Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp

Silvanir 1 , Lai SY 2 , Asmawi AA 3 , Chew KW 4 , Ngan CL 5

Affiliations 

  • 1 School of Energy and Chemical Engineering, Xiamen University Malaysia, Sepang, Selangor Darul Ehsan 43900, Malaysia
  • 2 School of Energy and Chemical Engineering, Xiamen University Malaysia, Sepang, Selangor Darul Ehsan 43900, Malaysia; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China
  • 3 Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Gambang 26300, Pahang Darul Makmur, Malaysia
  • 4 School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, 637459, Singapore
  • 5 School of Energy and Chemical Engineering, Xiamen University Malaysia, Sepang, Selangor Darul Ehsan 43900, Malaysia; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China. Electronic address: clngan@xmu.edu.my
Bioresour Technol, 2024 Feb;393:130094.
PMID: 38000640 DOI: 10.1016/j.biortech.2023.130094

Abstract

Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist in protein release from microalgae. This study integrates HSM in liquid biphasic system with seven parameters being investigated on extraction efficiency (EE) and protein yield (Y). The highest EE and Y obtained are 96.83 ± 0.47 % and 40.98 ± 1.27 %, respectively, using 30% w/v K3PO4 salt, 60 % v/v alcohol, volume ratio of 1:1 and 0.5 % w/v biomass loading under shearing rate of 16,000 rpm for 1 min.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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