A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia

Goa Y 1 , Du JG 2 , Jirapattharasate C 3 , Galon E 4 , Ji SW 4 , Ran ZG 5 Show all authors , Xia YQ 1

Affiliations 

  • 1 The Key Sericultural Laboratory of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, 400715, PR China
  • 2 Department of Bacterial Biologics, China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Beijing 100-081, China
  • 3 Department of Pre-Clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, 999 Phutthamonthon Sai4, Salaya, Phutthamonthon, Nakhon Pathom, Thailand, 73170
  • 4 National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
  • 5 Chongqing Auleon Biological Co., Ltd., Rongchang, Chongqing, 402460, PR China
Trop Biomed, 2023 Dec 01;40(4):400-405.
PMID: 38308826 DOI: 10.47665/tb.40.4.004

Abstract

Beta toxin (CPB) is a lethal toxin and plays a key role in enterotoxemia of ruminants caused by Clostridium perfringens type C strain. The existing vaccines based on crude CPB need time-consuming detoxification and difficult quality control steps. In this study, we synthesized the rCPBm4 of C. perfringens type C strain and small ubiquitin-like modifier (SUMO)-tag CPBm4 (rSUMO-CPBm4) by introducing four amino acid substitutions: R212E, Y266A, L268G, and W275A. Compared with rCPBm4, rSUMO-CPBm4 was expressed with higher solubility in Escherichia coli BL21 (DE3). Neither rCPBm4 nor rSUMO-CPBm4 was lethal to mice. Although rCPBm4 and rSUMO-CPBm4 were reactogenic with polyclonal antibodies against crude CPB, rabbits vaccinated with rSUMO-CPBm4 developed significant levels of toxin-neutralizing antibody (TNA) titers that conferred protection against crude toxin challenge. These data suggest that genetically detoxified rSUMO-CPBm4 is a promising subunit vaccine candidate for C. perfringens type C beta enterotoxemia.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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