A safety evaluation test on human for latex films made from Radiation Prevulcanized Natural Rubber Latex (RVNRL) with sulfur-containing antioxidant was studied. Sulfur test has confirmed that there was sulfur compound presence in RVNRL derived from antioxidant used in this study. Two types of safety evaluation test were being adopted which are Patch Test and Modified Draize-95 test and this test proved that there is no clinical evidence on the presence of sulfur compound in RVNRL that may induce Type IV allergy in the unsensitized general user population. Both clinical test shows that the highest score value produced by test subjects is 1 and not exceed the allowable limit.