• 1 General Surgery Department, The People's Hospital of Jianhu, Yancheng, 224700, China
  • 2 General Surgery Department, Chang'an Hospital, Xi'an, 710016, China
  • 3 Department of General Surgery, Ankang People's Hospital, Ankang, 725000, China
  • 4 Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Darul Ehsan 43400, Malaysia
  • 5 Department of Botany, Gandhi Faiz-e-Aam College, Shahajahanpur 242001, Uttar Pradesh, India
  • 6 Department of Chemistry, Faculty of Science and Arts, and Promising Center for Sensors and Electronic Devices (PCSED), Najran University, Najran 11001, Kingdom of Saudi Arabia
J Nanosci Nanotechnol, 2018 May 01;18(5):3673-3681.
PMID: 29442882 DOI: 10.1166/jnn.2018.15364


Nanobiotechnology has emerged as a promising technology to develop new therapeutically active nanomaterials. The present study was aimed to biosynthesize AgNPs extracellularly using Aspergillus niger JX556221 fungal extract and to evaluate their anticancer potential against colon cancer cell line, HT-29. UV-visible spectral characterization of the synthesized AgNPs showed higher absorption peak at 440 nm wavelength. Transmission Electron Microscopy (TEM) analysis revealed the monodispersed nature of synthesized AgNPs occurring in spherical shape with a size in the range of 20-25 nm. Further, characterization using Energy Dispersive Spectroscopy (EDX) confirmed the face-centred cubic crystalline structure of metallic AgNPs. FTIR data revealed the occurrence of various phytochemicals in the cell free fungal extract which substantiated the fungal extract mediated AgNPs synthesis. The cytotoxic effect of AgNPs was studied by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results evidenced the cytotoxic effect of AgNPs on HT-29 cell lines in a dose dependent manner. The highest activity was found at 100 μg/ml concentration after 24 h of incubation. Use of propidium iodide staining examination method confirmed the cytotoxic effect of AgNPs through inducing cell apoptosis. AgNPs cytotoxicity was found to be through elevating reactive oxygen species (ROS), and caspase-3 activation resulting in induced apoptosis. Therefore, this research finding provides an insight towards the development of novel anticancer agents using biological sources.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.