Structural characterization and evaluation of prebiotic activity of oil palm kernel cake mannanoligosaccharides

Kalidas NR 1 , Saminathan M 2 , Ismail IS 3 , Abas F 4 , Maity P 5 , Islam SS 6 Show all authors , Manshoor N 7 , Shaari K 8

Affiliations 

  • 1 Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: naveenareddy@ymail.com
  • 2 Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Animal Science, Faculty of Agriculture, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: saminathan@upm.edu.my
  • 3 Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: safinar@upm.edu.my
  • 4 Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: faridah_abas@upm.edu.my
  • 5 Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India. Electronic address: prasenjitchem89@gmail.com
  • 6 Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India. Electronic address: sirajul_1999@yahoo.com
  • 7 Faculty of Pharmacy, Universiti Teknologi Mara, 42300 Bandar Puncak Alam, Selangor, Malaysia. Electronic address: nurhuda15@puncakalam.uitm.edu.my
  • 8 Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: khozirah@yahoo.com.my
Food Chem, 2017 Nov 01;234:348-355.
PMID: 28551246 DOI: 10.1016/j.foodchem.2017.04.159

Abstract

In this study, mannanoligosaccharides (MOS) were isolated from palm kernel cake by aqueous extraction using high temperature and pressure. Structural characterization of MOS was carried out using acid hydrolysis, methylation analysis, ESI-MS/MS and 1D/2D NMR. The prebiotic activity of MOS was evaluated in vitro using two probiotic Lactobacillus strains. Sugar analysis indicated the presence of mannose in each of the oligomers. Methylation and 1D/2D NMR analysis indicated that the MOS have a linear structure consisting of (1→4)-β-d-mannopyranosyl residues. ESI-MS/MS results showed that the isolated mannan oligomers, MOS-III, MOS-IV, MOS-V and MOS-VI consist of tetra-, penta-, hexa-, and hepta-saccharides with molecular weights of 689, 851, 1013 and 1151Da, respectively. Based on the in vitro growth study, MOS-III and MOS-IV was found to be effective in selectively promoting the growth of Lactobacillus reuteri C1 strain as evidenced by the optical density of the culture broth.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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