Affiliations 

  • 1 Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, Selangor
Artif Cells Blood Substit Immobil Biotechnol, 1999 Sep-Nov;27(5-6):399-402.
PMID: 10595439

Abstract

Studies were carried out in T-flasks and bioreactor to produce urokinase enzyme using HT 1080 human kidney cell line. While growing the cell line it has been observed that the lag phase is reduced considerably in the bioreactor as compared to T-flask culture. The HT 1080 cell adhesion rate and urokinase production were observed to be the function of serum concentration in the medium. The maximum urokinase activity of 3.1 x 10(-4) unit ml(-1) was achieved in the bioreactor at around 65 h of batch culture. Since HT 1080 is an anchorage dependent cell line, therefore, the hydrodynamic effects on the cell line were investigated.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.