A two-step method of cyclolinopeptide (linusorb) preparation from flaxseed cake via dry-screening

Cai ZZ 1 , Xu CX 1 , Song ZL 1 , Li JL 1 , Zhang N 1 , Zhao JH 2 Show all authors , Lee YY 3 , Reaney MJT 4 , Huang FR 5 , Wang Y 6

Affiliations 

  • 1 Guangdong Saskatchewan Oilseed Joint Laboratory, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China
  • 2 Department of Materials Science and Engineering, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China
  • 3 School of Science, Monash University Malaysia, 47500 Bandar Sunway, Selangor, Malaysia
  • 4 Department of Plant Sciences, University of Saskatchewan, 51 Campus Dr., Saskatoon, Canada
  • 5 Department of Opto-Electronic Engineering, Jinan University, Guangzhou 510632, China. Electronic address: furong_huang@jnu.edu.cn
  • 6 Guangdong Saskatchewan Oilseed Joint Laboratory, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China. Electronic address: twyong@jnu.edu.cn
Food Chem, 2024 Aug 15;449:139243.
PMID: 38608605 DOI: 10.1016/j.foodchem.2024.139243

Abstract

Linusorbs (LO), cyclolinopeptides, are a group of cyclic hydrophobic peptides and considered a valuable by-product of flaxseed oil due to numerous health benefits. Currently applied acetone or methanol extraction could contaminate the feedstocks for further food-grade application. Using flaxseed cake as feedstock, this study established a practical method for preparing LO from pressed cake. Firstly, LO composition of 15 flaxseed cultivars was analyzed. Next, cold-pressed cake was milled and screened mechanically. The kernel and hull fractions were separated based on the disparity of their mechanical strength. Monitored by hyperspectral fluorescence, the LO-enriched kernel fraction separated from cold-pressed flaxseed cake was further used as feedstock for LO production. After ethanol extraction, partition, and precipitation, LOs were extracted from cold-pressed flaxseed cake with a purity of 91.4%. The proposed method could serve as feasible flaxseed cake valorization strategy and enable the preparation of other polar compounds such as flax lignan and mucilage.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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