Resistance Mechanism in a Terbinafine-Resistant Strain of Microsporum canis

Kano R 1 , Hsiao YH 2 , Siew HH 3 , Chen C 2 , Hasegawa A 4 , Kamata H 5

Affiliations 

  • 1 Department of Veterinary Pathobiology, Nihon University College of Bioresouce Sciences, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan. kanou.rui@nihon-u.ac.jp
  • 2 Asian Veterinary Specialist Referral Center, 1F, No. 109, Sec. 4, XingYi Rd., Taipei, 106, Taiwan
  • 3 , Johor Bahru, Malaysia
  • 4 Teikyo University Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo, 192-0395, Japan
  • 5 Department of Veterinary Pathobiology, Nihon University College of Bioresouce Sciences, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan
Mycopathologia, 2018 Jan 16.
PMID: 29340910 DOI: 10.1007/s11046-018-0242-0

Abstract

To clarify the terbinafine (TRF) resistance mechanism in a TRF-resistant strain of Microsporum canis, the expression of the pleiotropic drug resistance (PDR1), multidrug resistance (MDR1), MDR2 and MDR4 genes were investigated by real-time quantitative PCR (RT-qPCR) analysis, given the known interaction of the corresponding proteins with antifungals and with the efflux blocker FK506. The expression of the PDR1, MDR1, MDR2 and MDR4 genes was 2-4 times higher in the TRF-resistant strain grown in the presence of 0.14 µg/mL of TRF than in TRF-susceptible strains cultured in the absence of TRF. The TRF-resistant strain exhibited MICs of > 32 µg/mL for TRF alone; this resistance was attenuated to an MIC of 8 µg/mL in the presence of FK506, indicating that the TRF inhibitory concentration index value was

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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