Browse publications by year: 2020

  1. Cheng WT, Kantilal HK, Davamani F
    Malays J Med Sci, 2020 Jul;27(4):9-21.
    PMID: 32863742 MyJurnal DOI: 10.21315/mjms2020.27.4.2
    The Bacteroides fragilis (B. fragilis) produce biofilm for colonisation in the intestinal tract can cause a series of inflammatory reactions due to B. fragilis toxin (BFT) which can lead to chronic intestinal inflammation and tissue injury and play a crucial role leading to colorectal cancer (CRC). The enterotoxigenic B. fragilis (ETBF) forms biofilm and produce toxin and play a role in CRC, whereas the non-toxigenic B. fragilis (NTBF) does not produce toxin. The ETBF triggers the expression of cyclooxygenase (COX)-2 that releases PGE2 for inducing inflammation and control cell proliferation. From chronic intestinal inflammation to cancer development, it involves signal transducers and activators of transcription (STAT)3 activation. STAT3 activates by the interaction between epithelial cells and BFT. Thus, regulatory T-cell (Tregs) will activates and reduce interleukin (IL)-2 amount. As the level of IL-2 drops, T-helper (Th17) cells are generated leading to increase in IL-17 levels. IL-17 is implicated in early intestinal inflammation and promotes cancer cell survival and proliferation and consequently triggers IL-6 production that activate STAT3 pathway. Additionally, BFT degrades E-cadherin, hence alteration of signalling pathways can upregulate spermine oxidase leading to cell morphology and promote carcinogenesis and irreversible DNA damage. Patient with familial adenomatous polyposis (FAP) disease displays a high level of tumour load in the colon. This disease is caused by germline mutation of the adenomatous polyposis coli (APC) gene that increases bacterial adherence to the mucosa layer. Mutated-APC gene genotype with ETBF increases the chances of CRC development. Therefore, the colonisation of the ETBF in the intestinal tract depicts tumour aetiology can result in risk of hostility and effect on human health.
    MeSH terms: Bacteroides fragilis; Cell Survival; Hostility; Humans; Interleukin-2; Colorectal Neoplasms; Dinoprostone; Interleukin-6; Genes, APC; Germ-Line Mutation; Interleukin-17; Tumor Burden; T-Lymphocytes, Regulatory; Cyclooxygenase 2
  2. Nwawuba Stanley U, Mohammed Khadija A, Bukola AT, Omusi Precious I, Ayevbuomwan Davidson E
    Malays J Med Sci, 2020 Jul;27(4):22-35.
    PMID: 32863743 DOI: 10.21315/mjms2020.27.4.3
    Short tandem repeat (STR) typing continues to be the primary workhorse in forensic DNA profiling. Therefore, the present review discusses the prominent role of STR marker in criminal justice system. All over the world, deoxyribonucleic acid (DNA) profiling provides evidence that may be used to convict criminals, as an irrefutable proof of wrongful convictions, invaluable links to the actual perpetrators of crimes, and could also deter some offenders from committing more serious offences. Clearly, DNA profiling tools have also aided forensic scientists to re-evaluate old cases that were considered closed as a result of inadequate evidence. In carrying out this review, a comprehensive electronic literature search using PubMed, ScienceDirect, Google Scholar and Google Search were used, and all works meeting the subject matter were considered, including reviews, retrospective studies, observational studies and original articles. Case reports presented here, further demonstrates the crucial role of forensic DNA profiling in mitigating and providing compelling evidence for the resolution of crimes. For case report 1, there was a 100% match between the DNA recovered from the items found in the crime scene, and the suspect's DNA sample collected via buccal swab following 15 STR loci examination. Case report 2 further highlights the indispensable contribution of DNA database in solving crime. Therefore, it has become very necessary for developing countries like Nigeria to develop a national DNA database and make policies and legislatures that will further expand and enable the practice of forensic genetics, particularly DNA profiling.
    MeSH terms: Crime; Criminal Law; Developing Countries; DNA; Humans; Nigeria; Retrospective Studies; DNA Fingerprinting; Microsatellite Repeats; Databases, Nucleic Acid; PubMed; Forensic Sciences; Forensic Genetics; Criminals
  3. Zin NNINM, Mohamad MN, Roslan K, Abdul Wafi S, Abdul Moin NI, Alias A, et al.
    Malays J Med Sci, 2020 Jul;27(4):36-50.
    PMID: 32863744 MyJurnal DOI: 10.21315/mjms2020.27.4.4
    BACKGROUND: The spread of Plasmodium falciparum resistance in common antimalarial drugs, including artemisinin-based combination therapies, has necessitated the discovery of new drugs with novel mechanisms of action. In the present study, the in vitro antimalarial and toxicological activities of acetone, methanol, ethanol and aqueous extracts of Quercus infectoria (Q. infectoria) galls were investigated.

    METHODS: The extracts were assessed for the antimalarial potential using a malarial SYBR Green I fluorescence-based (MSF) assay, while the toxicity was screened by using brine shrimp lethality test (BSLT), haemolytic assay, and cytotoxicity assay against normal embryo fibroblast cell line (NIH/3T3) and normal kidney epithelial cell line (Vero).

    RESULTS: The acetone extract showed the highest antimalarial activity (50% inhibitory concentration, IC50 = 5.85 ± 1.64 μg/mL), followed by the methanol extract (IC50 = 10.31 ± 1.90 μg/mL). Meanwhile, the ethanol and aqueous extracts displayed low antimalarial activity with IC50 values of 20.00 ± 1.57 and 30.95 μg/mL ± 1.27 μg/mL, respectively. The significant antimalarial activity was demonstrated in all extracts and artemisinin (P < 0.05). All extracts were non-toxic to brine shrimps (50% lethality concentration, LC50 > 1000 ppm). Furthermore, no occurrence of haemolysis (< 5%) was observed in normal erythrocytes when treated with all extracts compared to Triton X-100 that caused 100% haemolysis (P < 0.05). The acetone and methanol extracts were non-toxic to the normal cell lines and statistically significant to artemisinin (P < 0.05).

    CONCLUSION: Taken together with satisfactory selectivity index (SI) values, the acetone and methanol extracts of Q. infectoria galls could serve as an alternative, promising and safe antimalarial agents.

  4. Tan CH, Abd Rashid R, Guan NC
    Malays J Med Sci, 2020 Jul;27(4):51-63.
    PMID: 32863745 MyJurnal DOI: 10.21315/mjms2020.27.4.5
    BACKGROUND: Amphetamine-type stimulant (ATS) use brings severe adverse effects to the mental well-being of an individual and it is an essential contributor to the global disease burden. Meanwhile, religiosity and religious coping might improve one's conduct, physical and mental well-being. Hence, this study aims to determine the prevalence of anxiety and depression in ATS user and their association with religiosity and religious coping.

    METHODS: It is a cross-sectional study conducted at the Department of Psychological Medicine, Universiti Malaya Medical Centre, Malaysia. The Hospital Anxiety and Depression Scale (HADS) was used to assess anxiety and depression among ATS users. Religiosity and religious coping were measured with Duke University Religious Index and Brief RCOPE.

    RESULTS: This study involved 215 ATS users. Almost half of the ATS users had either anxiety (n = 96; 44.6%) or depression (n = 108; 50.2%), which were associated with polysubstance use or having an existing psychiatric disorder. Subjects with higher religiosity and positive religious coping were less anxious or depressed. However, negative religious coping was significantly associated with anxiety and depression in ATS users.

    CONCLUSION: Anxiety and depression are prevalent in ATS users. Integrating religiosity and religious coping into the ATS users' treatment plan helps to improve their mental well-being.

  5. Wui NB, Ahmad Anuar MA, Abdul Wahid AM
    Malays J Med Sci, 2020 Jul;27(4):64-71.
    PMID: 32863746 MyJurnal DOI: 10.21315/mjms2020.27.4.6
    Background: The management of fractures around the knee in the elderly population can be challenging due to the complexity of the patients and the fracture characteristics. In this study, we aimed to investigate the short-term outcome of elderly patients who had fractures around the knee and who were treated with primary total knee arthroplasty. The study included patients who were at least 70 years old with poor bone quality and who presented with a fracture around the knee that would be difficult to treat with open reduction and internal fixation (ORIF) as well as patients who were at least 55 years old presenting with severe concomitant knee osteoarthritis.

    Methods: This is a cross-sectional study in which all the elderly patients who underwent early primary total knee replacement due to trauma around the knee at the Segamat Hospital between January 2015 and June 2019 were identified. Data were collected from clinical and operative notes. The clinical outcomes of these patients were evaluated by the range of motion of the knee and the Knee Society Score (KSS).

    Results: Ten patients were identified to have undergone this procedure. Six patients sustained supracondylar femur fractures, two patients had tibial plateau fractures and two patients had concurrent supracondylar femur and tibial plateau fractures. The mean follow-up duration was 22.3 ± 13.9 months, the mean knee score was 87.7 ± 10.0 and the mean functional knee score was 56 ± 41.9.

    Conclusion: In this cohort, good short-term outcomes close to pre-fracture condition was noted in patients who did not suffer from any complications during the post-operative period. Two patients who had surgical site infection had lower functional knee scores. Another two patients with lower knee scores experienced surgical site infection of the distal tibia and contralateral fixed flexion deformity of the knee. Early primary total knee replacement remains a viable option in treating fractures around the knee in the elderly. Infection, which in this study affected 20% of the patients, is the main deterring factor in performing this procedure.

    MeSH terms: Aged; Cross-Sectional Studies; Femur; Follow-Up Studies; Hospitals; Humans; Knee Joint; Middle Aged; Surgical Wound Infection; Tibia; Tibial Fractures; Cohort Studies; Range of Motion, Articular; Arthroplasty, Replacement, Knee; Osteoarthritis, Knee; Open Fracture Reduction
  6. Wong HJ, Harith S, Lua PL, Ibrahim KA
    Malays J Med Sci, 2020 Jul;27(4):72-84.
    PMID: 32863747 MyJurnal DOI: 10.21315/mjms2020.27.4.7
    Background: The present study examined the prevalence and predictors of malnutrition risk among post-stroke patients.

    Methods: Post-stroke patients who attended the outpatient clinics in three hospitals of Peninsular Malaysia were enrolled in the study. The risk of malnutrition was assessed using the Malnutrition Risk Screening Tool-Hospital. Data including demographic characteristics, clinical profiles, dietary nutrients intake, body mass index (BMI) and hand grip strength were collected during the survey. The crude odds ratio (OR) and adjusted odds ratio (AOR) were reported for univariate and multivariate logistic regression analyses, respectively.

    Results: Among 398 patients included in the study, 40% were classified as high-risk for malnutrition. In the multivariable logistic regression, tube feeding (AOR: 13.16, 95% confidence interval [CI]: 3.22-53.77), loss of appetite (AOR: 8.15, 95% CI: 4.71-14.12), unemployment (AOR: 4.26, 95% CI: 1.64-11.12), wheelchair-bound (AOR: 2.23, 95% CI: 1.22-4.09) and BMI (AOR: 0.87, 95% CI: 0.82-0.93) were found to be significant predictors of malnutrition risk among stroke patients.

    Conclusion: The risk of malnutrition is highly prevalent among post-stroke patients. Routine nutritional screening, identification of risk factors, and continuous monitoring of dietary intake and nutritional status are highly recommended even after the stroke patient is discharged.

    MeSH terms: Appetite; Enteral Nutrition; Humans; Malaysia; Nutritional Status; Patient Discharge; Risk Factors; Unemployment; Wheelchairs; Nutrition Assessment; Body Mass Index; Prevalence; Confidence Intervals; Logistic Models; Odds Ratio; Hand Strength
  7. Konter E, Kueh YC, Kuan G
    Malays J Med Sci, 2020 Jul;27(4):85-96.
    PMID: 32863748 MyJurnal DOI: 10.21315/mjms2020.27.4.8
    Background: The nature of the relationship between passion and courage and its influence on soccer performance has yet to be revealed. Thus, the purpose of this study was to examine passion attributes (i.e. harmonious and obsessive inclinations) among male soccer players in relation to the level of courageous characteristics (i.e. mastery, determination, assertiveness, venturesome and sacrificial behaviour), other demographic and player characteristics.

    Methods: Data were collected from 278 male soccer players aged 13-38 years (mean [M] = 17.42 ± 4.36) with the number of competitive soccer experiences ranging from 1-28 years (M = 7.51 ± 4.23 years). Participants had at least a year of experience in the sport of soccer completed the validated passion scale, sports courage scale and demographic form.

    Results: Analyses revealed that soccer players with higher levels of total courage (P < 0.001), have more experience in soccer (P = 0.011), and their soccer level being professional (P < 0.001) had a significantly higher score in harmonious passion. There was no significant difference in obsessive passion among different level of total courage (P = 0.154). However, soccer players with more experience (P = 0.011) and higher soccer level being professional (P < 0.001) demonstrated a significant higher score in obsessive passion.

    Conclusion: In conclusion, soccer players with higher harmonious and obsessive passionate attributes had higher courage (except for mastery). In addition, the courageous and passionate traits of the soccer players played meaningful roles in indicating individual and performance variables.

    MeSH terms: Adolescent; Adult; Assertiveness; Demography; Emotions; Humans; Male; Soccer; Sports; Young Adult; Courage
  8. Sahaimi MF, Mat Pa MN, Taib F
    Malays J Med Sci, 2020 Jul;27(4):97-107.
    PMID: 32863749 MyJurnal DOI: 10.21315/mjms2020.27.4.9
    Background: Childhood maltreatment is a global problem, for which the International Society for the Prevention of Child Abuse and Neglect (ISPCAN) has developed the Child Abuse Screening Tool-Child, Home Version (ICAST-CH) to obtain data concerning childhood maltreatment. The study aimed to translate the English version of the ICAST-CH into the Malay language and to assess its reliability and validity.

    Methods: The original English version of the ICAST-CH was first translated into the Malay language. Its content and face validity were tested among five independent individuals. A cross-sectional study using the Malay version (ICAST-CH-M) was then conducted with 255 students in a secondary school in Kota Bharu, Kelantan, Malaysia. The data collected was used to examine the instrument's internal consistency and construct validity. The best ICAST-CH-M model was achieved after varimax rotation application.

    Results: The analysis showed that the Malay version of the ICAST-CH had satisfactory internal consistency, with Cronbach's alpha ranging from 0.59-0.77. The exploratory factor analysis confirmed the validity of the underlying constructs into five domains in the Malay version, but they had to be re-classified as 'physical and psychological abuse', 'neglect', 'sexual abuse', 'exposure to domestic violence' and 'exposure to community violence'.

    Conclusion: This study demonstrated that the ICAST-CH-M is satisfactorily reliable and valid for measuring child maltreatment in Malaysia.

    MeSH terms: Child; Child Abuse; Cross-Sectional Studies; Factor Analysis, Statistical; Humans; Language; Malaysia; Rotation; Students; Translating; Reproducibility of Results; Domestic Violence
  9. Kumar D, Rishabh Kumar R, Kujur A, Kumar C, Sunderam S, Kashyap V, et al.
    Malays J Med Sci, 2020 Jul;27(4):108-118.
    PMID: 32863750 DOI: 10.21315/mjms2020.27.4.10
    Background: This study intends to find the growth patterns of selected school children. Globally accepted statistical methods were used to evaluate the data and prepare a growth chart.

    Methods: This cross-sectional study was conducted with school-going children from 16 selected schools of a tribal district in Jharkhand using multistage cluster random sampling. In each selected school, 60 students, 30 boys and 30 girls, were chosen randomly, totaling 960 children (full data was for 935 children only). Growth charts were created using Lambda-Mu-Sigma (LMS) chart maker version 2.5 for height, weight and body mass index (BMI). In the charts, the LMS values with Z scores for each age and respective height and weight for boys and girls were recorded.

    Results: The 468 boys and 467 girls were in the range of 6-14 years of age. Percentile values obtained for the measured heights in centimetres were evaluated and compared with Indian Academy of Pediatrics reference charts for boys and girls for the same age group, and our values were found to be on the lower side. We were able to plot a growth chart of the data set; as the tribal children's ethnicity is different, this growth chart might be used to assess nutritional status.

    Conclusion: We concluded that growth curves for height, weight, and BMI may be used for evaluating children of age 6-14 years in the tribal population. The measures can be a good indicator of their nourishment status and overall growth patterns, which might be indigenous to their ethnicity. A larger sample size of similar tribal populations may give a clearer picture.

    MeSH terms: Adolescent; Body Weight; Child; Cross-Sectional Studies; Ethnic Groups; Female; Humans; Male; Nutritional Status; Only Child; Pediatrics; Students; Body Mass Index; Sample Size; Growth Charts
  10. Heidari M, Ghodusi Borujeni M, Rezaei P, Kabirian Abyaneh S, Heidari K
    Malays J Med Sci, 2020 Jul;27(4):119-129.
    PMID: 32863751 DOI: 10.21315/mjms2020.27.4.11
    Background: Mental disorders are common among the elderly with serious symptoms of depression and social isolation. This study was conducted to investigate the effect of laughter therapy (LT) on depression and quality of life (QOL) of the elderly living in Abadeh nursing homes.

    Methods: This is a controlled semi-experimental study with a pre-test, post-test design. Ninety eligible ones of the elderly living in the Abadeh nursing homes and from July to September 2017, entered the study. Some of the criteria for entering the study include being over 60 years old, orientation, not having blindness and deafness, lack of physical and mental problems. After determining the intervention and control groups, the scale of depression and QOL was administered to the subjects and their scores were collected in the pre-test.

    Results: Most of the study samples were in the intervention (35.55%) and control (37.77%) group in the age range of 60-69 years. In both intervention and control groups, respectively, 31.11% and 68.88% elderly were males and females. The mean scores of depression in the intervention group after LT (M = 2.57) were lower than those before the intervention (M = 6.87) [CI = -5.58-(-3.02)] and also the results of independent t-test showed a statistically significant difference before and after the intervention between the two groups (P < 0.001). The mean score of dimensions of QOL after LT was higher than that before in the intervention and there was a statistically significant difference in all dimensions with paired t-test (P < 0.001).

    Conclusion: Since the implementation of this programme could improve the mental status and QOL of the elderly, this method of therapy can be used as an alternative or complementary model to enhance the health of the elderly.

    MeSH terms: Mental Disorders; Blindness; Depression; Female; Male; Nursing Homes; Physical Examination; Quality of Life; Sampling Studies; Social Isolation; Laughter Therapy; Control Groups; Orientation, Spatial
  11. Ali SA, Begum T, Reza MF, Fadzil NA, Mustafar F
    Malays J Med Sci, 2020 Jul;27(4):130-138.
    PMID: 32863752 DOI: 10.21315/mjms2020.27.4.12
    Background: Research on audiovisual post-attentive integration has been carried out using a variety of experimental paradigms and experimental groups but not yet studied in dyslexia. We investigated post-attentive integration and topographic voltage distribution in children with dyslexia by analysing the P300 event-related potential (ERP) component.

    Methods: We used a 128-child ERP net for the ERP experiment. Two types of stimuli were presented as either congruent or incongruent stimuli. Congruent stimuli included a matching auditory sound with an animal image, whereas incongruent stimuli included unmatched animal sounds. A total of 24 age-matched children were recruited in the control (n = 12) and dyslexia (n = 12) groups. Children pressed button '1' or '2' when presented with congruent or incongruent stimuli, respectively. The P300 amplitudes and latencies with topographic voltage distribution were analysed for both groups.

    Results: The dyslexia group evoked significantly higher P300 amplitudes at the T4 area than the control group. No significant differences were found in cases of P300 latency. Moreover, the dyslexia group demonstrated a higher intensity of P300 voltage distribution in the right parietal and left occipital areas than the control group.

    Conclusion: Post-attentive integration for children with dyslexia is higher and that this integration process implicated the parietal and occipital areas.

    MeSH terms: Cerebral Cortex; Child; Dyslexia; Humans; Sound; Event-Related Potentials, P300; Control Groups
  12. Tan PH, Teng XX, Gan ZY, Tan SQ
    Malays J Med Sci, 2020 Jul;27(4):139-146.
    PMID: 32863753 MyJurnal DOI: 10.21315/mjms2020.27.4.13
    Background: Appendicitis complicated with appendiceal perforation is common among children. The delay in diagnosis of appendicitis is due to children's varied presentations and their difficulty in communicating symptoms. We aimed to identify clinical factors that aid in predicting acute appendicitis (AA) and perforated appendicitis (PA) among children.

    Methods: This retrospective study involved 215 children aged 12 years and below with the initial diagnosis of AA and PA. Clinical factors studied were demographics, presenting symptoms, body temperature on admission (BTOA), white cell count (WCC), absolute neutrophil count (ANC), platelet count and urinalysis. Simple and multiple logistic regressions were used to determine the odds ratio of the statistically significant clinical factors. Results: The mean age of the included children was 7.98 ± 2.37 years. The odds of AA increased by 2.177 times when the age was ≥ 8 years (P = 0.022), 2.380 times when duration of symptoms ≥ 2 days (P = 0.011), 2.447 times with right iliac fossa (RIF) pain (P = 0.007), 2.268 times when BTOA ≥ 38 °C (P = 0.020) and 2.382 times when neutrophil percentage was ≥ 76% (P = 0.045). It decreased by 0.409 times with non-RIF pain (P = 0.007). The odds of PA was increased by 4.672 times when duration of symptoms ≥ 2 days (P = 0.005), 3.611 times when BTOA ≥ 38 °C (P = 0.015) and 3.678 times when neutrophil percentage ≥ 76% (P = 0.016). There was no significant correlation between WCC and ANC with AA and PA.

    Conclusion: Older children with longer duration of symptoms, RIF pain and higher BTOA are more likely to have appendicitis. The risk of appendiceal perforation increases with longer duration of symptoms and higher BTOA.

    MeSH terms: Acute Disease; Adolescent; Appendicitis; Body Temperature; Child; Child, Preschool; Demography; Fever; Humans; Ilium; Leukocyte Count; Neutrophils; Pain; Platelet Count; Retrospective Studies; Logistic Models; Odds Ratio; Urinalysis
  13. Abdul Khaiyom JH
    Malays J Med Sci, 2020 Jul;27(4):147-153.
    PMID: 32863754 MyJurnal DOI: 10.21315/mjms2020.27.4.14
    COVID-19 and the Movement Control Order (MCO) may trigger a 'next wave' of mental health problems. However, the relationship is not linear. Human psychology also has an impact on the outbreak. Thus, proper strategies to manage human psychology, especially mental health, is very important to break the vicious cycle. This article aims to discuss ways to manage mental health using cognitive-behavioural approaches, mindfulness and spirituality. Specific cognitive-behavioural and mindfulness strategies are listed and suggestions to return to the foundation of human existence are discussed. By practising the cognitive-behavioural, mindfulness, and spirituality strategies described, we may enhance our acceptance, optimism and commitment to prepare for a 'new or renewed normal'.
  14. Dixit A, Yadav R, Singh AV
    Malays J Med Sci, 2020 Jul;27(4):154-158.
    PMID: 32863755 DOI: 10.21315/mjms2020.27.4.15
    Severe acute respiratory illness caused by 2019 novel coronavirus (2019-nCoV), officially named severe acute respiratory syndrome coronavirus (SARS-CoV-2) in late December 2019 is an extremely communicable disease. World Health Organization (WHO) declared coronavirus disease 2019 (COVID-19) as a pandemic as it has spread to at least 200 countries in a short span of time. Being a new disease there is lack of information about pathogenesis and proliferation pathways of this new coronavirus. Currently there is no effective treatment for coronavirus infection; major effort is to develop vaccine against the virus and development of therapeutic drugs for the disease. The development of genome-based vaccine and therapeutic antibodies require thorough testing for safety and will be available after some time. In the meanwhile, the available practical approach is to repurpose existing therapeutic agents, with proven safety record as a rapid response measure for the current pandemic. Here we discuss the presently used repurposed drugs for COVID-19 and the potential for ivermectin (IVM) to be used as a therapeutic option in COVID-19.
    MeSH terms: Communicable Diseases; DNA Viruses; Pharmaceutical Preparations; Humans; Ivermectin; Vaccines; World Health Organization; Coronavirus Infections; Cell Proliferation; Pandemics; Middle East Respiratory Syndrome Coronavirus
  15. Lemin AS, Rahman MM, Pangarah CA, Kiyu A
    J Family Reprod Health, 2020 Mar;14(1):45-51.
    PMID: 32863838
    Objective: Voluntary HIV testing is one of the key strategies in the HIV/AIDS prevention and control program. New National Strategic Plan for 2016-2030 in Malaysia, adapt three zeros viz. 'zero new infections of HIV/AIDS, zero discrimination and zero AIDS-related deaths'. This study aimed to determine the predictors of voluntary HIV testing in Sarawak. Materials and methods: A cross-sectional study was conducted with a total of 900 respondents (450 males and 450 females) from the state of Sarawak, aged 18 years and above, who were selected by gender-stratified multistage cluster sampling. Data were obtained by face-to-face interview using a pretested questionnaire. Binary logistic regression analysis was done to determine the potential predictors for voluntary HIV testing. Results: The prevalence of HIV testing was higher among female respondents (26%) compared to male respondents (14.2%), and the difference was statistically significant (p < 0.001). Binary logistic regression analysis revealed that household income more than MYR 1501 (p = 0.009), not living with a partner (p < 0.001) and discussion on HIV/AIDS (p = 0.019) appeared to be predictors for the male respondents, while, ethnicity was statistically significant for female respondents (p < 0.001). Conclusion: The utilisation of HIV testing was low in both males and females. Thus, the finding of this study could be considered when designing HIV education and screening program in Sarawak.
    MeSH terms: Acquired Immunodeficiency Syndrome; Adolescent; Cross-Sectional Studies; Ethnic Groups; Female; HIV; Humans; Malaysia; Male; Mass Screening; Surveys and Questionnaires; Sexual Partners; Prevalence; Logistic Models
  16. Zhou X, Li Y, Wang W, Wang S, Hou J, Zhang A, et al.
    Theranostics, 2020;10(21):9443-9457.
    PMID: 32863938 DOI: 10.7150/thno.46078
    Objective: Esophageal squamous cell carcinoma (ESCC) is one of the most commonly diagnosed cancer types in China. Recent genomic sequencing analysis indicated the over-activation of Hippo/YAP signaling might play important roles for the carcinogenic process and progression for ESCC patients. However, little is known about the molecular mechanisms that controls Hippo signaling activity in ESCC. Our previous studies indicated that PLCE1-an important risk factor for ESCC-linked to ESCC progression through snail signaling, during this period, we found PARK2 was an important downstream target of PLCE1-snail axis. PARK2 was decreased in ESCC human samples, and correlated with good prognosis in ESCC patients. Further research showed that PARK2 could inhibit YAP, which functions as key downstream effectors of the Hippo pathway. Here, we aim to reveal the molecular mechanisms of PARK2 modulated Hippo pathway in ESCC. Methods: To evaluate the function of PARK2 in ESCC, we used a tissue microarray (TMA) of 223 human ESCC patients and immunohistochemistry to analyze the correlation between PARK2 expression and clinicopathologic variables. Depletion of endogenous PARK2 and YAP from ESCC cells using CRISPR/Cas9 technologies. Flow cytometry and EdU cell proliferation assay were used to detect proliferation of ESCC cells. Nude mice subcutaneous injection and Ki-67 staining were used to evaluate tumor growth in vivo. Migration and invasion assays were performed. In addition, lung metastasis models in mice were used to validate the function of PARK2 in vivo. Identification of PARK2 involved in hippo pathway was achieved by expression microarray screening, double immunofluorescence staining and co-immunoprecipitation assays. The RNA-seq analysis results were validated through quantitative real-time PCR (qRT-PCR) analysis. The protein half-life of YAP was analyzed by Cycloheximide assay, and the TEAD activity was detected by Luciferase reporter assays. Results: Clinical sample of ESCC revealed that low PARK2 expression correlated with late tumor stage (P < 0.001), poor differentiation (P < 0.04), lymph node (P < 0.001) and distant metastasis (P = 0.0087). Multivariate Cox proportional regression analysis further revealed that PARK2 expression (P = 0.032) is an independent prognostic factor for the overall survival of ESCC patients. Besides, the immunohistochemistry results showed that PARK2 negatively correlated with YAP protein level (P < 0.001). PARK2 depletion promotes ESCC progression both through Hippo/YAP axis, while PARK2 overexpression suppresses ESCC tumor progression by Hippo signaling. Co-IP and ubiquitination assays revealed that PARK2 could interact with YAP in the cytosol and promotes YAP K48-linked ubiquitination at K90 sites. Conclusion: Clinical sample analysis and mechanistic study have validated PARK2 as a tumor suppressor for ESCC. Multivariate Cox proportional regression analysis further revealed that PARK2 is an independent prognostic factor for the overall survival of ESCC patients. Cellular and molecular mechanisms in this study showed that PARK2 associated with YAP protein in the cytosol, promoted YAP ubiquitination and proteasome-dependent degradation in ESCC cells. Therefore, as a novel modulator for Hippo signaling, modulation of PARK2 activity or gene expression level could be an appealing strategy to treat esophageal.
    MeSH terms: Animals; Cell Differentiation/genetics; Esophageal Neoplasms/genetics*; Esophageal Neoplasms/pathology*; Female; Humans; Immunohistochemistry/methods; Lymph Nodes/pathology; Male; Mice, Inbred BALB C; Mice, Nude; Middle Aged; Prognosis; Signal Transduction/genetics*; Gene Expression Regulation, Neoplastic/genetics; Protein-Serine-Threonine Kinases/genetics*; Disease Progression; Ubiquitin-Protein Ligases/genetics*; Adaptor Proteins, Signal Transducing/genetics; Cell Proliferation/genetics; Mice; Ubiquitination/genetics
  17. Noviany N, Samadi A, Yuliyan N, Hadi S, Aziz M, Purwitasari N, et al.
    Phytochem Lett, 2020 Feb;35:211-215.
    PMID: 32863985 DOI: 10.1016/j.phytol.2019.12.008
    A new 2-arylbenzofuran, sesbagrandiflorain C (1), together with four known compounds, 2-(3,4-dihydroxy-2-methoxyphenyl)-4-hydroxy-6-methoxybenzofuran-3-carbaldehyde (2), 2-(4-hydroxy-2-methoxyphenyl)-5,6-dimethoxybenzofuran-3-carboxaldehyde (3), sesbagrandiflorain A (4) and sesbagrandiflorain B (5), have been isolated from the stem bark of an Indonesian plant, Sesbania grandiflora (L.) Pers. The chemical structure of compound 1 was elucidated by UV, IR, MS, and NMR spectroscopic techniques. The proton and carbon NMR resonances of 1 were also compared with the predicted chemical shifts obtained from DFT quantum mechanical calculations with Gaussian. None of the compounds showed antibacterial activity against Bacillus subtilis, Escherichia coli, Mycobacterium smegmatis, Pseudomonas aeruginosa, and Staphylococcus aureus in an agar diffusion assay. However, sesbagrandiflorains A (4) and B (5) exhibited moderate activity against Mycobacterium tuberculosis H37Rv. In addition, compounds 1 - 5 have moderate cytotoxicity against HeLa, HepG2, and MCF-7 cancer cell lines.
    MeSH terms: Agar; Anti-Bacterial Agents; Bacillus subtilis; Carbon; Escherichia coli; Humans; Indonesia; Mycobacterium tuberculosis; Protons; Pseudomonas aeruginosa; Staphylococcus aureus; Mycobacterium smegmatis; Plant Bark; Sesbania; MCF-7 Cells
  18. Cheong XK, Yu-Lin Ban A, Abdul Hamid MF
    Respirol Case Rep, 2020 Oct;8(7):e00648.
    PMID: 32864139 DOI: 10.1002/rcr2.648
    Pleural infection is an important clinical problem with significant morbidity. In poorly draining complex pleural effusions, the current management favours a less invasive image-guided placement of smaller bore catheters and adjunctive intrapleural fibrinolysis therapy (IPFT). We describe our experience of using IPFT in three patients with different bleeding risks with complex pleural effusions. The first was a 30-year-old with transfusion-dependent β-thalassemia with haemoglobin of 7.8 g/dL; second was an 87-year-old on dabigatran with haemoglobin of 10 g/dL; and the third was an 80-year-old with diffuse large B-cell lymphoma with haemoglobin of 8.6 g/dL. All three patients received three doses of alteplase and deoxyribonuclease (DNase) without any adverse effects of bleeding and had resolution of the effusion. This case series is an addition to the current literature on the safety of IPFT and we highlight the use of IPFT in patients with low baseline haemoglobin and on anticoagulation therapy.
  19. Ong SL, Bajuri MY, Abdul Suki MH, Nadira F, Zamri KS
    Cureus, 2020 Jul 29;12(7):e9451.
    PMID: 32864272 DOI: 10.7759/cureus.9451
    Hypertrophic scar formation is a major clinical problem that results in both cosmetic issues and functional loss. The management of a hypertrophic scar varies according to the severity of the sequelae from the scar. We describe a method of treatment in a patient who had a history of multiple debridements due to snake bite resulting in severe contracture of the fourth toe complicated with a hypertrophic scar.
  20. Srinivasan S, Treacy R, Herrero T, Olsen R, Leonardo TR, Zhang X, et al.
    Cell Rep Med, 2020 05 19;1(2).
    PMID: 32864636 DOI: 10.1016/j.xcrm.2020.100013
    Development of effective prevention and treatment strategies for pre-eclampsia is limited by the lack of accurate methods for identification of at-risk pregnancies. We performed small RNA sequencing (RNA-seq) of maternal serum extracellular RNAs (exRNAs) to discover and verify microRNAs (miRNAs) differentially expressed in patients who later developed pre-eclampsia. Sera collected from 73 pre-eclampsia cases and 139 controls between 17 and 28 weeks gestational age (GA), divided into separate discovery and verification cohorts, are analyzed by small RNA-seq. Discovery and verification of univariate and bivariate miRNA biomarkers reveal that bivariate biomarkers verify at a markedly higher rate than univariate biomarkers. The majority of verified biomarkers contain miR-155-5p, which has been reported to mediate the pre-eclampsia-associated repression of endothelial nitric oxide synthase (eNOS) by tumor necrosis factor alpha (TNF-α). Deconvolution analysis reveals that several verified miRNA biomarkers come from the placenta and are likely carried by placenta-specific extracellular vesicles.
External Links