10-4 S cm-1 at ambient temperature condition. The findings are further supported with 10 wt.% NaI in solid electrolyte system sample possessed highest dielectric constant and dielectric loss values at lower frequency region that showed non-Debye behavior for charge storage. Transference number measurement were performed and obtained a value of 0.96 that confirmed the sodium solid electrolyte sample is ionic conductor in nature.
  3. Nurmalessa Muhammad, Nor Zaini Zakaria, Sulaiman Shaari, Ahmad Maliki Omar
    Science Letter, 2017;11(1):10-17.
    MyJurnal
    This paper presents the findings of a study conducted to evaluate the performance and to identify faults of a 10-year old 1.1kW grid-connected photovoltaic (GCPV) system installed at Green Energy Research Center (GERC), Universiti Teknologi MARA (UiTM), Shah Alam. The system performance was evaluated by comparing the real operating field data with the data declared by the manufacturer. Eight performance indicators were used, which are: reference yield, array yield, final yield, capture losses, system losses, performance ratio, PV array efficiency and total efficiency. The fault detection has used three techniques - which were visual inspection as outlined in the International Energy Agency (IEA) document, thermal imager technique and the I-V curve characteristics. The finding for the system performance evaluation gives a 13.2% difference between the real operating field data and the declared manufacturer data. Among the faults detected from the visual inspection are browning and cracks of PV modules.
  4. Mohd Hamizan Selamat, Azizah Hanom Ahmad
    Science Letter, 2017;11(1):7-9.
    MyJurnal
    Application of dye-sensitized photoelectrochemical cell (DSSC) was developed using bio-based hybrid polyurethane (PU) polymer and composite electrolyte of Sodium iodide as cation of charge transport. The conducting electron transport material for the regenerative mechanism for Redox couple (I-1/I-3) was due corrosion in contacts of electrode. The polymer as additive has leveraged conductivity level of PU-composite electrolyte prepared by varying amount of Sodium iodide (NaI) via solution casting technique. These properties of composite electrolyte exhibited photoelectrochemical cell that was least corrosive (Block Membrane) for bio-based polymer electrolyte. DSSC design of heterojunction cell requires essential need of functions such as light absorption, charge regeneration -separation and transport to electrodes for a complete cell to work. The addition of polymer composite electrolyte in the redox energy separation of electrical transport was effective for bulk material of the DSSC cell system. The electrical conductivity of electrolyte material was evaluated as fair (x10−5 S.cm-1) using electrical impedance (EIS) with efficiency performance of photo-electro conversion. The hybrid-dye-sensitized solar cell of PU-Cu/TiO2-dye/PU-NaI-I2/B-AC configuration gave a response under light intensity of 100 mW cm−2 with 3.9% conversion efficiency with current density, Jsc of 0.06 mA cm−2 and open circuit voltage, Voc of 0.14 V respectively.
  5. Noor Farhana Yusoff, Nor Zaini Zakaria, Hedzlin Zainuddin, Sulaiman Shaari
    Science Letter, 2017;11(1):1-6.
    MyJurnal
    This paper presents the findings of a study to determine the effects of immediate built environment (mounting configuration) on output generated by photovoltaic (PV) module for building integrated photovoltaic (BIPV) and retrofitted PV systems under Malaysian climate. All the systems under study are gridconnected PV (GCPV) system. Eight GCPV systems used in this study; four for BIPV systems and the other four for retrofitted systems. Data for PV module temperature, ambient temperature, plane-of-array solar irradiance and AC power were logged at 5-min interval for all systems. The operating temperature were analysed as the temperature differential with respect to the ambient temperature. The mounting factor was established for both mounting configuration type.
  6. Rajaram N, Mariapun S, Eriksson M, Tapia J, Kwan PY, Ho WK, et al.
    Breast Cancer Res Treat, 2017 01;161(2):353-362.
    PMID: 27864652 DOI: 10.1007/s10549-016-4054-y
    PURPOSE: Mammographic density is a measurable and modifiable biomarker that is strongly and independently associated with breast cancer risk. Paradoxically, although Asian women have lower risk of breast cancer, studies of minority Asian women in predominantly Caucasian populations have found that Asian women have higher percent density. In this cross-sectional study, we compared the distribution of mammographic density for a matched cohort of Asian women from Malaysia and Caucasian women from Sweden, and determined if variations in mammographic density could be attributed to population differences in breast cancer risk factors.

    METHODS: Volumetric mammographic density was compared for 1501 Malaysian and 4501 Swedish healthy women, matched on age and body mass index. We used multivariable log-linear regression to determine the risk factors associated with mammographic density and mediation analysis to identify factors that account for differences in mammographic density between the two cohorts.

    RESULTS: Compared to Caucasian women, percent density was 2.0% higher among Asian women (p 

    MeSH terms: Breast Neoplasms/etiology; Breast Neoplasms/epidemiology*; Cross-Sectional Studies; Female; Humans; Malaysia/epidemiology; Mammography; Middle Aged; Population Surveillance; Risk Factors; Sweden/epidemiology; European Continental Ancestry Group*; Asian Continental Ancestry Group*; Breast Density*
  7. Dasan YK, Bhat AH, Ahmad F
    Carbohydr Polym, 2017 Feb 10;157:1323-1332.
    PMID: 27987839 DOI: 10.1016/j.carbpol.2016.11.012
    The current research discusses the development of poly (lactic acid) (PLA) and poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) reinforced nanocrystalline cellulose bionanocomposites. The nanocrystalline cellulose was derived from waste oil palm empty fruit bunch fiber by acid hydrolysis process. The resulting nanocrystalline cellulose suspension was then surface functionalized by TEMPO-mediated oxidation and solvent exchange process. Furthermore, the PLA/PHBV/nanocrystalline cellulose bionanocomposites were produced by solvent casting method. The effect of the addition of nanocrystalline cellulose on structural, morphology, mechanical and barrier properties of bionanocomposites was investigated. The results revealed that the developed bionanocomposites showed improved mechanical properties and decrease in oxygen permeability rate. Therefore, the developed bio-based composite incorporated with an optimal composition of nanocrystalline cellulose exhibits properties as compared to the polymer blend.
    MeSH terms: Cellulose/chemistry*; Polyesters/chemistry*; Product Packaging*; Nanoparticles/chemistry*; Nanocomposites/chemistry*
  8. R NFN, Nur Hanani ZA
    Carbohydr Polym, 2017 Feb 10;157:1479-1487.
    PMID: 27987859 DOI: 10.1016/j.carbpol.2016.11.026
    This study investigated the effects of different types of plant oil (olive oil, corn oil, soybean oil and sunflower oil) on the physical and mechanical properties of kappa-carrageenan films from Euchema cottoni species. The incorporation of plant oils increased the film thickness significantly (P<0.05). However, the moisture content, solubility and tensile strength of films decreased significantly (P<0.05) as plant oils were added. The incorporation of plant oils also contributed to a plasticizing effect, whereby the values for elongation at break increased significantly (P<0.05), from 22.3% to 108.8%. Higher oil content also led to carrageenan films with lower opacity, which contradicted with previous studies. In conclusion, the plant oils used in this research significantly improved film properties, thus demonstrating the potential of these materials to be used as food packaging films and coatings.
    MeSH terms: Carrageenan/chemistry*; Plant Oils/chemistry*; Solubility; Food Packaging*; Mechanical Phenomena
  9. Chen YW, Lee HV, Abd Hamid SB
    Carbohydr Polym, 2017 Feb 10;157:1511-1524.
    PMID: 27987863 DOI: 10.1016/j.carbpol.2016.11.030
    Cellulose in nanostructures was successfully isolated from empty fruit bunch biomass via a novel one-pot oxidative-hydrolysis technique. The physicochemical properties of nanocellulose prepared via one-pot process have shown comparable characteristics as products isolated via conventional multistep purification approach (namely dewaxing, chlorite bleaching process, alkalization, and acid hydrolysis). The chemical composition study indicated that the one-pot oxidative-hydrolysis process successfully extracted cellulose (91.0%), with the remaining minority being hemicellulose and lignin (∼6%) in the final product. Crystallinity profile of one-pot treated product (80.3%) was higher than that of multistep isolated nanocellulose (75.4%), which indicated that the disorder region (amorphous) in cellulose fibers was successfully removed. In additional to that, the morphology study demonstrated that nanocellulose prepared by one-pot process rendered spider-web-like network nanostructure, with an average diameter of fibers at a range of 51.6±15.4nm. The nanocellulose product showed high thermal stability (320°C), which was ready for nanocomposite application. One-pot oxidative-hydrolysis technique is a simple and versatile route for the preparation of nanocellulose from complex biomass within 90°C and 6h period, with minimum wastewater as compared to the multistep process.
    MeSH terms: Cellulose/chemical synthesis*; Cellulose/metabolism; Fruit/chemistry*; Hydrolysis; Lignin; Oxidation-Reduction; Arecaceae/chemistry*
  10. Habiba U, Siddique TA, Joo TC, Salleh A, Ang BC, Afifi AM
    Carbohydr Polym, 2017 Feb 10;157:1568-1576.
    PMID: 27987870 DOI: 10.1016/j.carbpol.2016.11.037
    A chitosan/polyvinyl alcohol (PVA)/zeolite composite was fabricated in this study. The composite was analyzed through field emission scanning electron microscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis, and weight loss test. FTIR and XRD results revealed a strong interaction among chitosan, PVA, and zeolite. Weight loss test results indicated that the composite was stable in acidic and basic media. Congo red was removed through flocculation, and the removal rate was 94% at an initial concentration of 100mg/L for a dose of 1g/L. The removal rate of methyl orange was controlled by adsorption at an initial concentration of less than 100mg/L. Flocculation occurred at high concentrations. The removal rate was also 94% at an initial concentration of 500mg/L for a dose of 5g/L. The adsorption behavior of the composite for the removal of methyl orange and Cr(VI) was described by using a pseudo-second-order kinetic model. The adsorption capacity of the composite for Cr(VI) was 450mg/g. Therefore, the synthesized composite exhibited versatility during the removal of dyes and heavy metals.
    MeSH terms: Adsorption; Azo Compounds; Chromium; Congo Red; Coloring Agents; Flocculation; Fourier Analysis; Kinetics; Microscopy, Electron, Scanning; Polyvinyl Alcohol; X-Ray Diffraction; Weight Loss; Spectroscopy, Fourier Transform Infrared; Zeolites; Metals, Heavy; Chitosan
  11. Mohamed MA, W Salleh WN, Jaafar J, Ismail AF, Abd Mutalib M, Mohamad AB, et al.
    Carbohydr Polym, 2017 Feb 10;157:1892-1902.
    PMID: 27987909 DOI: 10.1016/j.carbpol.2016.11.078
    This research involves the rare utilisation of the kapok fibre (Ceiba pentandra) as a raw material for the fabrication of cellulose nanocrystal (CNC) and self-assembled CNC membranes. The isolation of CNC from Ceiba pentandra began with the extraction of cellulose via the chemical alkali extraction by using 5wt% NaOH, followed by the typical acidified bleaching method and, finally, the CNC production through acid hydrolysis with 60wt% H2SO4 at the optimum time of 60min. The prepared CNC was then employed for the preparation of self-assembled membrane through the water suspension casting evaporation technique. The obtained CNC membrane was characterised in terms of its composition, crystallinity, thermal stability, as well as, structural and morphological features with the use of several techniques including FTIR, XRD, AFM, TEM, FESEM, and TGA. The FESEM and AFM analyses had illustrated the achievement of a self-assembled CNC membrane with a smooth surface and a well-distributed nano-porous structure, with the porosity of 52.82±7.79%. In addition, the findings proved that the self-assembled CNC membrane displayed good adsorption capability indicated by the recorded efficiency of 79% and 85% for 10mg/L and 5mg/L of methylene blue in an aqueous solution, respectively.
    MeSH terms: Cellulose*; Hydrolysis; Membranes, Artificial*; Ceiba/chemistry*; Nanoparticles*; Nanopores
  12. Shariful MI, Sharif SB, Lee JJL, Habiba U, Ang BC, Amalina MA
    Carbohydr Polym, 2017 Feb 10;157:57-64.
    PMID: 27987964 DOI: 10.1016/j.carbpol.2016.09.063
    In this study, chitosan/poly (ethylene oxide) nanofibres were fabricated at different chitosan:PEO weight ratio by electrospinning process. The effects of chitosan/PEO composition onto adsorption capability for Cu(II), Zn(II) and Pb(II) ions were studied. Formation of beadless fibres were achieved at 60:40 chitosan:PEO ratio. Average fiber diameter, maximum tensile strength and the specific surface area of the beadless fibres were found to be 115±31nm, 1.58MPa and 218m2/g, respectively. Chitosan/PEO composition that produced beadless fibres tend to possess higher hydrophilicity and maximum specific surface area. These characteristics lead the beadless fibres to the maximum adsorption capability. Adsorption equilibrium data were analysed by Langmuir and Freundlich isotherm. Freundlich isotherm showed the better fit with the experimental data and proved the existence of the monolayer adsorption conditions. The maximum adsorption capacity of the beadless fibres for Cu(II), Zn(II) and Pb(II) ions were found to be 120, 117 and 108mgg-1, respectively.
    MeSH terms: Adsorption; Ethylene Oxide/chemistry*; Hydrogen-Ion Concentration; Kinetics; Metals, Heavy/isolation & purification*; Chitosan/analysis*; Nanofibers*
  13. Nawaz A, Wong TW
    Carbohydr Polym, 2017 Feb 10;157:906-919.
    PMID: 27988008 DOI: 10.1016/j.carbpol.2016.09.080
    This study investigated transdermal drug delivery mechanisms of chitosan nanoparticles with the synergistic action of microwave in skin modification. Chitosan nanoparticles, with free or conjugated 5-fluorouracil, were prepared by nanospray-drying technique. Their transdermal drug delivery profiles across untreated and microwave-treated skins (2450MHz 5min, 5+5min; 3985MHz 5min) were examined. Both constituent materials of nanoparticles and drug encapsulation were required to succeed transdermal drug delivery. The drug transport was mediated via nanoparticles carrying drug across the skin and/or diffusion of earlier released drug molecules from skin surfaces. The drug/nanoparticles transport was facilitated through constituent nanoparticles and microwave fluidizing protein/lipid domains of epidermis and dermis (OH, NH, CH, CN) and dermal trans-to-gauche lipid conformational changes. The microwave induced marked changes to the skin ceramide content homogeneity. The chitosan nanoparticles largely affected the palmitic acid and keratin domains. Combined microwave and nanotechnologies synergize transdermal drug delivery.
    MeSH terms: Administration, Cutaneous; Animals; Fluorouracil/administration & dosage*; Male; Microwaves*; Skin; Skin Absorption*; Drug Delivery Systems*; Rats, Sprague-Dawley; Chitosan/chemistry; Nanoparticles/chemistry*
  14. Yusuf SNF, Azzahari AD, Selvanathan V, Yahya R, Careem MA, Arof AK
    Carbohydr Polym, 2017 Feb 10;157:938-944.
    PMID: 27988011 DOI: 10.1016/j.carbpol.2016.10.032
    A binary salt system utilizing lithium iodide (LiI) as the auxiliary component has been introduced to the N-phthaloylchitosan (PhCh) based gel polymer electrolyte consisting of ethylene carbonate (EC), dimethylformamide (DMF), tetrapropylammonium iodide (TPAI), and iodine (I2) in order to improve the performance of dye-sensitized solar cell (DSSC) with efficiency of 6.36%, photocurrent density, JSC of 17.29mAcm-2, open circuit voltage, VOC of 0.59V and fill factor, FF of 0.62. This efficiency value is an improvement from the 5.00% performance obtained by the DSSC consisting of only TPAI single salt system. The presence of the LiI in addition to the TPAI improves the charge injection rates and increases the iodide contribution to the total conductivity and both factors contribute to the increase in efficiency of the DSSC. The interaction behavior between polymer-plasticizer-salt was thoroughly investigated using EIS, FTIR spectroscopy and XRD.
    MeSH terms: Quaternary Ammonium Compounds; Chitin; Dimethylformamide; Dioxolanes; Electrolytes; Iodides; Iodine; Lithium; Plasticizers; Polymers; Spectroscopy, Fourier Transform Infrared; Sodium Chloride, Dietary; Volatile Organic Compounds
  15. Siti-Azrin AH, Norsa'adah B, Naing NN
    BMC Res Notes, 2017 Dec 06;10(1):705.
    PMID: 29212521 DOI: 10.1186/s13104-017-2990-1
    BACKGROUND: Nasopharyngeal carcinoma (NPC) exhibits a distinctive racial and geographic distribution. Many studies have reported varied significant prognostic factors affect the survival of NPC patients. Hence, this current study aimed to identify the prognostic factors of NPC patients registered in a tertiary referral hospital.

    METHODS: The records of one hundred and thirty-four NPC cases confirmed by histopathology in Hospital Universiti Sains Malaysia (USM) between 1st January 1998 and 31st December 2007 that fulfilled the inclusion and exclusion criteria were retrospectively reviewed. Simple and multiple Cox proportional hazard regression analyses were performed to determine the significant prognostic factors affect the survival of NPC patients.

    RESULTS: The mean (SD) age of patients diagnosed with NPC was 48.12 (15.88) years with Malay was the largest ethnic group compared to other ethnicities. Most of patients had locally advanced stage IV (40.6%) and stage III (39.1%) of NPC. The overall median survival time of NPC patients was 31.30 months (95% CI 23.76, 38.84). The significant prognostic factors that influenced the survival of NPC patients were older age (HR 1.03, 95% CI 1.01, 1.04), metastases (HR 2.52, 95% CI 1.01, 6.28) and stage IV disease (HR 4.50, 95% CI 1.66, 9.88).

    CONCLUSION: Older age, the presence of metastases and late stage are significant prognostic factors that influence the survival of NPC. Therefore, it is important to provide education to public and to raise awareness to diagnose NPC at an earlier stage and before the presence of metastases.

    MeSH terms: Adult; Carcinoma/pathology*; Female; Humans; Malaysia; Male; Middle Aged; Nasopharyngeal Neoplasms/pathology*; Neoplasm Metastasis; Prognosis; Referral and Consultation; Retrospective Studies; Survival Rate; Tertiary Care Centers*
  16. Khan MB, Nisar H, Ng CA, Yeap KH, Lai KC
    Microsc Microanal, 2017 12;23(6):1130-1142.
    PMID: 29212566 DOI: 10.1017/S1431927617012673
    Image processing and analysis is an effective tool for monitoring and fault diagnosis of activated sludge (AS) wastewater treatment plants. The AS image comprise of flocs (microbial aggregates) and filamentous bacteria. In this paper, nine different approaches are proposed for image segmentation of phase-contrast microscopic (PCM) images of AS samples. The proposed strategies are assessed for their effectiveness from the perspective of microscopic artifacts associated with PCM. The first approach uses an algorithm that is based on the idea that different color space representation of images other than red-green-blue may have better contrast. The second uses an edge detection approach. The third strategy, employs a clustering algorithm for the segmentation and the fourth applies local adaptive thresholding. The fifth technique is based on texture-based segmentation and the sixth uses watershed algorithm. The seventh adopts a split-and-merge approach. The eighth employs Kittler's thresholding. Finally, the ninth uses a top-hat and bottom-hat filtering-based technique. The approaches are assessed, and analyzed critically with reference to the artifacts of PCM. Gold approximations of ground truth images are prepared to assess the segmentations. Overall, the edge detection-based approach exhibits the best results in terms of accuracy, and the texture-based algorithm in terms of false negative ratio. The respective scenarios are explained for suitability of edge detection and texture-based algorithms.
    MeSH terms: Algorithms; Bacteria; Gold; Image Processing, Computer-Assisted; Microscopy, Phase-Contrast; Sewage; Cluster Analysis; Artifacts; Waste Water
  17. Tan JW, Gupta T, Manosroi W, Yao TM, Hopkins PN, Williams JS, et al.
    JCI Insight, 2017 12 07;2(23).
    PMID: 29212952 DOI: 10.1172/jci.insight.95992
    Compared with persons of European descent (ED), persons of African descent (AD) have lower aldosterone (ALDO) levels, with the assumption being that the increased cardiovascular disease (CVD) risk associated with AD is not related to ALDO. However, the appropriateness of the ALDO levels for the volume status in AD is unclear. We hypothesized that, even though ALDO levels are lower in AD, they are inappropriately increased, and therefore, ALDO could mediate the increased CVD in AD. To test this hypothesis, we analyzed data from HyperPATH - 1,788 individuals from the total cohort and 765 restricted to ED-to-AD in a 2:1 match and genotyped for the endothelin-1 gene (EDN1). Linear regression analyses with adjustments were performed. In the total and restricted cohorts, PRA, ALDO, and urinary potassium levels were significantly lower in AD. However, in the AD group, greater ALDO dysregulation was present as evidenced by higher ALDO/plasma renin activity (PRA) ratios (ARR) and sodium-modulated ALDO suppression-to-stimulation indices. Furthermore, EDN1 minor allele carriers had significantly greater ARRs than noncarriers but only in the AD group. ARR levels were modulated by a significant interaction between EDN1 and AD. Thus, EDN1 variants may identify particularly susceptible ADs who will be responsive to treatment targeting ALDO-dependent pathways (e.g., mineralocorticoid-receptor antagonists).
    MeSH terms: Adult; Aldosterone/metabolism*; Animals; Cells, Cultured; Female; Genotype; Humans; Male; Middle Aged; Potassium/urine; Renin/blood; Cohort Studies; Zona Glomerulosa/metabolism; Rats, Wistar; Sodium Chloride, Dietary/administration & dosage; Endothelin-1/genetics*; Endothelin-1/metabolism; African Continental Ancestry Group/genetics*; Young Adult
  18. Miard P, Nekaris KAI, Ramlee H
    Hum Ecol Interdiscip J, 2017 11 07;45(6):823-831.
    PMID: 29213178 DOI: 10.1007/s10745-017-9954-x
    Local ecological knowledge (LEK) increases understanding of certain species and the threats they face, especially little-studied taxa for which data on distribution and conservation are often lacking. We conducted 111 semi-structured interviews in Sarawak, Malaysia, to collect local knowledge about the behavior and distribution of the Philippine slow loris (Nycticebus menagensis) from two ethnic groups, the Iban and the Penan. Our study revealed that male Penan respondents, generally hunters, who frequently go into the forest were better at identifying animals from pictures. Overall, the Penan have a more detailed knowledge of slow loris behaviors, habitat, and distribution than the Iban. The two ethnic groups have different attitudes towards slow loris as the Penan hunt, eat, or keep them as pets while the Iban consider them sacred and signifiers of good luck. We advocate the use of LEK for providing complementary information to scientific methods in the study of cryptic animals.
    MeSH terms: Animals; Attitude; Ecology; Ethnic Groups; Humans; Lorisidae; Malaysia; Male; Philippines; Surveys and Questionnaires; Ecosystem; Knowledge; Forests
  19. Jaber AAS, Khan AH, Sulaiman SAS
    PMID: 29214026 DOI: 10.1186/s40545-017-0124-8
    Background: Evaluating outcomes after tuberculosis (TB) treatment can help identify the primary reasons for treatment success or failure. However, Yemen has a treatment success rate that remains below the World Health Organization's target. This study aimed to identify factors that were associated with unsuccessful treatment and prolonged treatment (>1 year).

    Method: Newly diagnosed cases of smear-positive pulmonary TB were prospectively followed at two centers (Taiz and Alhodidah, Yemen) between April 2014 and March 2015. Standardized forms were used to obtain information from the patients regarding their socio-demographic and clinical characteristics, treatment duration, and TB-related information. Multivariate logistic regression analyses were performed to identify factors that were associated with unsuccessful treatment and prolonged treatment (>1 year).

    Results: The study included data from 273 cases of newly diagnosed TB, with treatment being successful in 227 cases (83.1%) and unsuccessful in 46 cases (16.9%). Among the 46 patients with unsuccessful treatment, 29 patients (10.6%) stopped treatment, 6 patients (2.2%) transferred to another facility, 6 patients (2.2%) experienced treatment failure, and 5 patients (1.8%) died. The multivariate logistic regression analyses revealed that unsuccessful treatment was associated with female sex, illiterate status, and the presence of comorbidities. Prolonged treatment durations were associated with living in a rural area, smoking, chewing khat, a cough that lasted for >3 weeks at the beginning of treatment, and bilateral cavities during radiography.

    Conclusion: These results confirm that the treatment success rate in Yemen is lower than the World Health Organization's target for smear-positive pulmonary tuberculosis. Targeting the risk factors that we identified may help improve treatment outcomes. Furthermore, it may not be prudent to re-treat patients using first-line TB drugs after an initial treatment failure.

  20. Drewes JL, White JR, Dejea CM, Fathi P, Iyadorai T, Vadivelu J, et al.
    PMID: 29214046 DOI: 10.1038/s41522-017-0040-3
    Colorectal cancer (CRC) remains the third most common cancer worldwide, with a growing incidence among young adults. Multiple studies have presented associations between the gut microbiome and CRC, suggesting a link with cancer risk. Although CRC microbiome studies continue to profile larger patient cohorts with increasingly economical and rapid DNA sequencing platforms, few common associations with CRC have been identified, in part due to limitations in taxonomic resolution and differences in analysis methodologies. Complementing these taxonomic studies is the newly recognized phenomenon that bacterial organization into biofilm structures in the mucus layer of the gut is a consistent feature of right-sided (proximal), but not left-sided (distal) colorectal cancer. In the present study, we performed 16S rRNA gene amplicon sequencing and biofilm quantification in a new cohort of patients from Malaysia, followed by a meta-analysis of eleven additional publicly available data sets on stool and tissue-based CRC microbiota using Resphera Insight, a high-resolution analytical tool for species-level characterization. Results from the Malaysian cohort and the expanded meta-analysis confirm that CRC tissues are enriched for invasive biofilms (particularly on right-sided tumors), a symbiont with capacity for tumorigenesis (Bacteroides fragilis), and oral pathogens including Fusobacterium nucleatum, Parvimonas micra, and Peptostreptococcus stomatis. Considered in aggregate, species from the Human Oral Microbiome Database are highly enriched in CRC. Although no detected microbial feature was universally present, their substantial overlap and combined prevalence supports a role for the gut microbiota in a significant percentage (>80%) of CRC cases.
    MeSH terms: Gastrointestinal Microbiome; Adult; Bacteroides fragilis; Feces; Humans; Malaysia; Mucus; Peptostreptococcus; RNA, Ribosomal, 16S; Colorectal Neoplasms; Cohort Studies; Incidence; Prevalence; Fusobacterium nucleatum; Sequence Analysis, DNA; Biofilms; Carcinogenesis; Microbiota
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