Browse publications by year: 2020

  1. Amjath-Babu TS, Krupnik TJ, Thilsted SH, McDonald AJ
    Food Secur, 2020;12(4):761-768.
    PMID: 32837652 DOI: 10.1007/s12571-020-01083-2
    In the context of developing countries, early evidence suggests that the impacts of the COVID-19 pandemic on food production systems is complex, heterogenous, and dynamic. As such, robust monitoring of the impact of the health crisis and containment measures across agricultural value chains will likely prove vitally important. With Bangladesh as a case study, we discuss the building blocks of a comprehensive monitoring system for prioritizing and designing interventions that respond to food system disruptions from COVID-19 and preemptively avoid further cascading negative effects. We also highlight the need for parallel research that identifies pathways for enhancing information flow, analysis, and action to improve the efficiency and reliability of input and output value chains. In aggregate, this preliminary work highlights the building blocks of resilient food systems to external shocks such as COVID-19 pandemic in the context of developing nations. In doing so, we call attention to the importance of 'infection safe' agricultural input and output distribution logistics, extended social safety nets, adequate credit facilities, and innovative labor management tools alongside, appropriate farm mechanization. In addition, digital extension services, circular nutrient flows, enhanced storage facilities, as well as innovative and robust marketing mechanisms are required. These should be considered in parallel with effective international trade management policies and institutions as crucial supportive measures.
  2. Tang KHD
    Z Gesundh Wiss, 2020 Jun 13.
    PMID: 32837842 DOI: 10.1007/s10389-020-01316-w
    Aim: The first Covid-19 cases were reported in Malaysia on 25 January 2019 followed by a quiescent period before an upward swing of the cases at the end of February 2020, partly attributed to mass gathering during a religious event. This short communication aims to provide an overview of the measures taken by the Malaysian Government in response to Covid-19, and of the effectiveness of the Movement Control Order.

    Subjects and methods: This short communication reviews articles and government announcements related to containment measures and the Movement Control Order of Malaysia, and graphically presents data pertinent to Covid-19 in Malaysia in order to show the relationship between fluctuations in Covid-19 cases and movement control.

    Results: At the onset of the Covid-19 outbreak, Malaysia had initiated travel restrictions and quarantine; but with a persistent increase in new Covid-19 cases, the Movement Control Order was finally rolled out on 18 March 2020, requiring closure of all businesses except those providing essential services and items. Enforcement of the order was tightened progressively, resulting in significant improvement of compliance, while other interventions such as tracking of potential contacts and medical screening were underway, and the media continued to provide updates and general advices. The numbers of new and active Covid-19 cases started showing evident downtrends from mid-April, thus indicating the effectiveness of movement control and its compliance.

    Conclusion: The article provides insight into crucial factors contributing to the success of movement control to effectively contain Covid-19, and highlights the need to prevent future upsurge through continuous monitoring and enforcement.

    MeSH terms: Communication; Communications Media; Disease Outbreaks; Government; Humans; Malaysia; Quarantine; Travel
  3. Al-Okaily M, Alqudah H, Matar A, Lutfi A, Taamneh A
    Data Brief, 2020 Aug 18.
    PMID: 32837976 DOI: 10.1016/j.dib.2020.106176
    The COVID-19 pandemic has produced an unprecedented change in the educational system worldwide. Besides the economic and social impacts, there is a dilemma of accepting the new educational system "e-learning" by students within educational institutions. In particular, universities students have to handle several kinds of environmental, electronic and mental struggles due to COVID-19. To catch the current circumstances of more than two hundred thousand Jordanian university student during COVID-19. 2,500 students have been randomly selected to respond on an online survey using universities' portals and websites between March and April 2020. At the end of the data gathering process, we have received 587 records. The dataset includes 1) Demographics of students; 2) students' perspectives concerning the factors influencing their intention to use e-learning system within the Jordanian universities context. Data were analyzed using Partial Least Squares - Structural Equation Modelling (PLS-SEM). Next, the result has confirmed the positive of direct effect variables (subjective norm, perceived ease of use, and perceived usefulness) on the students' intention to use e-learning system. Next, the result has also confirmed the mediating effect of perceived usefulness and perceived ease of use between subjective norm and the behavioral intention to use the e-learning system with partially supported.
    MeSH terms: Computer-Assisted Instruction; Demography; Humans; Learning; Surveys and Questionnaires; Social Change; Students; Universities; Least-Squares Analysis; Intention; Pandemics
  4. Huang L, Liu Z, Li H, Wang Y, Li Y, Zhu Y, et al.
    Geohealth, 2020 Jul 07.
    PMID: 32838101 DOI: 10.1029/2020GH000272
    The outbreak of COVID-19 in China has led to massive lockdowns in order to reduce the spread of the epidemic and control human-to-human transmission. Subsequent reductions in various anthropogenic activities have led to improved air quality during the lockdown. In this study, we apply a widely used exposure-response function to estimate the short-term health impacts associated with PM2.5 changes over the Yangtze River Delta (YRD) region due to COVID-19 lockdown. Concentrations of PM2.5 during lockdown period reduced by 22.9% to 54.0% compared to pre-lockdown level. Estimated PM2.5-related daily premature mortality during lockdown period is 895 (95% confidential interval: 637-1081), which is 43.3% lower than pre-lockdown period and 46.5% lower compared with averages of 2017-2019. According to our calculation, total number of avoided premature death associated PM2.5 reduction during the lockdown is estimated to be 42.4 thousand over the YRD region, with Shanghai, Wenzhou, Suzhou (Jiangsu province), Nanjing, and Nantong being the top five cities with largest health benefits. Avoided premature mortality is mostly contributed by reduced death associated with stroke (16.9 thousand, accounting for 40.0%), ischemic heart disease (14.0 thousand, 33.2%) and chronic obstructive pulmonary disease (7.6 thousand, 18.0%). Our calculations do not support or advocate any idea that pandemics produce a positive note to community health. We simply present health benefits from air pollution improvement due to large emission reductions from lowered human and industrial activities. Our results show that continuous efforts to improve air quality are essential to protect public health, especially over city-clusters with dense population.
    MeSH terms: Air Pollution; China; Cities; Communicable Disease Control; Humans; Public Health; Myocardial Ischemia; Stroke; Pulmonary Disease, Chronic Obstructive; Rivers; Particulate Matter; Pandemics; Mortality, Premature
  5. Wong TC, Lee ZY, Sia TLL, Chang AKW, Chua HH
    SN Compr Clin Med, 2020 Aug 15.
    PMID: 32838193 DOI: 10.1007/s42399-020-00443-5
  6. Cheong MWL
    PMID: 32838225 DOI: 10.1002/jac5.1315
    Introduction: The Coronavirus disease 2019 (COVID-19) pandemic has significantly affected health care systems around the world. In many hospitals and health care facilities, services and health care workers have been reorganized and restructured to meet the demands of the pandemic. The impact of the pandemic on hospital-based clinical pharmacists and their ability to deliver pharmaceutical care is currently unknown.

    Objective: This study aimed to explore the impact of the COVID-19 pandemic on hospital-based clinical pharmacists working in Malaysia and the implications on how clinical pharmacy is perceived as a health care service.

    Methods: A qualitative study was designed to meet the research objectives. Nineteen hospital-based clinical pharmacists consented and participated in one-on-one, semi-structured interviews. The interviews were transcribed and analyzed using an iterative thematic analysis approach.

    Results: The experiences and views of the participants were reported. Three main themes were developed: 'Reassignment and other changes in clinical pharmacist roles', 'Adapting clinical pharmacy services to COVID-19', and 'The need for clinical pharmacists in the ward'. The findings indicate that in many cases, clinical pharmacy services were fully or partially withdrawn from the ward to reduce the risk of infection and to conserve the usage of personal protective equipment. Despite this, clinical pharmacists continued to support patient care in hospitals through the use of technology. The withdrawal of clinical pharmacy services, however, raises concern that the role of clinical pharmacists is still poorly recognized.

    Conclusion: Clinical pharmacists in hospitals continue to support patient care despite the disruption caused by the COVID-19 pandemic. Greater support and recognition of their role is required in order to empower and enhance their ability to deliver pharmaceutical care.This article is protected by copyright. All rights reserved.

    MeSH terms: Personal Protective Equipment; Copyright; Delivery of Health Care; Patient Care; Humans; Malaysia; Pharmacists; Pharmacy Service, Hospital; Coronavirus; Coronavirus Infections; Pandemics
  7. Mustafa MA, Fauzi MH, Abu Bakar MA, Mohd Noor N, Mohamed Hussain M
    Ann Emerg Med, 2020 Sep;76(3):362-378.
    PMID: 32828330 DOI: 10.1016/j.annemergmed.2020.03.024
  8. Sultan T, Cheah CW, Ibrahim NB, Asif MK, Vaithilingam RD
    J Dent, 2020 Oct;101:103455.
    PMID: 32828845 DOI: 10.1016/j.jdent.2020.103455
    OBJECTIVES: This clinical study assessed and compared the linear and volumetric changes of extraction sockets grafted with a combination of Platelet-Rich Fibrin (PRF) and Calcium Sulfate (CS) (PRF-CS), and extraction sockets grafted with a combination of PRF and xenograft (X) (PRF-X).

    METHODS: Five single maxillary premolar extraction sockets received PRF-CS grafts and five single maxillary premolar sockets received PRF-X grafts. Linear (horizontal and vertical) measurements were accomplished using Cone Beam Computed Tomography (CBCT) images and volumetric changes were assessed using MIMICS software. Soft tissue level changes were measured using Stonecast models. All measurements were recorded at baseline (before extraction) and at 5-months post-extraction.

    RESULTS: Significant reduction in vertical and horizontal dimensions were observed in both groups except for distal bone height (DBH = 0.44 ± 0.45 mm, p = 0.09) and palatal bone height (PBH = 0.39 ± 0.34 mm, p = 0.06) in PRF-X group. PRF-CS group demonstrated mean horizontal shrinkage of 1.27 ± 0.82 mm (p = 0.02), when compared with PRF-X group (1.40 ± 0.85 mm, p = 0.02). Vertical resorption for mesial bone height (MBH = 0.56 ± 0.25 mm, p = 0.008), buccal bone height (BBH = 1.62 ± 0.91 mm, p = 0.01) and palatal bone height (PBH = 1.39 ± 0.87 mm, p = 0.02) in PRF-CS group was more than resorption in PRF-X group (MBH = 0.28 ± 0.14 mm, p = 0.01, BBH = 0.63 ± 0.39 mm, p = 0.02 and PBH = 0.39 ± 0.34 mm, p = 0.06). Volumetric bone resorption was significant within both groups (PRF-CS = 168.33 ± 63.68 mm3, p = 0.004; PRF-X = 102.88 ± 32.93 mm3, p = 0.002), though not significant (p = 0.08) when compared between groups. In PRF-X group, the distal soft tissue level (DSH = 1.00 ± 0.50 mm, p = 0.03) demonstrated almost 2 times more reduction when compared with PRF-CS group (DSH = 1.00 ± 1.00 mm, 0.08). The reduction of the buccal soft tissue level was pronounced in PRF-CS group (BSH = 2.00 ± 2.00 mm, p = 0.06) when compared with PRF-X group (BSH = 1.00 ± 1.50 mm, p = 0.05).

    CONCLUSIONS: PRF-CS grafted sites showed no significant difference with PRF-X grafted sites in linear and volumetric dimensional changes and might show clinical benefits for socket augmentation. The study is officially registered with ClinicalTrials.gov Registration (NCT03851289).

    MeSH terms: Bicuspid; Bone Resorption; Calcium Sulfate; Mouth; Software; Transplantation, Heterologous; Tooth Socket; Cone-Beam Computed Tomography; Spiral Cone-Beam Computed Tomography; Heterografts
  9. Ahmed A, Ijaz M, Ghauri HN, Aziz MU, Ghaffar A, Naveed M, et al.
    PMID: 32829184 DOI: 10.1016/j.cimid.2020.101524
    Feline anaplasmosis is considered as an emerging tick-borne disease of zoonotic potential. The aim of current study was to investigate the molecular prevalence of anaplasmosis, associated risk factors, and alterations in hematological parameters of domestic cats from Lahore, Pakistan. Blood samples of 100 domestic cats from district Lahore were examined microscopically and the extracted genomic DNA from each sample was processed for the amplification of 16 S rRNA gene of Anaplasma. PCR confirmed isolates were purified for sequencing. The data regarding the risk factors was collected in a predesigned questionnaire and statistically analyzed by logistic regression analysis. The study found a molecular prevalence of 13% (13/100) among analyzed blood samples. The nucleotide analysis of Anaplasmataceae species sequences amplified by PCR showed high resemblance (99%) with isolates from Korea, Japan, Malaysia, Philippines, and India. The potential risk factors found to be significantly associated (p 
    MeSH terms: Anaplasma/genetics; Animals; Cats; DNA, Bacterial/genetics; Pakistan/epidemiology; Phylogeny; RNA, Ribosomal, 16S/genetics
  10. Lam SS, Chew KW, Show PL, Ma NL, Ok YS, Peng W, et al.
    Environ Res, 2020 11;190:109966.
    PMID: 32829186 DOI: 10.1016/j.envres.2020.109966
    Two of the world most endangered marine and terrestrial species are at the brink of extinction. The vaquita (Phocoena sinus) is the smallest existing cetacean and the population has declined to barely 22 individuals now remaining in Mexico's Gulf of California. With the ongoing decline, it is likely to go extinct within few years. The primary threat to this species has been mortality as a result of by-catch from gillnet fishing as well as environmental toxic chemicals and disturbance. This has called for the need to establish a National Park within the Gulf of California to expand essential habitat and provide the critical ecosystem protection for vaquita to thrive and multiply, given that proper conservation enforcement and management of the park are accomplished. In the terrestrial environment, the cheetah (Acinonyx jubatus) is reduced to a low number worldwide with the Iran subpopulation currently listed as Critically Endangered and the Indian subpopulation already extinct. There is a need for conservation efforts due to habitat loss, but also an indication of the conspicuous threat of illegal trade and trafficking from Africa and Arab countries in the Middle East. Funds have also been set up to provide refuges for the cheetah by working directly with farmers and landowners, which is a critical movement in adaptive management. These are the potential options for the preservation and possibly the expansion of the overall vaquita and cheetah populations.
    MeSH terms: Africa; Animals; Acinonyx*; Conservation of Natural Resources; Humans; Iran; Middle East; Ecosystem; Endangered Species
  11. Charles Shapu R, Ismail S, Ahmad N, Lim PY, Abubakar Njodi I
    Nutrients, 2020 Aug 13;12(8).
    PMID: 32823548 DOI: 10.3390/nu12082426
    Adolescence is a phase in the life cycle of human beings. Adequate knowledge, attitudes and practices towards malnutrition are necessary for proper growth and development and for their future children. This systematic review aimed to determine the effect of health education intervention to improve the knowledge, attitudes and practices of adolescents on malnutrition. PubMed, Scopus, clinical trials, CINAHL, SAGE, Science Direct and Medline were searched according to Preferred Reporting Item for Systematic Reviews and Meat-analysis (PRISMA) guidelines to identified published studies from January 2013 to December 2019 based on the inclusion and exclusion criteria. A total of eight studies were included in this review. Data extraction was done based on randomized controlled trial only. Three out of the eight studies had low risk of bias, the overall evidence of the study was moderate. Findings from this study suggest that health education intervention among adolescents have significantly improved their knowledge, attitudes and practices. More specific interventions should be conducted in low and middle income countries since they bear more of the burden of malnutrition globally.
    MeSH terms: Adolescent; Adolescent Behavior/psychology*; Feeding Behavior/psychology*; Female; Health Education/methods*; Humans; Health Knowledge, Attitudes, Practice*; Male; Malnutrition/psychology*
  12. Mathialagan RD, Abd Hamid Z, Ng QM, Rajab NF, Shuib S, Binti Abdul Razak SR
    PMID: 32823552 DOI: 10.3390/ijerph17165865
    Hematopoietic stem/progenitor cells (HSPCs) are susceptible to benzene-induced genotoxicity. However, little is known about the mechanism of DNA damage response affecting lineage-committed progenitors for myeloid, erythroid, and lymphoid. Here, we investigated the genotoxicity of a benzene metabolite, 1,4-benzoquinone (1,4-BQ), in HSPCs using oxidative stress and lineage-directed approaches. Mouse bone marrow cells (BMCs) were exposed to 1,4-BQ (1.25-12 μM) for 24 h, followed by oxidative stress and genotoxicity assessments. Then, the genotoxicity of 1,4-BQ in lineage-committed progenitors was evaluated using colony forming cell assay following 7-14 days of culture. 1,4-BQ exposure causes significant decreases (p < 0.05) in glutathione level and superoxide dismutase activity, along with significant increases (p < 0.05) in levels of malondialdehyde and protein carbonyls. 1,4-BQ exposure induces DNA damage in BMCs by significantly (p < 0.05) increased percentages of DNA in tail at 7 and 12 μM and tail moment at 12 μM. We found crucial differences in genotoxic susceptibility based on percentages of DNA in tail between lineage-committed progenitors. Myeloid and pre-B lymphoid progenitors appeared to acquire significant DNA damage as compared with the control starting from a low concentration of 1,4-BQ exposure (2.5 µM). In contrast, the erythroid progenitor showed significant damage as compared with the control starting at 5 µM 1,4-BQ. Meanwhile, a significant (p < 0.05) increase in tail moment was only notable at 7 µM and 12 µM 1,4-BQ exposure for all progenitors. Benzene could mediate hematological disorders by promoting bone marrow oxidative stress and lineage-specific genotoxicity targeting HSPCs.
    MeSH terms: Animals; Bone Marrow*; Bone Marrow Cells; Benzoquinones/toxicity; Oxidative Stress*; Mice
  13. Ganesan T, Sinniah A, Chik Z, Alshawsh MA
    Nutrients, 2020 Aug 13;12(8).
    PMID: 32823596 DOI: 10.3390/nu12082430
    Punicalagin (PU), a polyphenol extracted from pomegranate (Punica granatum) husk is proven to have anti-cancer effects on different types of cancer including colorectal cancer (CRC). Its role in modulating endogenous protein as a means of eliciting its anti-cancer effects, however, has not been explored to date. Hence, this study aimed to investigate the role of PU in modulating the interplay between apoptosis and autophagy by regulating Annexin A1 (Anx-A1) expression in HCT 116 colorectal adenocarcinoma cells. In the study, selective cytotoxicity, pro-apoptotic, autophagic and Anx-A1 downregulating properties of PU were shown which indicate therapeutic potential that this polyphenol has against CRC. Autophagy flux analysis via flow cytometry showed significant autophagosomes degradation in treated cells, proving the involvement of autophagy. Proteome profiling of 35 different proteins in the presence and absence of Anx-A1 antagonists in PU-treated cells demonstrated a complex interplay that happens between apoptosis and autophagy that suggests the possible simultaneous induction and inhibition of these two cell death mechanisms by PU. Overall, this study suggests that PU induces autophagy while maintaining basal level of apoptosis as the main mechanisms of cytotoxicity via the modulation of Anx-A1 expression in HCT 116 cells, and thus has a promising translational potential.
    MeSH terms: Adenocarcinoma/drug therapy*; Antineoplastic Agents/pharmacology*; Humans; Plant Extracts/pharmacology*; Colorectal Neoplasms/drug therapy*; Annexin A1/drug effects; HCT116 Cells; Hydrolyzable Tannins/pharmacology*
  14. Chew SS, Tan LT, Law JW, Pusparajah P, Goh BH, Ab Mutalib NS, et al.
    Cancers (Basel), 2020 Aug 13;12(8).
    PMID: 32823729 DOI: 10.3390/cancers12082272
    Colorectal cancer (CRC) is a global public health issue which poses a substantial humanistic and economic burden on patients, healthcare systems and society. In recent years, intestinal dysbiosis has been suggested to be involved in the pathogenesis of CRC, with specific pathogens exhibiting oncogenic potentials such as Fusobacterium nucleatum, Escherichia coli and enterotoxigenic Bacteroides fragilis having been found to contribute to CRC development. More recently, it has been shown that initiation of CRC development by these microorganisms requires the formation of biofilms. Gut microbial biofilm forms in the inner colonic mucus layer and is composed of polymicrobial communities. Biofilm results in the redistribution of colonic epithelial cell E-cadherin, increases permeability of the gut and causes a loss of function of the intestinal barrier, all of which enhance intestinal dysbiosis. This literature review aims to compile the various strategies that target these pathogenic biofilms and could potentially play a role in the prevention of CRC. We explore the potential use of natural products, silver nanoparticles, upconverting nanoparticles, thiosalicylate complexes, anti-rheumatic agent (Auranofin), probiotics and quorum-sensing inhibitors as strategies to hinder colon carcinogenesis via targeting colon-associated biofilms.
    MeSH terms: Gastrointestinal Microbiome; Auranofin; Bacteroides fragilis; Biological Products; Escherichia coli; Humans; Public Health; Silver; Colorectal Neoplasms; Cadherins; Fusobacterium nucleatum; Cost of Illness; Probiotics; Metal Nanoparticles; Dysbiosis
  15. Ganesan T, Sinniah A, Ibrahim ZA, Chik Z, Alshawsh MA
    Molecules, 2020 Aug 14;25(16).
    PMID: 32823805 DOI: 10.3390/molecules25163700
    Annexin A1 has been extensively investigated as an anti-inflammatory protein, but its role in different types of cancer has not been consolidated in a single systematic review to date. Thus, the aim of this paper is to systematically review and critically analyse 18 studies (in-vivo and in-vitro) to consolidate, in a concerted manner, all the information on differential expression of Annexin A1 in different types of cancer and the role this protein plays in tumorigenesis. Pubmed, Scopus, Web of Science, and ScienceDirect were used for the literature search and the keywords used are "annexin A1," "lipocortin 1," "cancer," "malignancy," "neoplasm," "neoplasia," and "tumor." A total of 1128 articles were retrieved by implementing a standard search strategy subjected to meticulous screening processes and 442 articles were selected for full article screening. A total of 18 articles that adhered to the inclusion criteria were included in the systematic review and these articles possessed low to moderate bias. These studies showed a strong correlation between Annexin A1 expression and cancer progression via modulation of various cancer-associated pathways. Differential expression of Annexin A1 is shown to play a role in cellular proliferation, metastasis, lymphatic invasion, and development of resistance to anti-cancer treatment. Meta-analysis in the future may provide a statistically driven association between Annexin A1 expression and malignancy progression.
    MeSH terms: Animals; Humans; Neoplasms/genetics; Neoplasms/metabolism; Neoplasms/pathology*; Biomarkers, Tumor/genetics; Biomarkers, Tumor/metabolism*; Signal Transduction; Gene Expression Regulation, Neoplastic*; Apoptosis*; Annexin A1/genetics; Annexin A1/metabolism*
  16. Haida Z, Nakasha JJ, Hakiman M
    Plants (Basel), 2020 Aug 14;9(8).
    PMID: 32823824 DOI: 10.3390/plants9081030
    Clinacanthus nutans, commonly known as Sabah snake grass, is one of the more important medicinal plants in Malaysia's herbal industry. C. nutans has gained the attention of medical practitioners due to its wide range of bioactive compounds responsible for various biological activities, such as anti-cancer, anti-venom and anti-viral activities. Due to its high pharmacological properties, the species has been overexploited to meet the demands of the pharmaceutical industry. The present study was conducted to establish a suitable in vitro culture procedure for the mass propagation of C. nutans. Murashige and Skoog (MS) basal medium, supplemented with different types of cytokinins, auxins, basal medium strength and sucrose concentrations, were tested. Based on the results, a full-strength MS basal medium supplemented with 12 µM 6-benzylaminopurine (BAP) and 30 g/L sucrose was recorded as the best outcome for all the parameters measured including the regeneration percentage, number of shoots, length of shoots, number of leaves and fresh weight of leaves. In the analysis of the phenolics content and antioxidant activities, tissue-cultured leaf extracts assayed at 100 °C exhibited the highest phenolic content and antioxidant activities. The propagation of C. nutans via a plant tissue culture technique was recorded to be able to produce high phenolic contents as well as exhibit high antioxidant activities.
    MeSH terms: Antioxidants; Antivenins; Benzyl Compounds; Cytokinins; Indoleacetic Acids; Malaysia; Phenols; Plants, Medicinal; Purines; Sucrose; Tissue Extracts; Venoms; Acanthaceae; Tissue Culture Techniques
  17. O'Rielly R, Li H, Lim SM, Yazbeck R, Kritas S, Ullrich SS, et al.
    Nutrients, 2020 Aug 14;12(8).
    PMID: 32823899 DOI: 10.3390/nu12082446
    Chronic isoleucine supplementation prevents diet-induced weight gain in rodents. Acute-isoleucine administration improves glucose tolerance in rodents and reduces postprandial glucose levels in humans. However, the effect of chronic-isoleucine supplementation on body weight and glucose tolerance in obesity is unknown. This study aimed to investigate the impact of chronic isoleucine on body weight gain and glucose tolerance in lean and high-fat-diet (HFD) induced-obese mice. Male C57BL/6-mice, fed a standard-laboratory-diet (SLD) or HFD for 12 weeks, were randomly allocated to: (1) Control: Drinking water; (2) Acute: Drinking water with a gavage of isoleucine (300 mg/kg) prior to the oral-glucose-tolerance-test (OGTT) or gastric-emptying-breath-test (GEBT); (3) Chronic: Drinking water with 1.5% isoleucine, for a further six weeks. At 16 weeks, an OGTT and GEBT was performed and at 17 weeks metabolic monitoring. In SLD- and HFD-mice, there was no difference in body weight, fat mass, and plasma lipid profiles between isoleucine treatment groups. Acute-isoleucine did not improve glucose tolerance in SLD- or HFD-mice. Chronic-isoleucine impaired glucose tolerance in SLD-mice. There was no difference in gastric emptying between any groups. Chronic-isoleucine did not alter energy intake, energy expenditure, or respiratory quotient in SLD- or HFD-mice. In conclusion, chronic isoleucine supplementation may not be an effective treatment for obesity or glucose intolerance.
    MeSH terms: Animals; Body Weight; Energy Intake; Energy Metabolism; Gastric Emptying; Glucose; Glucose Tolerance Test; Humans; Isoleucine; Male; Mice, Inbred C57BL; Mice, Obese; Obesity; Rodentia; Weight Gain; Glucose Intolerance; Dietary Supplements; Mice; Drinking Water
  18. Ibrahim MI, Zubair IU, Shafei MN, Ahmad MI, Yaacob NM
    PMID: 32824040 DOI: 10.3390/ijerph17165916
    The prevalence of low back pain (LBP) among nurses is high. The main aim of this study was to evaluate the effectiveness of an interactive LBP module based on the Back School Program in improving Oswestry Disability Scores (ODSs) among nurses in government hospitals in Penang, Malaysia. A cluster-randomized experimental study was conducted within four public hospitals. These hospitals were randomized to intervention and control groups. A total of 284 nurses from the selected hospitals were randomly selected (142 in each group). An interactive LBP intervention module based on the Back School Program was designed and prescribed. Both the intervention and control groups were assessed using the Oswestry Disability Questionnaire at baseline and at the end of the third and sixth weeks. Out of 284 participants, 281 completed this study. A between-group comparison revealed that ODSs were significantly lower in the intervention group than in the control group at the ends of the third (p = 0.006) and sixth weeks (p < 0.001). Within-group changes revealed a significant reduction in ODSs within the intervention group from baseline to the third (p < 0.001) and sixth weeks (p < 0.001) of the intervention. This simple interactive LBP module was effective in reducing symptoms of LBP among nurses as early as three weeks, and this effect was sustained until the sixth week of the intervention.
    MeSH terms: Child; Female; Hospitals, Public; Humans; Malaysia/epidemiology; Male; Surveys and Questionnaires; Schools; Low Back Pain/epidemiology; Low Back Pain/prevention & control*
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