Browse publications by year: 2017

  1. Gwani AS, Asari MA, Mohd Ismail ZI
    Folia Morphol (Warsz), 2017;76(4):682-688.
    PMID: 28553850 DOI: 10.5603/FM.a2017.0049
    BACKGROUND: The foot is composed of medial, lateral and transverse arches which, particularly the medial arch, provide it with the ability to function both as a flexible and rigid structure for proper locomotion. Arches of the foot, as well as their effect on lower extremity function, have been studied. However, quantitative data on the relationship between these arches still remain scanty. The purpose of this study was, therefore, to examine how the three arches of the foot intercorrelate.

    MATERIALS AND METHODS: Seventy-six participants (58 males, 18 females) were recruited to participate in the study. Bilateral weight-bearing lateral radiographs of the right foot were taken from each participant. Navicular heights (NH), medial cuneiform height (MCH), calcaneal inclination angle (CIA) and calcaneal-first metatarsal angle (C1MA) were measured to represent the medial arch. The lateral arch was represented by cuboid height (CH) and calcaneal-fifth metatarsal angle (C5MA) whereas; MCH and CH represented the transverse arch. Mean difference of variables between males and females was compared using independent t-test while the correlation between the variables was determined using Pearson correlation.

    RESULTS: All the variables were not significantly related to gender. Significant moderate to excellent linear correlations were observed between the variables. CIA showed the strongest correlation with C1MA (r = -0.90) and C5MA (r = -0.84) whereas, CH had the least correlation with other variables.

    CONCLUSIONS: The moderate to excellent correlations between the variables indicate that deformation or elevation of the medial arch may consequently result in similar movements of the lateral and transverse arches and vice versa.

    MeSH terms: Calcaneus; Female; Foot; Humans; Locomotion; Male; Metatarsal Bones; Tarsal Bones; Weight-Bearing; Lower Extremity
  2. Gan HM, Takahashi H, Hammer MP, Tan MH, Lee YP, Voss JM, et al.
    Mitochondrial DNA B Resour, 2017 Feb 06;2(1):73-75.
    PMID: 33473721 DOI: 10.1080/23802359.2017.1285206
    The complete mitochondrial genomes of four fish species of the commercially important family Latidae were sequenced using the Illumina MiSeq, thereby significantly increasing the mitogenomic resources for the family. Whole mitogenome-based phylogenetic analysis supports the monophyly of the genus Lates and more generally the family Latidae. The mitogenome sequences from this study will be useful for future assessments of the diversity within and between Lates species and studies of phylogenetic relationships within the diverse and taxonomically challenging perciform fishes.
    MeSH terms: Animals; Fishes; Perciformes; Phylogeny; Genome, Mitochondrial
  3. Gan HM, Amornsakun T, Tan MP
    Mitochondrial DNA B Resour, 2017 Mar 17;2(1):148-149.
    PMID: 33473747 DOI: 10.1080/23802359.2017.1298418
    We sequenced and assembled three whole mitogenome sequences of the commercially important snakeskin gourami Trichopodus pectoralis isolated from Malaysia (introduced), Viet Nam (native) and Thailand (native). The mitogenome length range from 16,397 to 16,420 bp. The final partitioned nucleotide alignment consists of 14,002 bp and supports the monophyly of the genus Trichopodus (95% ultrafast bootstrap support) with T. trichopterus forming a sister group with the members of T. pectoralis.
    MeSH terms: Animals; Fishes; Malaysia; Nucleotides; Perciformes; Thailand; Vietnam; Genome, Mitochondrial
  4. Okuyama H, Tingek S, Takahashi JI
    Mitochondrial DNA B Resour, 2017 Jul 31;2(2):475-476.
    PMID: 33473869 DOI: 10.1080/23802359.2017.1361344
    The complete mitochondrial genome of the cavity-nesting honeybee Apis cerana from Sabah on Borneo Island was analyzed using next-generation sequencing. The mitochondrial genome of A. cerana was a circular molecule of 15,884 bp and was similar to that of the other cavity-nesting honeybee species. The average AT content in the A. cerana mitochondrial genome was 84.4%. It was predicted to contain 13 protein-coding, 22 tRNA, and two rRNA genes, along with one A + T-rich control region.
    MeSH terms: Animals; Bees; Borneo; Malaysia; RNA, Transfer; Genes, rRNA; Genome, Mitochondrial; High-Throughput Nucleotide Sequencing; Islands
  5. Takahashi JI, Tingek S, Okuyama H
    Mitochondrial DNA B Resour, 2017 Sep 05;2(2):585-586.
    PMID: 33473910 DOI: 10.1080/23802359.2017.1372714
    The cavity-nesting honeybee Apis nuluensis inhabits only the highlands of Mount Kinabalu of Sabah, Borneo Island. The mitochondrial genome is a circular molecule of approximately 1.6 kb that includes 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one AT-rich control region. The average AT content was 84.5%. The start codons ATC, ATG, and ATT were found in one, three, and nine genes, respectively, whereas the stop codon TAA was observed in all genes. The phylogenetic relationship, inferred using 13 PCGs, was consistent with that reported in previous studies that predicted a sister taxon relationship between A. nuluensis and A. cerana.
    MeSH terms: Animals; Bees; Borneo; Malaysia; Phylogeny; RNA, Transfer; Codon, Initiator; Codon, Terminator; Genes, rRNA; Gene Order; Genome, Mitochondrial; Islands
  6. Liu J, Niu YF, Ni SB, Liu ZY, Zheng C, Shi C
    Mitochondrial DNA B Resour, 2017 Dec 12;3(1):13-14.
    PMID: 33474051 DOI: 10.1080/23802359.2017.1413317
    The Artocarpus heterophyllus, native to Western Ghats of India, Malaysia and south-eastern Asia, is a tree member of the mulberry family (Moraceae). Chloroplast genome sequences play a significant role in the development of molecular markers in plant phylogenetic and population genetic studies. In this study, we report the complete chloroplast genome sequence of A. heterophyllus for the first time. The chloroplast genome is 160,387 bp long and includes 113 genes. Its LSC, SSC and IR regions are 88,422, 18,869 and 26,548 bp long, respectively. Phylogenetic tree analysis exhibited that A. heterophyllus was clustered with other Moraceae species with high bootstrap values.
    MeSH terms: Chloroplasts; Far East; Genetics, Population; India; Malaysia; Phylogeny; Research Design; Moraceae; Artocarpus; Morus; Genome, Chloroplast
  7. Huang X, Shan L, Cheng K, Weng W
    ACS Biomater Sci Eng, 2017 Dec 11;3(12):3254-3260.
    PMID: 33445368 DOI: 10.1021/acsbiomaterials.7b00551
    The topography at the micro/nanoscale level for biomaterial surfaces has been thought to play vital roles in their interactions with cells. However, discovering the interdisciplinary mechanisms underlying how cells respond to micro-nanostructured topography features still remains a challenge. In this work, ∼37 μm 3D printing used titanium microspheres and their further hierarchical micro-nanostructured spheres through hydrothermal treatment were adopted to construct typical model surface topographies to study the preosteoblastic cell responses (adhesion, proliferation, and differentiation). We here demonstrated that not only the hierarchical micro-nanostructured surface topography but also their distribution density played critical role on cell cytocompatibility. The microstructured topography feature surface with middle-density distributed titanium microspheres showed significantly enhanced cell responses, which might be attributed to the better cellular interaction due to the cell aggregates. However, the hierarchical micro-nanostructured topography surface, regardless of the distribution density of titanium microspheres, improved the cell-surface interactions because of the enhanced initial protein adsorption, thereby reducing the cell aggregates and consequently their responses. This work, therefore, provides new insights into the fundamental understanding of cell-material interactions and will have a profound impact on further designing micro-nanostructured topography surfaces to control cell responses.
    MeSH terms: Adsorption; Biocompatible Materials; Cell Communication; Cell Differentiation; Microspheres; Titanium; Cell Proliferation; Nanostructures; Printing, Three-Dimensional
  8. Mirasol R, Thai AC, Salahuddin AA, Tan K, Deerochanawong C, Mohamed M, et al.
    J ASEAN Fed Endocr Soc, 2017;32(1):6-12.
    PMID: 33442078 DOI: 10.15605/jafes.032.01.02
    The Asia-Pacific region carries a high disease burden, with over half of the global diabetic population residing in this region. Increasing evidence shows that without targeted intervention, the progression from impaired glucose tolerance (IGT) to type 2 diabetes occurs more frequently in Asians compared with Caucasians. Furthermore, IGT is independently associated with an increased risk of cardiovascular disease, and should be managed as early as possible. Because diabetes is now a major public health issue, strategies aimed at prevention and treatment are urgently required. Lifestyle modification, including weight loss, dietary changes and increased physical activity, play a major role in controlling the disease. Significant evidence also supports the effectiveness of a combination of lifestyle modification and pharmacologic therapy, such as metformin, in delaying the onset of diabetes. Although the importance of lifestyle interventions is well recognized throughout Asia, many countries do not have formal recommendations to guide the diagnosis and management of individuals at risk of progression to diabetes. At a recent regional meeting, experts from the Asian region convened to develop consensus recommendations to guide clinicians in the management of Asian patients with pre-diabetes. These consensus recommendations provide a clear and concise approach to the management of individuals with IGT based on the available evidence and current best clinical practice.
    MeSH terms: Asia; Behavior Therapy; Cardiovascular Diseases; Diabetes Mellitus, Type 2; Humans; Hypoglycemic Agents; Life Style; Metformin; Prediabetic State; Public Health; Weight Loss; Exercise; Cost of Illness; Glucose Intolerance; Consensus; Asian Continental Ancestry Group
  9. Abdullah NRA, Akbar RZA
    J ASEAN Fed Endocr Soc, 2017;32(1):50-53.
    PMID: 33442085 DOI: 10.15605/jafes.032.01.09
    Autoimmune thyroiditis in the course of other autoimmune diseases such as systemic lupus erythematosus (SLE) is common because these disorders are attributed to the production of autoantibodies against various autoantigens. Beyond this association, autoimmune thyroiditis can occur before, during or after the development of SLE. In this report, we describe a female who presented with facial puffiness, lethargy and progressive abdominal distension. She was diagnosed with autoimmune thyroiditis followed by the diagnosis of SLE complicated by a massive ascites, a rare form of lupus peritonitis, which is sterile ascites that results from severe serositis. Her presentation was complex and posed a diagnostic challenge and dilemma to the physicians involved in her care.
    MeSH terms: Ascites; Autoantibodies; Autoantigens; Autoimmune Diseases; Female; Humans; Lupus Erythematosus, Discoid; Lupus Erythematosus, Systemic; Peritonitis; Physicians; Serositis; Thyroiditis, Autoimmune; Hashimoto Disease; Lethargy
  10. Tong CV, Hussein Z
    J ASEAN Fed Endocr Soc, 2017;32(1):54-56.
    PMID: 33442086 DOI: 10.15605/jafes.032.01.10
    For ectopic adrenocorticotropic hormone (ACTH) syndrome (EAS), when surgery is not feasible, or in cases of severe biochemical disturbances, immunosuppression or mental instability, medical therapy with agents such as etomidate is indicated. We present our experience in using etomidate for a 41-year old female with EAS secondary to a malignant mediastinal paraganglioma. We were able to demonstrate that etomidate can be used effectively to control severe hypercortisolism in a lower dose than previously described.
    MeSH terms: ACTH Syndrome, Ectopic; Adrenocorticotropic Hormone; Cushing Syndrome; Etomidate; Female; Immunosuppression; Paraganglioma; Paraganglioma, Extra-Adrenal
  11. Loh LT, Lim V
    J ASEAN Fed Endocr Soc, 2017;32(1):57-59.
    PMID: 33442087 DOI: 10.15605/jafes.032.01.11
    Thyroid function is usually normal in differentiated thyroid carcinoma. We describe a case of a female patient who had metastatic follicular thyroid carcinoma (FTC) to the spine and lungs, who was clinically euthyroid but had very low free tetraiodothyronine (fT4) and normal thyroid stimulating hormone (TSH). Free triiodothyronine (fT3) and total T3 (TT3) were normal. Levothyroxine treatment increased fT4 marginally but caused a two- to three-fold rise in fT3 and TT3 along with suppressed TSH. This is likely due to hyperconversion of T4 to T3 from elevation in D2 deiodinase activity in the tumor. This phenomenon has been reported to occur in about 20% of metastatic FTC.
    MeSH terms: Female; Humans; Iodide Peroxidase; Lung; Spine; Thyroid Function Tests; Thyroid Neoplasms; Thyrotropin; Thyroxine; Triiodothyronine; Adenocarcinoma, Follicular
  12. Hussain S, Men KK, Majid NA
    J ASEAN Fed Endocr Soc, 2017;32(2):132-138.
    PMID: 33442096 DOI: 10.15605/jafes.032.02.06
    Objectives: We aim to compare the clinical and biochemical profile of metabolic syndrome between obese children below and above 10 years attending Paediatric clinic Hospital Universiti Sains Malaysia (HUSM) from 2006 to 2015. This is to determine if age, particularly the transition to puberty, modifies the prevalence of components of metabolic syndrome in obese children.

    Methodology: The medical records of 84 obese children under 18 years of age seen at Paediatric clinic HUSM from 2006 to 2015 were reviewed. Demographic (age, gender, ethnicity), anthropometric (weight and height), clinical [body mass index (BMI), systolic blood pressure (SBP) and diastolic blood pressure (DBP)] and biochemical [serum total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), fasting plasma glucose (FPG)] parameters were recorded, analyzed and compared.

    Results: Majority of subjects in both age groups were boys, with 68.2% <10 years old. Mean age was 9.69 years (±3.36). The clinical and biochemical parameters of metabolic syndrome were similar between those <10 years old and >10 years, with the exception of BMI, waist circumference, SBP and TG level. Multivariate regression analysis showed that the parameters of metabolic syndrome significantly associated with age ≥10 years were systolic hypertension (adjusted OR 7.17, 95% CI, 1.48 to 34.8) and BMI >30 kg/m2 (adjusted OR 3.02, 95% CI, 1.16 to 7.86).

    Conclusion: There were similar clinical and biochemical parameters of metabolic syndrome in both age groups. The proportions of children with metabolic syndrome were similar regardless of age group. The overall prevalence rate of metabolic syndrome was 27.3%. In view of the alarming presence of components of metabolic syndrome even in children less than 10 years of age, efforts aimed at the prevention of childhood obesity in the community should be intensified.

    MeSH terms: Adolescent; Blood Glucose; Blood Pressure; Child; Ethnic Groups; Fasting; Hospitals; Humans; Hypertension; Cholesterol, HDL; Cholesterol, LDL; Malaysia; Male; Medical Records; Men; Puberty; Regression Analysis; Triglycerides; Body Mass Index; Prevalence; Waist Circumference; Pediatric Obesity
  13. Dharan SS, Kamaruddin NA
    J ASEAN Fed Endocr Soc, 2017;32(2):169-172.
    PMID: 33442102 DOI: 10.15605/jafes.032.02.12
    Acromegaly is a rare disease with an annual incidence of 3 to 4 cases in a million.1 Diagnosis is often delayed due to the slow progression of the disease. Persistent elevation of growth hormone (GH) in acromegaly causes a reduction in life expectancy by 10 years. Aside from multiple cardiovascular, respiratory and metabolic co-morbidities, it has also been proven to cause an increased incidence of cancer. The main treatment of acromegaly is surgical excision of the functioning pituitary adenoma. Multiple comorbidities, including obstructive sleep apnea (OSA), left ventricular hypertrophy (LVH) and soft tissue swelling, make surgery complicated, if not impossible. Medical therapy to reduce comorbidities may be indicated in certain situations. Somatostatin receptor ligands (SRL) are able to reduce, and possibly normalize, IGF-1 levels.2 Reduction of insulin-like growth factor-1 (IGF-1), the main mediator of GH, is able to resolve headache, sweating, fatigue and soft tissue swelling, and also reduce ventricular hypertrophy. This case report illustrates the successful use of the SRL octreotide LAR in treating acromegaly. It also confirms the observation from several case series that thyroid cancer is the most common malignancy in acromegaly.
    MeSH terms: Acromegaly; Fatigue; Headache; Insulin-Like Growth Factor I; Life Expectancy; Ligands; Pituitary Neoplasms; Growth Hormone; Sweating; Thyroid Neoplasms; Octreotide; Incidence; Hypertrophy, Left Ventricular; Receptors, Somatostatin; Human Growth Hormone; Sleep Apnea, Obstructive; Rare Diseases
  14. Hashem AAR, Ahmed HMA
    Eur Endod J, 2017;2(1):1-4.
    PMID: 33403327 DOI: 10.5152/eej.2017.17042
    A comprehensive knowledge and understanding of root canal anatomical variations are essential for successful root canal treatment. Mandibular molar teeth show considerable variations in their external and internal radicular morphology that require special attention from dental practitioners to provide the best clinical outcomes to the patients. This report aims to present root canal treatment of a mandibular first molar that has six separate root canals (three root canals in the mesial roots and three in the distal roots [236 M3 D3]). This report points out the importance of proper exploration for identifying additional canals in mandibular molars.
    MeSH terms: Dental Pulp Cavity; Dentists; Humans; Molar; Root Canal Therapy; Tooth Root; Professional Role
  15. Ong TK
    Eur Endod J, 2017;2(1):1-6.
    PMID: 33403352 DOI: 10.14744/eej.2017.17035
    Objective: The purpose of this article was to report the finding of the disappearance of intracanal medication as a supporting evidence of vertical root fracture (VRF) through non-surgical intervention.

    Methods: A retrospective review of the dental records of patients seen by an endodontist in a private endodontic office from September 2013 to September 2016 was conducted by the same endodontist. Cases that met the inclusion and exclusion criteria were assigned as the subjects of this study, and data were extracted from their clinical and radiographic records. Patient's demographic features, pre-operative signs and symptoms, details of rendered clinical procedures, follow-up visits, clinical and radiographic findings were recorded. Seventeen teeth for which non-surgical exploratory re-treatment was initiated were included in this study. Calcium hydroxide-based intracanal medication was placed for 2-4 weeks. Obturation of the root canals was performed if the tooth showed improvement of clinical signs and symptoms. If not, a cone-beam computed tomography (CBCT) scan was proposed to the patient to rule out VRF.

    Results: After the non-surgical re-treatment was initiated, 13 teeth showed improvement of clinical symptoms and the re-treatment was therefore completed. The remaining 4 teeth presented with unresolved clinical presentations (deep pocket, presence of sinus tract and/or tender to percussion and palpation). Four teeth showed partial disappearance of intracanal medication where VRF was confirmed using CBCT in 3 teeth and with a conventional periapical (PA) radiograph in 1 tooth.

    Conclusion: The disappearance of intracanal medication during non-surgical intervention was often associated with VRF. Thus, this feature may serve as an aid in diagnosing VRF.

    MeSH terms: Bicuspid; Calcium Hydroxide; Demography; Dental Pulp Cavity; Dental Records; Follow-Up Studies; Humans; Molar; Palpation; Percussion; Retrospective Studies; Cone-Beam Computed Tomography; Spiral Cone-Beam Computed Tomography; Endodontists
  16. Citation: Malaysian Consensus Guidelines on Antiretroviral Therapy 2017. Kuala Lumpur: Malaysian Society for HIV Medicine; 2017
    MeSH terms: Humans; Malaysia; HIV Infections; Needlestick Injuries; Guidelines as Topic; Antiretroviral Therapy, Highly Active; Post-Exposure Prophylaxis
  17. Citation: Management of Snakebite. Putrajaya: Ministry of Health, Malaysia; 2017
    MeSH terms: Animals; Humans; Malaysia; Snake Bites; Guidelines as Topic
  18. Sambasevam, Yogesvari, Wong, Siong Jiun, Farihah Hanani Ghazali, Ammar Izzati Amir Ramadan, Mohd Roslan Sulaiman, Mohd Khairi Hussain, et al.
    MyJurnal
    Introduction: Active compounds derived from plants are able to inhibit nerve conduction. Cardamonin, a naturally occurring chalcone, manifests anti-nociceptive, anti-inflammatory and anti-neuropathy properties. Consequently, cardamonin may potentially inhibit nerve action potential, whereby, it affects the nerve conduction. Compound action potential is the sum of the activity which is measured from a nerve trunk. Objective: The experiment was carried out to investigate the inhibitory effect of cardamonin on compound action potentials and its possible mechanism of action on frog sciatic nerve. Methodology: LabTutor software was used to record compound action potentials in frog sciatic nerve. Sciatic nerve was isolated from the frog and soaked in Ringer’s solution. Stimulating electrodes were used to stimulate the nerve and recording electrodes were used to record compound action potentials. Compound action potential of the nerve were recorded before and after treatments [vehicle, cardamonin (0.5, 1 & 2 mg/ml) & morphine (3mg/ml)]. Participation of opioid system was investigated by pre-treating the nerve with naloxone and followed by cardamonin. All the data were recorded and analysed via LabTutor software. The data were analysed by using Two-way ANOVA followed by Bonferonni’s post hoc test with significant value at P < 0.05. Results: The outcomes showed that all the doses of cardamonin significantly reduced the peak amplitude of compound action potential in frog sciatic nerves. Besides, co-treatment of naloxone and cardamonin significantly (P < 0.001) reversed the effect of cardamonin on peak amplitude of compound action potential, suggesting the involvement of opioid receptors to inhibit nerve conduction. Conclusion: Cardamonin reduces the nerve signal conduction via activation of opioid receptors to modulate pain and contribute to the analgesic effects.
    MeSH terms: Action Potentials; Analgesics, Opioid; Analysis of Variance; Anti-Inflammatory Agents; Chalcone; Electrodes; Morphine; Naloxone; Neural Conduction; Pain; Receptors, Opioid; Sciatic Nerve; Chalcones
  19. Pek, Lim Chu, Chai, Hoon Khoo, Yoke, Kqueen Cheah
    MyJurnal
    Actinobacteria from underexplored and unusual environments have gained significant attention for their capability in producing novel bioactive molecules of diverse chemical entities. Streptomyces is the most prolific Actinobacteria in producing useful molecules. Rapid decline effectiveness of existing antibiotics in the treatment of infections are caused by the emergence of multidrug-resistant pathogens. Intensive efforts are urgently required in isolating non-Streptomyces or rare Actinobacteria and understanding of their distribution in the harsh environment for new drug discovery. In this study, pretreatment of soil samples with 1.5% phenol was used for the selective isolation of Actinobacteria from Dee Island and Greenwich Island. A high number of non-Streptomyces (69.4%) or rare Actinobacteria was significantly recovered despite the Streptomyces (30.6%), including the genera Micromonospora, Micrococcus, Kocuria, Dermacoccus, Brachybacterium, Brevibacterium, Rhodococcus, Microbacterium and Rothia. Reduced diversity and shift of distribution were observed at the elevated level of soil pH. The members of genera Streptomyces, Micromonospora and Micrococcus were found to distribute and tolerate to a relatively high pH level of soil (pH 9.4-9.5), and could potentially be alkaliphilic Actinobacteria. The phylogenetic analysis had revealed some potentially new taxa members of the genera Micromonospora, Micrococcus and Rhodococcus. Principal Component Analysis of soil samples was used to uncover the factors that underlie the diversity of culturable Actinobacteria. Water availability in soil was examined as the principal factor that shaped the diversity of the Actinobacteria, by providing a dynamic source for microbial interactions and elevated diversity of Actinobacteria.
    MeSH terms: Actinomycetales; Anti-Bacterial Agents; Brevibacterium; Micrococcaceae; Micrococcus; Micromonospora; Phylogeny; Rhodococcus; Soil; Streptomyces; Water; Phenol; Principal Component Analysis; Actinobacteria; Drug Discovery
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