Needle Stick Injury is injuries caused by suture needle and hollow bore needle and does not include injuries via scalpel blades, lancet and glass pieces or by other means for the purpose of this training program. This problem needs to be prevented or minimized by embarking on an effective training program. The applicability of this program will be determined by the response of the participants to the contents and teaching methods which will in future effect the long term out come and the success of the Needle Stick Injury prevention and Universal Precautions training program. This Health Education program was designed to be used for the induction training of new workers as well as those already in service and is applicable to all levels of Health Care Workers especially those directly dealing with or handling syringes and needles. This Needle Stick Injury and Universal Precautions Training Program was carried out in one of the health facility under the Ministry of Health in Sabah and the outcome evaluation and analysis using paired t-test has shown a significant difference in their effectiveness in increasing the knowledge of the Health Care Workers after being exposed to the program (p < 0.01). The process evaluation was able to show that this approach was instrumental in creating awareness among the Health Care Workers who had attended this program. From the study, 98% (49) had not attended any formal training since joining the service, followed by 90% who had given a good overall rating for the program. For the lecture and its relevance, 90% and 74% for the hands–on training. About 77% of the participant was satisfied with the discussion. The program can be developed further and with it a comprehensive training module can be produced. The long-term benefits of such a training program will need to be evaluated in a future study.