Despite the continued effort globally made to control the growing case of Tuberculosis (TB), it
continues to be regarded as the second deadliest disease after the HIV. There are various
methods developed to diagnose TB, most of which having the criteria of sensitive, selective,
cheap and portable to be used in robust applications. Even with the advancement in medication,
the important keys including early stage diagnosis is yet to be considered. In diagnosing TB, the
only technique remained as the gold standard method is the culturing method, which is the Acid
Fast Bacilli (AFB) staining. On the other hand, molecular technique utilising Polymerase Chain
Reaction (PCR) assay is preferred as a non-culturing method. Additionally, as molecular
techniques become advanced, real-time PCR or quantitative PCR (qPCR) using multiple probes
in one shot has raised interest among researchers, because it can skip the process of gel
electrophoresis. Recently, researchers have been working on electrochemical DNA sensors
which are sensitive, selective, rapid, cheap and can meet with point of care (POC) testing
requirements to diagnose TB.