Browse publications by year: 2023

  1. Buyong NL, Nillian E
    Food Sci Nutr, 2023 Oct;11(10):6052-6059.
    PMID: 37823107 DOI: 10.1002/fsn3.3541
    This study aimed to investigate the physiochemical properties of Sarawak's adapted Liberica coffee silverskin (CS) using multiple solvents (distilled water, methanol, and ethanol) and its impact on the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities of the CS. The results showed that the highest TPC was observed in the methanol extract (15.24 ± 0.65 mg GAE/g), while the highest TFC was recorded when extracted with ethanol (25.14 ± 0.59 mg QE/g). The DPPH activity was also found to be highest in the ethanol extract (83.85 ± 1.78%), concurred by the results in the FRAP assay as the highest reduction was also in ethanol (11.40 ± 18.57 μmol FSE/g). These findings demonstrate that the bioactive compounds of CS extracted can be greatly influenced by the choice of solvent while highlighting the potential for Sarawak's adapted Liberica CS to be further harnessed into a value-added product and enabling a better by-product waste management.
  2. Lee HF, Hsu HC, Efendi F, Ramoo V, Susanti IA
    PLoS One, 2023;18(10):e0291073.
    PMID: 37816005 DOI: 10.1371/journal.pone.0291073
    The primary frontline healthcare providers who have frequent contact with COVID-19 patients are nurses. Many nurses have been infected with COVID-19 and have experienced severe emotional exhaustion and burnout. It is essential to assess nurses' psychological health during the COVID-19 pandemic. This study aimed to analyze the factors associated with burnout, resilience, and empowerment among Indonesian COVID-19 nurse survivors. In this cross-sectional study, 182 COVID-19 survivor nurses participated from September to November 2022 with convenience sampling. An online survey using the Copenhagen Burnout Inventory (CBI), the Connor-Davidson Resilience Scale (CD-RISC), and the Psychological Empowerment Scale (PES) were used to collect data. The data were analyzed using descriptive and binary logistic regression analyses. The majority of the nurses were aged between 30-45 years (61.6%), and females (67.4%) experienced burnout. Higher resilience was found among nurses contracting COVID-19 (83.1%). In the multivariate logistic regression analysis, the absence of psychological impact (OR = 0.44, 95% CI = 0.21-0.93) is significantly related to higher burnout experience. In addition, workplace, especially in hospital (OR = 4.32, 95% CI = 1.09-17.09) was associated with resilience, and a gap time after receiving negative COVID-19 result (OR = 3.90, 95% CI = 1.27-12.03) was correlated with psychological empowerment, in our results 4-6 month after had a negative result was at higher risk. To maintain a positive psychological aspect for COVID-19 nurse survivors, it needs to implement psychological support in the workplace and ensure an appropriate workload of nurse professionals.
    MeSH terms: Adult; Cross-Sectional Studies; Female; Humans; Indonesia/epidemiology; Middle Aged; Nurses*; Surveys and Questionnaires; Resilience, Psychological*; Pandemics
  3. Iqbal RM, Binti Riza Effendi NI, Syed Alwi SS, Saidi HI, Sarchio SNE
    PLoS One, 2023;18(10):e0283098.
    PMID: 37816038 DOI: 10.1371/journal.pone.0283098
    Rapid outbreak of coronavirus disease 2019 has caused the implementation of the movement control order (MCO) which aimed to reduce the spread in Covid-19 infections. While some may find it easy to adjust to the new norm, others found it difficult to switch from their normal routines and habits as according to the MCO SOP. This resulted in a more frequent insomnia and depression that subsequently impacted their mental health. Insomnia and depression levels are examined in this study as they relate to the Covid-19 Pandemic and the MCO among Malaysian undergraduate health sciences students at the Faculty of Medicine and Health Sciences, UPM. Random sampling methods were utilised with consideration of inclusion and exclusion criteria. The Patient Health Questionnaire-9 (PHQ-9) and the Insomnia Severity Index (ISI) were the instrument packages used in this investigation. An internet platform was used to distribute the questionnaire. Based on the results, it is concluded that depression and insomnia are significantly correlated, with a p-value of 0.05. This study also revealed the link between the severity of insomnia and the severity of depression among UPM students studying health sciences. The percentage of students with depression and insomnia was rather high (54.9% and 33.9%, respectively), and this occurred during the second wave of Covid-19 cases in Malaysia.
    MeSH terms: Depression/epidemiology; Faculty; Humans; Malaysia/epidemiology; Students/psychology; Pandemics
  4. Feng S, Xie X, Liu J, Li A, Wang Q, Guo D, et al.
    J Nanobiotechnology, 2023 Oct 10;21(1):370.
    PMID: 37817254 DOI: 10.1186/s12951-023-02139-z
    Microalgae as the photosynthetic organisms offer enormous promise in a variety of industries, such as the generation of high-value byproducts, biofuels, pharmaceuticals, environmental remediation, and others. With the rapid advancement of gene editing technology, CRISPR/Cas system has evolved into an effective tool that revolutionised the genetic engineering of microalgae due to its robustness, high target specificity, and programmability. However, due to the lack of robust delivery system, the efficacy of gene editing is significantly impaired, limiting its application in microalgae. Nanomaterials have become a potential delivery platform for CRISPR/Cas systems due to their advantages of precise targeting, high stability, safety, and improved immune system. Notably, algal-mediated nanoparticles (AMNPs), especially the microalgae-derived nanoparticles, are appealing as a sustainable delivery platform because of their biocompatibility and low toxicity in a homologous relationship. In addition, living microalgae demonstrated effective and regulated distribution into specified areas as the biohybrid microrobots. This review extensively summarised the uses of CRISPR/Cas systems in microalgae and the recent developments of nanoparticle-based CRISPR/Cas delivery systems. A systematic description of the properties and uses of AMNPs, microalgae-derived nanoparticles, and microalgae microrobots has also been discussed. Finally, this review highlights the challenges and future research directions for the development of gene-edited microalgae.
    MeSH terms: Genetic Engineering; Nanoparticles*; CRISPR-Cas Systems/genetics; Gene Editing
  5. Kashif S, Foong CC
    J Pak Med Assoc, 2023 Sep;73(9):1837-1842.
    PMID: 37817694 DOI: 10.47391/JPMA.7857
    OBJECTIVE: To assess burnout by the extent of exhaustion and disengagement, and to compare the two elements among obstetrics and gynaecology healthcare professionals.

    METHODS: The cross-sectionalstudy was conducted at the Federal Government Polyclinic Hospital, Islamabad, Pakistan, in July and August, 2020 during the coronavirus disease-2019 pandemic, and comprised healthcare professionals from the obstetrics and gynaecology department. Data was collected online using the 25-item Oldenburg Burnout Inventory. Data was analysed using SPSS 26.

    RESULTS: Ofthe 142 individuals approached, 102(71.83%)responded; 55(53.92%) doctors, 43(42.15%) nurses and 4(3.92%) operation theatre technicians. Overall, there were 98(96.1%) females, and 67(65.7%) married subjects. Mean exhaustion scorewas 2.53±0.54 anddisengagementscorewas 2.14±0.57. Burnoutwasfoundin40(39.2%)participants, 83 (81.4%)were exhausted, 44(43.1%)weredisengaged, and4(3.1%)weredisengagedbutnot exhausted. Participants aged>50 yearswere significantly more exhausted and disengaged than the younger ones(p<0.05). Those with work hours >60 per week were significantly more exhausted and disengaged than the rest (p<0.05).

    CONCLUSION: Health care professionals inobstetrics and gynaecology teams showed considerably highburnout levels during the coronavirus disease-2019 pandemic in Pakistan.

    MeSH terms: Cross-Sectional Studies; Delivery of Health Care; Female; Gynecology*; Health Personnel; Hospitals, Public; Humans; Male; Pakistan/epidemiology
  6. Adilah Harun N, Ramli N, Rahman Kazim Azli A, Hassan H, Bariah Chi Adam K, Haikal Muhamad Halil M
    Saudi Dent J, 2023 Sep;35(6):663-667.
    PMID: 37817781 DOI: 10.1016/j.sdentj.2023.05.019
    INTRODUCTION: Invasive surgical procedures in the oral cavity inevitably cause trauma to the soft and hard tissues. The healing process in the oral cavity tissue occurs in a complex manner involving different types of cells, maturation process, and the time of healing. Salvadora persica (miswak) has been found to exert various positive effects on the oral cavity, including antimicrobial, anti-gingivitis, anti-cariogenic, gingival healing, and teeth whitening properties. This study aims to investigate the potential of miswak as an adjunctive therapy in promoting wound healing.

    MATERIALS AND METHODS: 30 live Sprague-Dawley rats were used in this study. The rats' mandibular first molar tooth was extracted, and an incision wound was made on the tongue. The extraction socket and incision wound were irrigated using normal saline and different concentrations of locally processed miswak plant extracts (0.05%, 10%, and 20%) for 7 days. The rats were sacrificed for gross examination of the tooth socket and tongue healing. Both soft tissue and alveolar bone were examined microscopically.

    RESULTS: Complete closure of the incision wound was observed on all rats' tongues; miswak groups showed better wound healing than control and placebo groups in the oral mucosa overlying the alveolar bones. 0.05% and 20% miswak extracts showed prominent wound healing effects in the sagittal sections of the tongue, with moderate formation of connective tissue under the wound site and notable wound contraction. The 20% miswak extract group showed the highest percentage of healed oral mucosa on the alveolar bone and higher bone deposition at the alveolar base.

    CONCLUSION: A concentration of 20% miswak extract enhances the initial phase of wound healing both in oral soft and hard tissues. Miswak extract at this concentration was not toxic to the tissues and had potential therapeutic effects in oral tissue healing.

  7. Ab Malik N, Walls AWG
    Saudi Dent J, 2023 Sep;35(6):625-640.
    PMID: 37817782 DOI: 10.1016/j.sdentj.2023.06.004
    BACKGROUND: The number of older people increases globally, so is the risk of cognitive impairment. Periodontal diseases are common among older adults with significant tooth loss and periodontal problems. Thus, this review explored the periodontal disease conditions among individuals with and without dementia.

    METHODS: Available databases such as Medline/Pubmed, Web of Science, Scopus, Cochrane Library and Embase/OVID were used in the search. Case-control studies reporting on periodontal disease and dementia parameters were selected based on PICO (Population, Intervention, Comparison and Outcomes) framework. A Newcastle-Ottawa Scale (NOS) was used to assess the quality reporting of the studies and PRISMA guideline was used for screening.

    RESULTS: A total of ten studies were identified for analysis. Most studies reported higher plaque index score (PI), bleeding on probing (BoP), pocket depth (PD) and clinical attachment loss (CAL) among individuals diagnosed with dementia or Alzheimer's disease compared with clinically healthy controls or individual diagnosed without dementia. A higher prevalence of subjects with severe periodontal disease was also observed in individuals diagnosed with dementia/Alzheimer's disease. The quality of the studies was found to be moderate with lower comparability and ascertainment criteria scores.

    CONCLUSION: This qualitative analysis has shown poor periodontal health and increased inflammatory mediators in case groups compared to the control groups. Thus, more quality studies and novel intervention are warranted to reduce the impact of periodontal health on dementia globally.

  8. Joseph DK, Mat Ludin AF, Ibrahim FW, Ahmadazam A, Che Roos NA, Shahar S, et al.
    Front Physiol, 2023;14:1287170.
    PMID: 37817985 DOI: 10.3389/fphys.2023.1287170
    [This corrects the article DOI: 10.3389/fphys.2023.1216948.].
  9. Yow HY, Hamzah S, Abdul Rahim N, Suppiah V
    Asian Biomed (Res Rev News), 2023 Jun;17(3):95-114.
    PMID: 37818163 DOI: 10.2478/abm-2023-0050
    BACKGROUND: Statins are the most widely used lipid-lowering agents for patients with hyperlipidemia. However, interindividual variations in efficacy and risk of adverse drug reactions to statin treatment have been widely reported. Ethnicity is well known to be one of the contributing factors to this variation, particularly among Asians.

    OBJECTIVES: To identify genetic variants associated with statin treatment responses among Asian populations with a focus on four commonly prescribed statins: atorvastatin, rosuvastatin, simvastatin, and pravastatin.

    METHODS: A literature search was conducted in Medline and Embase databases. Studies published from 2008 to 2021 were included. The title and abstract of each article were screened by two reviewers and verified by another two reviewers. Data charted include information on authors, year of study, study population, statin studied, gene studied, study findings, and data of significant statistical value.

    RESULTS: A total of 35 articles were included from the 1,939 original studies related to treatment efficacy and 5 articles out of the 284 original studies related to adverse effects. Genetic variants in transmembrane transporters, cytochrome P450 isoenzymes, and apolipoproteins are the most extensively studied among Asian populations, with a main focus on ethnic Chinese. However, Asia consists of genetically different populations, and the results of this review indicated that there is a paucity of studies on other ethnic groups within Asia.

    CONCLUSIONS: Considering the ethnicity of patients could provide a potential value to personalized medicine in statin therapy.

  10. Akbar KA, Try P, Viwattanakulvanid P, Kallawicha K
    Saf Health Work, 2023 Sep;14(3):243-249.
    PMID: 37818214 DOI: 10.1016/j.shaw.2023.05.001
    BACKGROUND: Southeast Asia has many people who work in the agriculture sector. Not many stakeholders pay special attention to the health of farmers, even though they are exposed to various types of hazards. One of the most common health complaints among farmers is related to work-related musculoskeletal disorders (WMSDs). This study aims to assess the prevalence of WMSDs and factors associated with them among farmers in Southeast Asia.

    METHODS: A literature search on PubMed, ScienceDirect, Scopus, and EBSCO was conducted. Articles were included if they studied ergonomic problems among farmers from 11 countries in the Southeast Asia region and were published during 2015-2022. The Critical Appraisal Skills Program was used to evaluate the quality of the articles. The search process and retrieval process reflected PRISMA's recommendation.

    RESULT: There were 14 studies found from 3 countries that had published articles in PubMed and ScienceDirect, including Thailand [8], Indonesia [4], and Malaysia [2]. The prevalence of WMSDs in Thailand, Indonesia, and Malaysia was 78,31%, 81,27%, and 88,39%, respectively. Common factors associated with WMSDs include age, sex, smoking habits, drinking alcohol habits, working period (years), type of work, awkward position, non-ergonomic equipment, repetitive movements, and lifting heavy loads.

    CONCLUSION: The prevalence of WMSDs among farmers in Southeast Asia is considerably high. Effective intervention is essential for reducing the prevalence and protecting workers' health and well-being.

  11. Alsharqi M, Lapidaire W, Iturria-Medina Y, Xiong Z, Williamson W, Mohamed A, et al.
    Eur Heart J Imaging Methods Pract, 2023 Sep;1(2):qyad029.
    PMID: 37818310 DOI: 10.1093/ehjimp/qyad029
    AIMS: Accurate staging of hypertension-related cardiac changes, before the development of significant left ventricular hypertrophy, could help guide early prevention advice. We evaluated whether a novel semi-supervised machine learning approach could generate a clinically meaningful summary score of cardiac remodelling in hypertension.

    METHODS AND RESULTS: A contrastive trajectories inference approach was applied to data collected from three UK studies of young adults. Low-dimensional variance was identified in 66 echocardiography variables from participants with hypertension (systolic ≥160 mmHg) relative to a normotensive group (systolic < 120 mmHg) using a contrasted principal component analysis. A minimum spanning tree was constructed to derive a normalized score for each individual reflecting extent of cardiac remodelling between zero (health) and one (disease). Model stability and clinical interpretability were evaluated as well as modifiability in response to a 16-week exercise intervention. A total of 411 young adults (29 ± 6 years) were included in the analysis, and, after contrastive dimensionality reduction, 21 variables characterized >80% of data variance. Repeated scores for an individual in cross-validation were stable (root mean squared deviation = 0.1 ± 0.002) with good differentiation of normotensive and hypertensive individuals (area under the receiver operating characteristics 0.98). The derived score followed expected hypertension-related patterns in individual cardiac parameters at baseline and reduced after exercise, proportional to intervention compliance (P = 0.04) and improvement in ventilatory threshold (P = 0.01).

    CONCLUSION: A quantitative score that summarizes hypertension-related cardiac remodelling in young adults can be generated from a computational model. This score might allow more personalized early prevention advice, but further evaluation of clinical applicability is required.

  12. Abdul Manan MAF, Cordes DB, McKay AP, Mohammat MF, Mohd Aluwi MFF, Jumali NS
    IUCrdata, 2023 Sep;8(Pt 9):x230782.
    PMID: 37818467 DOI: 10.1107/S2414314623007824
    The title compound, C17H15N3OS2 was obtained from the condensation reaction of S-benzyl-dithio-carbazate and 5-methyl-isatin. In the solid-state, the mol-ecule adopts a Z configuration with the 5-methyl-isatin and di-thio-carbazate groups located on the same side of the C=N bond, involving an intra-molecular N-H⋯O hydrogen bond.
  13. Ramli NZ, Yahaya MF, Mohd Fahami NA, Abdul Manan H, Singh M, Damanhuri HA
    Front Aging Neurosci, 2023;15:1158001.
    PMID: 37818479 DOI: 10.3389/fnagi.2023.1158001
    The menopausal transition has been proposed to put women at risk for undesirable neurological symptoms, including cognitive decline. Previous studies suggest that alterations in the hormonal milieu modulate brain structures associated with cognitive function. This structured review provides an overview of the relevant studies that have utilized MRI to report volumetric differences in the brain following menopause, and its correlations with the evaluated cognitive functions. We performed an electronic literature search using Medline (Ovid) and Scopus to identify studies that assessed the influence of menopause on brain structure with MRI. Fourteen studies met the inclusion criteria. Brain volumetric differences have been reported most frequently in the frontal and temporal cortices as well as the hippocampus. These regions are important for higher cognitive tasks and memory. Additionally, the deficit in verbal and visuospatial memory in postmenopausal women has been associated with smaller regional brain volumes. Nevertheless, the limited number of eligible studies and cross-sectional study designs warrant further research to draw more robust conclusions.
  14. Niazi S, Eusufzai SZ, Saddki N
    Health Care Women Int, 2023;44(10-11):1466-1480.
    PMID: 35302903 DOI: 10.1080/07399332.2022.2046751
    We determined factors associate with oral health literacy of pregnant women. A total of 203 pregnant women attending Hospital Universiti Sains Malaysia (USM) for antenatal care participated in this cross-sectional study. The Malay version of Oral Health Literacy Instrument (OHLI-M) and a structured self-administered questionnaire were used to obtain variables of interest. The mean OHLI-M score was 73.6 (SD 12.59). Only slightly more than half of our participants had adequate oral health literacy (54.2%), while others were marginal (31.0%) and inadequate (14.8%). Our findings revealed that women with adequate oral health literacy were more likely to be those with higher education qualification and were very satisfied or satisfied with their oral health status. The identification of these predictors highlighted the importance to keep oral health education messages simple and clear for the benefit of individuals with low educational attainment.
  15. Herawati MH, Besral, Lolong DB, Pracoyo NE, Sukoco NEW, Supratikta H, et al.
    PLoS One, 2023;18(4):e0284162.
    PMID: 37104477 DOI: 10.1371/journal.pone.0284162
    One of the most important indicators in malaria eradication is the malaria surveillance information system (SISMAL) for recording and reporting medical cases. This paper aims to describe the availability and readiness of SISMALs at primary health centers (PHCs) in Indonesia. A cross-sectional survey was implemented in seven provinces for this study. The data was analyzed using bivariate, multivariate, and linear regression. The availability of the information system was measured by assessing the presence of the electronic malaria surveillance information system (E-SISMAL) at the studied PHCs. The readiness was measured by averaging each component of the assessment. From 400 PHC samples, only 58.5% had available SISMALs, and their level of readiness was only 50.2%. Three components had very low levels of readiness: (1) the availability of personnel (40.9%), (2) SISMAL integration and storage (50.2%), and (3) the availability of data sources and indicators (56.8%). Remote and border (DTPK) areas had a 4% better readiness score than non-DTPK areas. Endemic areas were 1.4% better than elimination areas, while regions with low financial capacity were 3.78% better than regions with high financial capacity, with moderate capacity (2.91%). The availability rate of the SISMAL at PHCs is only 58.5%. Many PHCs still do not have SISMALs. The readiness of the SISMAL at these PHCs is significantly related to DTPK/remote area, high endemicity status, and low financial capacity. This study found that the implementation of SISMAL is more accessible to malaria surveillance for the remote area and regions with low financial capacity. Therefore, this effort will well-fit to address barrier to malaria surveillance in developing countries.
    MeSH terms: Cross-Sectional Studies; Humans; Indonesia/epidemiology; Information Systems; Fitness Centers*
  16. Aboaoja FA, Zainal A, Ghaleb FA, Alghamdi NS, Saeed F, Alhuwayji H
    PeerJ Comput Sci, 2023;9:e1492.
    PMID: 37810364 DOI: 10.7717/peerj-cs.1492
    BACKGROUND: Malware, malicious software, is the major security concern of the digital realm. Conventional cyber-security solutions are challenged by sophisticated malicious behaviors. Currently, an overlap between malicious and legitimate behaviors causes more difficulties in characterizing those behaviors as malicious or legitimate activities. For instance, evasive malware often mimics legitimate behaviors, and evasion techniques are utilized by legitimate and malicious software.

    PROBLEM: Most of the existing solutions use the traditional term of frequency-inverse document frequency (TF-IDF) technique or its concept to represent malware behaviors. However, the traditional TF-IDF and the developed techniques represent the features, especially the shared ones, inaccurately because those techniques calculate a weight for each feature without considering its distribution in each class; instead, the generated weight is generated based on the distribution of the feature among all the documents. Such presumption can reduce the meaning of those features, and when those features are used to classify malware, they lead to a high false alarms.

    METHOD: This study proposes a Kullback-Liebler Divergence-based Term Frequency-Probability Class Distribution (KLD-based TF-PCD) algorithm to represent the extracted features based on the differences between the probability distributions of the terms in malware and benign classes. Unlike the existing solution, the proposed algorithm increases the weights of the important features by using the Kullback-Liebler Divergence tool to measure the differences between their probability distributions in malware and benign classes.

    RESULTS: The experimental results show that the proposed KLD-based TF-PCD algorithm achieved an accuracy of 0.972, the false positive rate of 0.037, and the F-measure of 0.978. Such results were significant compared to the related work studies. Thus, the proposed KLD-based TF-PCD algorithm contributes to improving the security of cyberspace.

    CONCLUSION: New meaningful characteristics have been added by the proposed algorithm to promote the learned knowledge of the classifiers, and thus increase their ability to classify malicious behaviors accurately.

  17. Nawaz R, Ullah H, Ghanim AAJ, Irfan M, Anjum M, Rahman S, et al.
    ACS Omega, 2023 Oct 03;8(39):36076-36087.
    PMID: 37810725 DOI: 10.1021/acsomega.3c04229
    ZnO and black TiO2 have been selected as the most efficient materials for organic pollution abatement due to their increased efficiency when compared to other materials. However, the concept of green chemistry makes it desirable to design green synthesis approaches for their production. In this study, black TiO2 was synthesized using an environmentally safe synthetic technique with glycerol as a reductant. ZnO was prepared by using ionic-liquid-based microwave-assisted extracts of Polygonum minus. To investigate the materials' potential to photodegrade organic pollutants, methylene blue (MB) and phenol were chosen as model organic pollutants. Both materials were found to exhibit spherical morphologies and a mesoporous structure and were efficient absorbers of visible light. ZnO exhibited electron-hole pair recombination lower than that of black TiO2. Black TiO2 was discovered to be an anatase phase, whereas ZnO was found to have a hexagonal wurtzite structure. In contrast to black TiO2, which had a surface area of 239.99 m2/g and a particle size of 28 nm, ZnO had a surface area of 353.11 m2/g and a particle size of 32 nm. With a degradation time of 60 min, ZnO was able to eliminate 97.50% of the 40 mg/L MB. Black TiO2, on the other hand, could reduce 90.0% of the same amount of MB in 60 min. When tested for phenol degradation, ZnO and black TiO2 activities were reduced by nearly 15 and 25%, respectively. A detailed examination of both ZnO and black TiO2 materials revealed that ZnO has more potential and versatility for the degradation of organic pollutants under visible light irradiation.
  18. Ngo TKN, Yang SJ, Mao BH, Nguyen TKM, Ng QD, Kuo YL, et al.
    Mater Today Bio, 2023 Dec;23:100820.
    PMID: 37810748 DOI: 10.1016/j.mtbio.2023.100820
    Metastasis is the leading cause of cancer-related deaths. During this process, cancer cells are likely to navigate discrete tissue-tissue interfaces, enabling them to infiltrate and spread throughout the body. Three-dimensional (3D) spheroid modeling is receiving more attention due to its strengths in studying the invasive behavior of metastatic cancer cells. While microscopy is a conventional approach for investigating 3D invasion, post-invasion image analysis, which is a time-consuming process, remains a significant challenge for researchers. In this study, we presented an image processing pipeline that utilized a deep learning (DL) solution, with an encoder-decoder architecture, to assess and characterize the invasion dynamics of tumor spheroids. The developed models, equipped with feature extraction and measurement capabilities, could be successfully utilized for the automated segmentation of the invasive protrusions as well as the core region of spheroids situated within interfacial microenvironments with distinct mechanochemical factors. Our findings suggest that a combination of the spheroid culture and DL-based image analysis enable identification of time-lapse migratory patterns for tumor spheroids above matrix-substrate interfaces, thus paving the foundation for delineating the mechanism of local invasion during cancer metastasis.
  19. Tan KY, Deng S, Tan TK, Hari R, Sitam FT, Othman RY, et al.
    PeerJ, 2023;11:e16002.
    PMID: 37810781 DOI: 10.7717/peerj.16002
    BACKGROUND: The Malayan pangolin (Manis javanica) is a placental mammal and is listed as Critically Endangered on the IUCN Red List of Threatened Species. Most previous attempts to breed pangolins in captivity have met with little success because of dietary issues, infections, and other complications, although a previous study reported breeding pangolins in captivity to the third generation. In our previous pangolin genome sequencing data analysis, we obtained a considerable amount of bacterial DNA from a pregnant female Malayan pangolin (named "UM3"), which was likely infected by Paraburkholderia fungorum-an agent of biodegradation and bioremediation in agriculture.

    METHODOLOGY: Here, we further confirmed and characterized this bacterial species using PCR, histological staining, whole-genome sequencing, and bioinformatics approaches. PCR assays with in-house designed primer sets and 16S universal primers showed clear positive bands in the cerebrum, cerebellum, lung, and blood of UM3 suggesting that UM3 might have developed septicaemia. Histological staining showed the presence of Gram-negative rod-shaped bacteria in the pangolin brain and lungs, indicating the colonization of the bacteria in these two organs. In addition, PCR screening of UM3's fetal tissues revealed the presence of P. fungorum in the gastrocnemius muscle, but not in other tissues that we examined. We also sequenced and reconstructed the genome of pangolin P. fungorum, which has a genome size of 7.7 Mbps.

    CONCLUSION: Our study is the first to present detailed evidence of the presence of P. fungorum in a pangolin and her fetus (although preliminary results were presented in our previous article). Here, we raise the concern that P. fungorum may potentially infect humans, especially YOPI (young, old, pregnant, and immunocompromised) people. Therefore, caution should be exercised when using this bacterial species as biodegradation or bioremediation agents in agriculture.

    MeSH terms: Animals; Female; Humans; Placenta; Pregnancy; Sequence Analysis
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