Role of extremophilic Bacillus cereus KH1 and its lipopeptide in treatment of organic pollutant in wastewater

Nor FHM 1 , Abdullah S 2 , Ibrahim Z 3 , Nor MHM 3 , Osman MI 4 , Al Farraj DA 5 Show all authors , AbdelGawwad MR 6 , Kamyab H 7

Affiliations 

  • 1 Department of Physics and Chemistry, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia, Pagoh Education Hub, Pagoh, 84600, Muar, Johor, Malaysia
  • 2 Department of Physics and Chemistry, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia, Pagoh Education Hub, Pagoh, 84600, Muar, Johor, Malaysia. shakilaa@uthm.edu.my
  • 3 Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia
  • 4 Setia Impian Development, Peringgit Centre, Taman Peringgit Jaya, 75400, Melaka, Malaysia
  • 5 Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh, 11451, Saudi Arabia
  • 6 Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, International University of Sarajevo, 71210, Sarajevo, Bosnia and Herzegovina
  • 7 Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100, Kuala Lumpur, Malaysia
Bioprocess Biosyst Eng, 2023 Mar;46(3):381-391.
PMID: 35779113 DOI: 10.1007/s00449-022-02749-1

Abstract

An effective biosurfactant producer and extremophiles bacteria, Bacillus cereus KH1, was isolated from textile effluent and the biosurfactant was produced using molasses as the sole carbon source. Growth parameters such as pH, temperature, salinity and concentration of molasses were optimised for decolourising the textile effluent with 24-h incubation. The biosurfactant property of B. cereus KH1 was evaluated based on haemolytic activity, oil displacement technique, drop-collapsing test and emulsification index. The results of the produced biosurfactant showed a positive reaction in haemolytic activity, oil displacement technique, drop-collapsing test and exhibiting a 67% emulsification index. The cell-free broth was stable in 40 °C pH 7, 7% salinity and 7% molasses. Thin-Layer Chromatography and Fourier Transform Infrared Spectroscopy analysis revealed that the biosurfactant was a lipopeptide with a yield 2.98 g L-1. These findings proved the synergistic action of B. cereus KH1 with lipopeptide biosurfactant may accelerated the decolourisation efficiency to 87%.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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