Browse publications by year: 2017

  1. Ilias IA, Airianah OB, Baharum SN, Goh HH
    Data Brief, 2017 Dec;15:320-323.
    PMID: 29214193 DOI: 10.1016/j.dib.2017.09.050
    Expansin increases cell wall extensibility to allow cell wall loosening and cell expansion even in the absence of hydrolytic activity. Previous studies showed that excessive overexpression of expansin gene resulted in defective growth (Goh et al., 2014; Rochange et al., 2001) [1,2] and altered cell wall chemical composition (Zenoni et al., 2011) [3]. However, the molecular mechanism on how the overexpression of non-enzymatic cell wall protein expansin can result in widespread effects on plant cell wall and organ growth remains unclear. We acquired transcriptomic data on previously reported transgenic Arabidopsis line (Goh et al., 2014) [1] to investigate the effects of overexpressing a heterologus cucumber expansin gene (CsEXPA1) on the global gene expression pattern during early and late phases of etiolated hypocotyl growth.
    MeSH terms: Cell Wall; Arabidopsis; Hypocotyl; Cucumis sativus; Transcriptome; Etiolation
  2. Abdullah N, Kamarudin SK, Shyuan LK, Karim NA
    Nanoscale Res Lett, 2017 Dec 06;12(1):613.
    PMID: 29214597 DOI: 10.1186/s11671-017-2379-z
    Platinum (Pt) is the common catalyst used in a direct methanol fuel cell (DMFC). However, Pt can lead towards catalyst poisoning by carbonaceous species, thus reduces the performance of DMFC. Thus, this study focuses on the fabrication of a new composite TiO2 carbon nanofiber anodic catalyst support for direct methanol fuel cells (DMFCs) via electrospinning technique. The distance between the tip and the collector (DTC) and the flow rate were examined as influencing parameters in the electrospinning technique. To ensure that the best catalytic material is fabricated, the nanofiber underwent several characterizations and electrochemical tests, including FTIR, XRD, FESEM, TEM, and cyclic voltammetry. The results show that D18, fabricated with a flow rate of 0.1 mLhr-1 and DTC of 18 cm, is an ultrafine nanofiber with the smallest average diameter, 136.73 ± 39.56 nm. It presented the highest catalyst activity and electrochemical active surface area value as 274.72 mAmg-1 and 226.75m2 g-1PtRu, respectively, compared with the other samples.
  3. Mohd-Padil H, Damiri N, Sulaiman S, Chai SF, Nathan S, Firdaus-Raih M
    Sci Rep, 2017 12 07;7(1):17173.
    PMID: 29215024 DOI: 10.1038/s41598-017-17356-4
    The Burkholderia genus includes many species that are known to survive in diverse environmental conditions including low nutrient environments. One species, Burkholderia pseudomallei is a versatile pathogen that can survive in a wide range of hosts and environmental conditions. In this study, we investigated how a nutrient depleted growth environment evokes sRNA mediated responses by B. pseudomallei. Computationally predicted B. pseudomallei D286 sRNAs were mapped to RNA-sequencing data for cultures grown under two conditions: (1) BHIB as a nutrient rich media reference environment and (2) M9 media as a nutrient depleted stress environment. The sRNAs were further selected to identify potentially cis-encoded systems by investigating their possible interactions with their flanking genes. The mappings of predicted sRNA genes and interactions analysis to their flanking genes identified 12 sRNA candidates that may possibly have cis-acting regulatory roles that are associated to a nutrient depleted growth environment. Our approach can be used for identifying novel sRNA genes and their possible role as cis-mediated regulatory systems.
    MeSH terms: Base Sequence; Biological Phenomena; RNA; Burkholderia pseudomallei; Sequence Analysis, RNA; Burkholderia
  4. AlDala'een NFD, Mohamad WNKW, Alias N, Ali AM, Shaikh Mohammed J
    Soft Matter, 2017 Dec 20;14(1):124-131.
    PMID: 29215674 DOI: 10.1039/c7sm01682d
    There is an increasing interest in bioinspired dynamic materials. Abundant illustrations of protein domains exist in nature, with remarkable ligand binding characteristics and structures that undergo conformational changes. For example, calmodulin (CaM) can have three conformational states, which are the unstructured Apo-state, Ca2+-bound ligand-exposed binding state, and compact ligand-bound state. CaM's mechanical response to biological cues is highly suitable for engineering dynamic materials. The distance between CaM globular terminals in the Ca2+-bound state is 5 nm and in the ligand-bound state is 1.5 nm. CaM's nanoscale conformational changes have been used to develop dynamic hydrogel microspheres that undergo reversible volume changes. The current work presents the fabrication and preliminary results of layer-by-layer (LbL) self-assembled Dynamic MicroCapsules (DynaMicCaps) whose multilayered shell walls are composed of polyelectrolytes and CaM. Quasi-dynamic perfusion results show that the DynaMicCaps undergo drastic volume changes, with up to ∼1500% increase, when exposed to a biochemical ligand trifluoperazine (TFP) at pH 6.3. Under similar test conditions, microcapsules without CaM also underwent volume changes, with only up to ∼290% increase, indicating that CaM's bio-responsiveness was retained within the shell walls of the DynaMicCaps. Furthermore, DynaMicCaps exposed to 0.1 M NaOH underwent volume changes, with only up to ∼580% volume increase. Therefore, DynaMicCaps represent a new class of polyelectrolyte multilayer (PEM) capsules that can potentially be used to release their payload at near physiological pH. With over 200 proteins that undergo marked, well-characterized conformational changes in response to specific biochemical triggers, several other versions of DynaMicCaps can potentially be developed.
  5. Zomorodian M, Lai SH, Homayounfar M, Ibrahim S, Pender G
    PLoS One, 2017;12(12):e0188489.
    PMID: 29216200 DOI: 10.1371/journal.pone.0188489
    Conflicts over water resources can be highly dynamic and complex due to the various factors which can affect such systems, including economic, engineering, social, hydrologic, environmental and even political, as well as the inherent uncertainty involved in many of these factors. Furthermore, the conflicting behavior, preferences and goals of stakeholders can often make such conflicts even more challenging. While many game models, both cooperative and non-cooperative, have been suggested to deal with problems over utilizing and sharing water resources, most of these are based on a static viewpoint of demand points during optimization procedures. Moreover, such models are usually developed for a single reservoir system, and so are not really suitable for application to an integrated decision support system involving more than one reservoir. This paper outlines a coupled simulation-optimization modeling method based on a combination of system dynamics (SD) and game theory (GT). The method harnesses SD to capture the dynamic behavior of the water system, utilizing feedback loops between the system components in the course of the simulation. In addition, it uses GT concepts, including pure-strategy and mixed-strategy games as well as the Nash Bargaining Solution (NBS) method, to find the optimum allocation decisions over available water in the system. To test the capability of the proposed method to resolve multi-reservoir and multi-objective conflicts, two different deterministic simulation-optimization models with increasing levels of complexity were developed for the Langat River basin in Malaysia. The later is a strategic water catchment that has a range of different stakeholders and managerial bodies, which are however willing to cooperate in order to avoid unmet demand. In our first model, all water users play a dynamic pure-strategy game. The second model then adds in dynamic behaviors to reservoirs to factor in inflow uncertainty and adjust the strategies for the reservoirs using the mixed-strategy game and Markov chain methods. The two models were then evaluated against three performance indices: Reliability, Resilience and Vulnerability (R-R-V). The results showed that, while both models were well capable of dealing with conflict resolution over water resources in the Langat River basin, the second model achieved a substantially improved performance through its ability to deal with dynamicity, complexity and uncertainty in the river system.
    MeSH terms: Cooperative Behavior; Game Theory*; Models, Theoretical*; Water Supply*
  6. Yong HS, Song SL, Lim PE, Eamsobhana P
    PLoS One, 2017;12(12):e0189325.
    PMID: 29216281 DOI: 10.1371/journal.pone.0189325
    The tephritid fruit fly Zeugodacus tau (Walker) is a polyphagous fruit pest of economic importance in Asia. Studies based on genetic markers indicate that it forms a species complex. We report here (1) the complete mitogenome of Z. tau from Malaysia and comparison with that of China as well as the mitogenome of other congeners, and (2) the relationship of Z. tau taxa from different geographical regions based on sequences of cytochrome c oxidase subunit I gene. The complete mitogenome of Z. tau had a total length of 15631 bp for the Malaysian specimen (ZT3) and 15835 bp for the China specimen (ZT1), with similar gene order comprising 37 genes (13 protein-coding genes-PCGs, 2 rRNA genes, and 22 tRNA genes) and a non-coding A + T-rich control region (D-loop). Based on 13 PCGs and 15 mt-genes, Z. tau NC_027290 (China) and Z. tau ZT1 (China) formed a sister group in the lineage containing also Z. tau ZT3 (Malaysia). Phylogenetic analysis based on partial sequences of cox1 gene indicates that the taxa from China, Japan, Laos, Malaysia, Bangladesh, India, Sri Lanka, and Z. tau sp. A from Thailand belong to Z. tau sensu stricto. A complete cox1 gene (or 13 PCGs or 15 mt-genes) instead of partial sequence is more appropriate for determining phylogenetic relationship.
    MeSH terms: Animals; Electron Transport Complex IV/genetics*; Haplotypes; Phylogeny; Species Specificity; Tephritidae/classification; Tephritidae/genetics*; Genome, Mitochondrial*
  7. Sabullah, M. K., Gansau, A. J., Ahmad, S. A., Shamaan, N. A., Khalid, A., Sulaiman, M. R.
    MyJurnal
    The environmental toxicants such as copper are known to affect vital organ especially liver.
    This study examined the effects of copper sulfate (CuSO4) on the liver morphological structure
    of P. javanicus. The untreated control, 0.1 and 0.3 mg/L CuSO4 treated groups displayed normal
    polygonal structure of the hepatocyte. However, at the concentrations of 0.5, 1.0 and 5.0 mg/L
    CuSO4, the hepatostructure was significantly affected, as shown by the increasing number of
    dilation and congestion of sinusoids, vacuolation, macrophage activities and peliosis. The
    damage level and HSI value were increased while the number of hepatic nuclei per mm2 was
    decreased with the increasing of copper concentration. In conclusion, this study shows that the
    degree of liver damage in P.javanicus is dependent to the dose exposure.
  8. Asmaa, A. A., Tajul, A.Y
    MyJurnal
    This study was performed to examine the effect of deep frying and Superheated steam (SHS)
    oven on the proximate composition, fatty acid composition, and amino acid composition of
    chicken sausage. The results showed that the moisture content statistically decreased after
    cooking. The protein content and total ash increased after cooking. The fat content showed
    insignificant reduction during SHS cooking, whereas the frying process showed significant
    increase in total fat content. The fatty acid composition was affected more by the frying process,
    which showed an increase in the proportions of SFA, PUFA, and the ratio of n-6/n-3 while the
    SHS cooking insignificantly affected the fatty acid composition. The amount of total amino
    acid showed significant increase after cooking. The present study provides possible application
    of SHS oven as a healthy technique for cooking food compared with the deep frying process.
  9. Mohamad FS, Mat Zaid MH, Abdullah J, Zawawi RM, Lim HN, Sulaiman Y, et al.
    Sensors (Basel), 2017 Dec 02;17(12).
    PMID: 29207463 DOI: 10.3390/s17122789
    This article describes chemically modified polyaniline and graphene (PANI/GP) composite nanofibers prepared by self-assembly process using oxidative polymerization of aniline monomer and graphene in the presence of a solution containing poly(methyl vinyl ether-alt-maleic acid) (PMVEA). Characterization of the composite nanofibers was carried out by Fourier transform infrared (FTIR) and Raman spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). SEM images revealed the size of the PANI nanofibers ranged from 90 to 360 nm in diameter and was greatly influenced by the proportion of PMVEA and graphene. The composite nanofibers with an immobilized DNA probe were used for the detection of Mycobacterium tuberculosis by using an electrochemical technique. A photochemical indicator, methylene blue (MB) was used to monitor the hybridization of target DNA by using differential pulse voltammetry (DPV) method. The detection range of DNA biosensor was obtained from of 10-6-10-9 M with the detection limit of 7.853 × 10-7 M under optimum conditions. The results show that the composite nanofibers have a great potential in a range of applications for DNA sensors.
    MeSH terms: Aniline Compounds; DNA; Graphite; Mycobacterium tuberculosis; Biosensing Techniques; Nanofibers*
  10. Auwal SM, Zarei M, Tan CP, Basri M, Saari N
    Nanomaterials (Basel), 2017 Dec 02;7(12).
    PMID: 29207480 DOI: 10.3390/nano7120421
    Recent biotechnological advances in the food industry have led to the enzymatic production of angiotensin I-converting enzyme (ACE)-inhibitory biopeptides with a strong blood pressure lowering effect from different food proteins. However, the safe oral administration of biopeptides is impeded by their enzymatic degradation due to gastrointestinal digestion. Consequently, nanoparticle (NP)-based delivery systems are used to overcome these gastrointestinal barriers to maintain the improved bioavailability and efficacy of the encapsulated biopeptides. In the present study, the ACE-inhibitory biopeptides were generated from stone fish (Actinopyga lecanora) protein using bromelain and stabilized by their encapsulation in chitosan (chit) nanoparticles (NPs). The nanoparticles were characterized for in vitro physicochemical properties and their antihypertensive effect was then evaluated on spontaneously hypertensive rats (SHRs). The results of a physicochemical characterization showed a small particle size of 162.70 nm, a polydispersity index (pdi) value of 0.28, a zeta potential of 48.78 mV, a high encapsulation efficiency of 75.36%, a high melting temperature of 146.78 °C and an in vitro sustained release of the biopeptides. The results of the in vivo efficacy indicated a dose-dependent blood pressure lowering effect of the biopeptide-loaded nanoparticles that was significantly higher (p < 0.05) compared with the un-encapsulated biopeptides. Moreover, the results of a morphological examination using transmission electron microscopy (TEM) demonstrated the nanoparticles as homogenous and spherical. Thus, the ACE-inhibitory biopeptides stabilized by chitosan nanoparticles can effectively reduce blood pressure for an extended period of time in hypertensive individuals.
    MeSH terms: Administration, Oral; Animals; Antihypertensive Agents; Biological Availability; Blood Pressure; Blood Pressure Determination; Bromelains; Delayed-Action Preparations; Peptidyl-Dipeptidase A; Particle Size; Rats, Inbred SHR; Temperature; Microscopy, Electron, Transmission; Chitosan; Rats; Nanoparticles
  11. Sharifi-Rad M, Varoni EM, Salehi B, Sharifi-Rad J, Matthews KR, Ayatollahi SA, et al.
    Molecules, 2017 Dec 04;22(12).
    PMID: 29207520 DOI: 10.3390/molecules22122145
    Plants of the genus Zingiber (Family Zingiberaceae) are widely used throughout the world as food and medicinal plants. They represent very popular herbal remedies in various traditional healing systems; in particular, rhizome of Zingiber spp. plants has a long history of ethnobotanical uses because of a plethora of curative properties. Antimicrobial activity of rhizome essential oil has been extensively confirmed in vitro and attributed to its chemical components, mainly consisting of monoterpene and sesquiterpene hydrocarbons such as α-zingiberene, ar-curcumene, β-bisabolene and β-sesquiphellandrene. In addition, gingerols have been identified as the major active components in the fresh rhizome, whereas shogaols, dehydrated gingerol derivatives, are the predominant pungent constituents in dried rhizome. Zingiber spp. may thus represent a promising and innovative source of natural alternatives to chemical food preservatives. This approach would meet the increasing concern of consumers aware of the potential health risks associated with the conventional antimicrobial agents in food. This narrative review aims at providing a literature overview on Zingiber spp. plants, their cultivation, traditional uses, phytochemical constituents and biological activities.
    MeSH terms: Food Preservatives/chemistry; Oils, Volatile/chemistry; Zingiberaceae/chemistry*; Phytochemicals/chemistry*
  12. Jasim HH, Sulaiman SABS, Khan AH, S Rajah UA
    J Clin Diagn Res, 2017 Sep;11(9):IC07-IC11.
    PMID: 29207737 DOI: 10.7860/JCDR/2017/25364.10630
    Introduction: Caesarean section (CS) rate has increased considerably during the past years, accounting for 15% to 25% of births. During post-CS period, moderate to severe postoperative pain is a regularly reported problem. Ideally, the intensity of postoperative pain should be predicted so as to customize analgesia.

    Aim: To document the CS rate, assess the pain intensity and preoperative factors that may predict post caesarean pain among women in the Obstetric unit of a Hospital Pulau Pinang in Malaysia.

    Materials and Methods: A retrospective chart review of 400 caesarean deliveries was conducted between January 2013 and June 2014. The study encompassed patient's demographic data and obstetrics data. The overall pain scores since the time of surgery (2, 4, 8, 12, 24 and 48 hours postoperatively at rest and while moving) were assessed by visual analogue scale (VAS). The data were analyzed by using SPSS software (version 21.0 for Windows).

    Results: The results demonstrate that within a 48 hours postoperative period, the average pain at rest and while moving was 0.40±0.013 and 0.83±0.017 (VAS score), respectively. Logistic regression identified that a higher BMI (≥30) (OR 1.056; 95% CI=1.003 to 1.113, p=0.04), an increase in operation time (> 60 minutes) (OR 1.009; 95% CI=1.000 to 1.018, p=0.049), Single women (OR 11.597; 95% CI=1.382 to 97.320, p=0.024), blood group type O (OR 1.857; 95% CI=0.543 to 2.040, p = 0.001) and general anesthesia (OR 3.689; 95% CI=1.653 to 8.232, p=0.001) were found to be independent predictors for postcaesarean pain intensity.

    Conclusion: This study concluded that CS rate is 28% among women in the obstetric unit of a Hospital Pulau Pinang and the pain experienced by the study participants was mild. Moreover, the predictive factors for pain intensity may aid in identifying patients at greater risk for postoperative pain. This study concluded that the predictive methods proposed may aid in identifying patients at greater risk for postoperative pain.

  13. Mehrtash H, Duncan K, Parascandola M, David A, Gritz ER, Gupta PC, et al.
    Lancet Oncol, 2017 12;18(12):e767-e775.
    PMID: 29208442 DOI: 10.1016/S1470-2045(17)30460-6
    Betel quid and areca nut are known risk factors for many oral and oesophageal cancers, and their use is highly prevalent in the Asia-Pacific region. Additionally, betel quid and areca nut are associated with health effects on the cardiovascular, nervous, gastrointestinal, metabolic, respiratory, and reproductive systems. Unlike tobacco, for which the WHO Framework Convention on Tobacco Control provides evidence-based policies for reducing tobacco use, no global policy exists for the control of betel quid and areca nut use. Multidisciplinary research is needed to address this neglected global public health emergency and to mobilise efforts to control betel quid and areca nut use. In addition, future research is needed to advance our understanding of the basic biology, mechanisms, and epidemiology of betel quid and areca nut use, to advance possible prevention and cessation programmes for betel quid and areca nut users, and to design evidence-based screening and early diagnosis programmes to address the growing burden of cancers that are associated with use.
    MeSH terms: Areca/adverse effects*; Asia/epidemiology; Esophageal Neoplasms/etiology; Esophageal Neoplasms/prevention & control*; Female; Humans; Male; Mouth Neoplasms/etiology; Mouth Neoplasms/prevention & control*; Policy Making; Research Design/standards; Smoking/adverse effects; Global Health; Practice Guidelines as Topic*; Early Detection of Cancer/standards*
  14. Salar U, Khan KM, Chigurupati S, Taha M, Wadood A, Vijayabalan S, et al.
    Sci Rep, 2017 12 05;7(1):16980.
    PMID: 29209017 DOI: 10.1038/s41598-017-17261-w
    Current research is based on the identification of novel inhibitors of α-amylase enzyme. For that purpose, new hybrid molecules of hydrazinyl thiazole substituted chromones 5-27 were synthesized by multi-step reaction and fully characterized by various spectroscopic techniques such as EI-MS, HREI-MS, 1H-NMR and 13C-NMR. Stereochemistry of the iminic bond was confirmed by NOESY analysis of a representative molecule. All compounds 5-27 along with their intervening intermediates 1-4, were screened for in vitro α-amylase inhibitory, DPPH and ABTS radical scavenging activities. All compounds showed good inhibition potential in the range of IC50 = 2.186-3.405 µM as compared to standard acarbose having IC50 value of 1.9 ± 0.07 µM. It is worth mentioning that compounds were also demonstrated good DPPH (IC50 = 0.09-2.233 µM) and ABTS (IC50 = 0.584-3.738 µM) radical scavenging activities as compared to standard ascorbic acid having IC50 = 0.33 ± 0.18 µM for DPPH and IC50 = 0.53 ± 0.3 µM for ABTS radical scavenging activities. In addition to that cytotoxicity of the compounds were checked on NIH-3T3 mouse fibroblast cell line and found to be non-toxic. In silico studies were performed to rationalize the binding mode of compounds (ligands) with the active site of α-amylase enzyme.
    MeSH terms: alpha-Amylases/antagonists & inhibitors*; Animals; Biphenyl Compounds; Chromones/pharmacology*; Chromones/chemistry; Computer Simulation; Drug Evaluation, Preclinical/methods; Enzyme Inhibitors/pharmacology*; Enzyme Inhibitors/chemistry; Picrates; Mass Spectrometry; Structure-Activity Relationship; Thiazoles/chemistry; Molecular Structure; Free Radical Scavengers/pharmacology*; Free Radical Scavengers/chemistry; NIH 3T3 Cells; Mice; Molecular Docking Simulation
  15. Lee WS, Ong SY, Foo HW, Wong SY, Kong CX, Seah RB, et al.
    World J Gastroenterol, 2017 Nov 21;23(43):7776-7784.
    PMID: 29209118 DOI: 10.3748/wjg.v23.i43.7776
    AIM: To examine the medical status of children with biliary atresia (BA) surviving with native livers.

    METHODS: In this cross-sectional review, data collected included complications of chronic liver disease (CLD) (cholangitis in the preceding 12 mo, portal hypertension, variceal bleeding, fractures, hepatopulmonary syndrome, portopulmonary hypertension) and laboratory indices (white cell and platelet counts, total bilirubin, albumin, international normalized ratio, alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transpeptidase). Ideal medical outcome was defined as absence of clinical evidence of CLD or abnormal laboratory indices.

    RESULTS: Fifty-two children [females = 32, 62%; median age 7.4 years, n = 35 (67%) older than 5 years] with BA (median age at surgery 60 d, range of 30 to 148 d) survived with native liver. Common complications of CLD noted were portal hypertension (40%, n = 21; 2 younger than 5 years), cholangitis (36%) and bleeding varices (25%, n = 13; 1 younger than 5 years). Fifteen (29%) had no clinical complications of CLD and three (6%) had normal laboratory indices. Ideal medical outcome was only seen in 1 patient (2%).

    CONCLUSION: Clinical or laboratory evidence of CLD are present in 98% of children with BA living with native livers after hepatoportoenterostomy. Portal hypertension and variceal bleeding may be seen in children younger than 5 years of age, underscoring the importance of medical surveillance for complications of BA starting at a young age.

    MeSH terms: Adolescent; Biliary Atresia/blood; Biliary Atresia/complications*; Biliary Atresia/surgery; Child; Child, Preschool; Cholangitis/etiology; Cholangitis/epidemiology*; Chronic Disease; Cross-Sectional Studies; Esophageal and Gastric Varices/blood; Esophageal and Gastric Varices/etiology; Esophageal and Gastric Varices/epidemiology*; Female; Follow-Up Studies; Gastrointestinal Hemorrhage/blood; Gastrointestinal Hemorrhage/etiology; Gastrointestinal Hemorrhage/epidemiology*; Humans; Hypertension, Portal/blood; Hypertension, Portal/etiology; Hypertension, Portal/epidemiology*; Liver/physiopathology; Liver/surgery; Liver Function Tests; Malaysia/epidemiology; Male; Portoenterostomy, Hepatic; Hepatopulmonary Syndrome/blood; Hepatopulmonary Syndrome/etiology; Hepatopulmonary Syndrome/epidemiology*; Fractures, Bone/blood; Fractures, Bone/etiology; Fractures, Bone/epidemiology*
  16. Amin HU, Mumtaz W, Subhani AR, Saad MNM, Malik AS
    Front Comput Neurosci, 2017;11:103.
    PMID: 29209190 DOI: 10.3389/fncom.2017.00103
    Feature extraction is an important step in the process of electroencephalogram (EEG) signal classification. The authors propose a "pattern recognition" approach that discriminates EEG signals recorded during different cognitive conditions. Wavelet based feature extraction such as, multi-resolution decompositions into detailed and approximate coefficients as well as relative wavelet energy were computed. Extracted relative wavelet energy features were normalized to zero mean and unit variance and then optimized using Fisher's discriminant ratio (FDR) and principal component analysis (PCA). A high density EEG dataset validated the proposed method (128-channels) by identifying two classifications: (1) EEG signals recorded during complex cognitive tasks using Raven's Advance Progressive Metric (RAPM) test; (2) EEG signals recorded during a baseline task (eyes open). Classifiers such as, K-nearest neighbors (KNN), Support Vector Machine (SVM), Multi-layer Perceptron (MLP), and Naïve Bayes (NB) were then employed. Outcomes yielded 99.11% accuracy via SVM classifier for coefficient approximations (A5) of low frequencies ranging from 0 to 3.90 Hz. Accuracy rates for detailed coefficients were 98.57 and 98.39% for SVM and KNN, respectively; and for detailed coefficients (D5) deriving from the sub-band range (3.90-7.81 Hz). Accuracy rates for MLP and NB classifiers were comparable at 97.11-89.63% and 91.60-81.07% for A5 and D5 coefficients, respectively. In addition, the proposed approach was also applied on public dataset for classification of two cognitive tasks and achieved comparable classification results, i.e., 93.33% accuracy with KNN. The proposed scheme yielded significantly higher classification performances using machine learning classifiers compared to extant quantitative feature extraction. These results suggest the proposed feature extraction method reliably classifies EEG signals recorded during cognitive tasks with a higher degree of accuracy.
  17. Othman M, Ariff AB, Rios-Solis L, Halim M
    Front Microbiol, 2017;8:2285.
    PMID: 29209295 DOI: 10.3389/fmicb.2017.02285
    Lactic acid bacteria are industrially important microorganisms recognized for their fermentative ability mostly in their probiotic benefits as well as lactic acid production for various applications. Nevertheless, lactic acid fermentation often suffers end-product inhibition which decreases the cell growth rate. The inhibition of lactic acid is due to the solubility of the undissociated lactic acid within the cytoplasmic membrane and insolubility of dissociated lactate, which causes acidification of cytoplasm and failure of proton motive forces. This phenomenon influences the transmembrane pH gradient and decreases the amount of energy available for cell growth. In general, the restriction imposed by lactic acid on its fermentation can be avoided by extractive fermentation techniques, which can also be exploited for product recovery.
  18. Abdul Wahab NA, Zakaria MN, Abdul Rahman AH, Sidek D, Wahab S
    Psychiatry Investig, 2017 Nov;14(6):786-794.
    PMID: 29209382 DOI: 10.4306/pi.2017.14.6.786
    Objective: The present, case-control, study investigates binaural hearing performance in schizophrenia patients towards sentences presented in quiet and noise.

    Methods: Participants were twenty-one healthy controls and sixteen schizophrenia patients with normal peripheral auditory functions. The binaural hearing was examined in four listening conditions by using the Malay version of hearing in noise test. The syntactically and semantically correct sentences were presented via headphones to the randomly selected subjects. In each condition, the adaptively obtained reception thresholds for speech (RTS) were used to determine RTS noise composite and spatial release from masking.

    Results: Schizophrenia patients demonstrated significantly higher mean RTS value relative to healthy controls (p=0.018). The large effect size found in three listening conditions, i.e., in quiet (d=1.07), noise right (d=0.88) and noise composite (d=0.90) indicates statistically significant difference between the groups. However, noise front and noise left conditions show medium (d=0.61) and small (d=0.50) effect size respectively. No statistical difference between groups was noted in regards to spatial release from masking on right (p=0.305) and left (p=0.970) ear.

    Conclusion: The present findings suggest an abnormal unilateral auditory processing in central auditory pathway in schizophrenia patients. Future studies to explore the role of binaural and spatial auditory processing were recommended.

  19. Nantha YS
    Korean J Fam Med, 2017 Nov;38(6):315-321.
    PMID: 29209469 DOI: 10.4082/kjfm.2017.38.6.315
    A prescriptive model approach in decision making could help achieve better diagnostic accuracy in clinical practice through methods that are less reliant on probabilistic assessments. Various prescriptive measures aimed at regulating factors that influence heuristics and clinical reasoning could support clinical decision-making process. Clinicians could avoid time-consuming decision-making methods that require probabilistic calculations. Intuitively, they could rely on heuristics to obtain an accurate diagnosis in a given clinical setting. An extensive literature review of cognitive psychology and medical decision-making theory was performed to illustrate how heuristics could be effectively utilized in daily practice. Since physicians often rely on heuristics in realistic situations, probabilistic estimation might not be a useful tool in everyday clinical practice. Improvements in the descriptive model of decision making (heuristics) may allow for greater diagnostic accuracy.
    MeSH terms: Clinical Decision-Making; Heuristics; Decision Making; Physicians; Problem Solving; Intuition
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