Pinocembrin enriched fraction of Elytranthe parasitica (L.) Danser induces apoptosis in HCT 116 colorectal cancer cells

Kumar N 1 , Biswas S 2 , Hosur Shrungeswara A 3 , Basu Mallik S 2 , Hipolith Viji M 4 , Elizabeth Mathew J 5 Show all authors , Mathew J 6 , Nandakumar K 2 , Lobo R 7

Affiliations 

  • 1 Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India
  • 2 Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India
  • 3 Department of Pharmacy Practice, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India
  • 4 Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, University Sains Malaysia, Pulau Penang, Malaysia
  • 5 Department of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India
  • 6 Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India
  • 7 Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India. Electronic address: avemaria_lobo@yahoo.com
J Infect Chemother, 2017 Jun;23(6):354-359.
PMID: 28385566 DOI: 10.1016/j.jiac.2017.02.009

Abstract

BACKGROUND: Colorectal cancer (CRC) is a highly predominant malignancy affecting millions worldwide. Plants belonging to Loranthaceae family have remarkable chemopreventive properties.

OBJECTIVE: The goal of the present study was to assess the antiproliferative and apoptosis-inducing effects of stem parts of Elytranthe parasitica (L.) Danser (EP) on colorectal cancer and identify the bioactive phytochemicals.

MATERIAL AND METHODS: EP methanol extract (EP.M) and its subsequent fractions were screened for antiproliferative activity in human colorectal carcinoma HCT 116 cell line. Phytocomposition of the bioactive fraction was analyzed by GC-MS. Further, apoptotic induction and cell cycle arrest was assessed in the most bioactive fractions.

RESULTS: EP.DEE (Diethyl Ether) fraction and a subsequent fraction derived by column chromatography, Fraction 3A (FR 3A) significantly inhibited the proliferation of HCT 116 cells (P 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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