Displaying publications 1 - 20 of 208 in total

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  1. The staining of combined thick and thin blood films on the same slide with modified Field's rapid stain
    Coombs GL, Sandosham AA
    Med J Malaya, 1965 Sep;20(1):53.
    PMID: 4158839
    Matched MeSH terms: Staining and Labeling*
  2. The cell types found in the pharynx of Polystomoides malayi Rohde
    Rohde K
    Med J Malaya, 1965 Sep;20(1):55.
    PMID: 4158840
    Matched MeSH terms: Staining and Labeling*
  3. Studies on Echinostomatidae (Trematoda) in Malaya. XIV. Body gland cells in cercariae of Echinostoma audyi Lie and Umathevy, and E. lindoense Sandground and Bonne
    Lie KJ
    J Parasitol, 1966 Dec;52(6):1049-51.
    PMID: 4162736
    Matched MeSH terms: Staining and Labeling
  4. Morphological characteristics of developmental stages of Acanthamoeba and Naegleria species before and after staining by various techniques
    Ithoi I, Ahmad AF, Mak JW, Nissapatorn V, Lau YL, Mahmud R
    Southeast Asian J. Trop. Med. Public Health, 2011 Nov;42(6):1327-38.
    PMID: 22299400
    Seven stains were studied to determine the best color and contrast for staining the developmental stages of free living pathogenic Acanthamoeba and Naegleria species. The acid-fast bacilli stain (AFB) produced a blue color without contrast; trichrome-eosin and modified Field's showed various color contrasts; Giemsa, iron-hematoxylin, modified AFB and Gram produced only one color which distinguished the nucleus, nucleolus, cytoplasm, food- and water-vacuoles. The motile organs (acanthopodia, pseudopodia, lobopodia and flagella) were also clearly differentiated but produced a similar color as the cytoplasm. These motile organelles were first induced by incubating at 37 degrees C for at least 15 minutes and then fixing with methanol in order to preserve the protruding morphology prior to staining. The trichrome-eosin and iron-hematoxylin stains showed good color contrast for detecting all three stages, the trophozoite, cyst and flagellate; Giemsa and Gram stained the trophozoite and flagellate stages; the modified Field's and modified AFB stains stained only the trophozoite stage. Depending on the purpose, all these stains (except the AFB stain) can be used to identify the developmental stages of Acanthamoeba and Naegleria for clinical, epidemiological or public health use.
    Matched MeSH terms: Staining and Labeling/methods*
  5. NOTES ON THE TRANSFER OF MALARIA PARASITES DURING MASS STAINING OF THICK BLOOD FILMS
    HOO CC
    Med J Malaysia, 1963 Dec;18:129-30.
    PMID: 14117282
    Matched MeSH terms: Staining and Labeling*
  6. Fulltext Evaluation of in vitro osteoblast and osteoclast differentiation from stem cell: a systematic review of morphological assays and staining techniques
    Zainal Ariffin SH, Megat Abdul Wahab R, Abdul Razak M, Yazid MD, Shahidan MA, Miskon A, et al.
    PeerJ, 2024;12:e17790.
    PMID: 39071131 DOI: 10.7717/peerj.17790
    BACKGROUND: Understanding human stem cell differentiation into osteoblasts and osteoclasts is crucial for bone regeneration and disease modeling. Numerous morphological techniques have been employed to assess this differentiation, but a comprehensive review of their application and effectiveness is lacking.

    METHODS: Guided by the PRISMA framework, we conducted a rigorous search through the PubMed, Web of Science and Scopus databases, analyzing 254 articles. Each article was scrutinized against pre-defined inclusion criteria, yielding a refined selection of 14 studies worthy of in-depth analysis.

    RESULTS: The trends in using morphological approaches were identified for analyzing osteoblast and osteoclast differentiation. The three most used techniques for osteoblasts were Alizarin Red S (mineralization; six articles), von Kossa (mineralization; three articles) and alkaline phosphatase (ALP; two articles) followed by one article on Giemsa staining (cell morphology) and finally immunochemistry (three articles involved Vinculin, F-actin and Col1 biomarkers). For osteoclasts, tartrate-resistant acid phosphatase (TRAP staining) has the highest number of articles (six articles), followed by two articles on DAPI staining (cell morphology), and immunochemistry (two articles with VNR, Cathepsin K and TROP2. The study involved four stem cell types: peripheral blood monocyte, mesenchymal, dental pulp, and periodontal ligament.

    CONCLUSION: This review offers a valuable resource for researchers, with Alizarin Red S and TRAP staining being the most utilized morphological procedures for osteoblasts and osteoclasts, respectively. This understanding provides a foundation for future research in this rapidly changing field.

    Matched MeSH terms: Staining and Labeling/methods
  7. Studies on Echinostomatidae (Trematoda) in Malaya. 13. Integumentary papillae on six species of Echinostome cercariae
    Lie KJ
    J Parasitol, 1966 Dec;52(6):1041-8.
    PMID: 4162735
    Matched MeSH terms: Staining and Labeling
  8. Fulltext Comparison of calcofluor white M2R fluorescence and modified gram chromotrope kinyoun staining methods for the detection of microsporidial spores from stool samples
    Nur Raihana, I., Malina, O., Fatmah, M.S., Norhayati, M., Eni Juraida, A.R., Hama, R.A.
    Malaysian Journal of Medicine and Health Sciences, 2013;9(2):63-68.
    MyJurnal
    Routine diagnosis of intestinal microsporidiosis in clinical diagnostic laboratories relies mostly on detection of microsporidial spores via special staining and microscopic techniques. This paper describes the comparative evaluation of Calcofluor White M2R method, with modified Gram-chromotrope Kinyoun method as the reference standard. One hundred and six stool samples were examined for the presence of microsporidial spores. Sensitivity, specificity, positive and negative predictive values of the Calcofluor White M2R method compared to the reference technique were 95.2%, 4.3%, 78.2% and 20.0%, respectively. The positive predictive value (PPV) was 78.2% and the negative predictive value (NPV) was 20.0%. Despite low specificity of the CFW method due to its ability to stain chitinous wall of microorganisms, the presence of distinct deep-blue horizontal or equitorial stripes in microsporidial spores in modified Gram-chromotrope Kinyoun would likely reduce the false positive results obtained in the Calcofluor White M2R. Hence, the simultaneous use of these two methods would give better performance and accuracy for the detection of microsporidial spores in patients with intestinal microsporidiosis.
    Matched MeSH terms: Staining and Labeling
  9. Fulltext Histological Studies of Dentine Using Van Gieson And Gomori Trichome Dyes
    Mohd. Bakri, M, Mat Salleh, A.
    Ann Dent, 2003;10(1):-.
    MyJurnal
    Decalcified permanent teeth were sectioned and stained with Van Gieson and Gomori trichome dyes. Sections dyed with the Van Gieson dye did not produce any zones in dentine but with the Gomori trichome dye, four different zones of dentine were produced. Zone 1 begins at the predentine-dentine junction while zone 4 ends at the enamel - dentine junction. In zone 1, the intertubular dentine was stained clearly while in zone 3 intense staining of the peritubular dentine was observed. The result of this study supports the previous findings reported by other workers that the formation of intertubular dentine takes place in zone 1 while peritubular dentine occurs in zone 3.
    Matched MeSH terms: Staining and Labeling
  10. ABO grouping studies of human seminal stains on fabrics
    Sinnapa S, Soon LS
    Med J Malaya, 1970 Jun;24(4):278-86.
    PMID: 4096943
    Matched MeSH terms: Staining and Labeling
  11. Fulltext Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride
    Lee HY, Chai LC, Pui CF, Mustafa S, Cheah YK, Nishibuchi M, et al.
    Braz J Microbiol, 2013;44(1):51-5.
    PMID: 24159283 DOI: 10.1590/S1517-83822013005000004
    Biofilm formation can lead to various consequences in the food processing line such as contamination and equipment breakdowns. Since formation of biofilm can occur in various conditions; this study was carried out using L. monocytogenes ATCC 19112 and its biofilm formation ability tested under various concentrations of sodium chloride and temperatures. Cultures of L. monocytogenes ATCC 19112 were placed in 96-well microtitre plate containing concentration of sodium chloride from 1-10% (w/v) and incubated at different temperature of 4 °C, 30 °C and 45 °C for up to 60 h. Absorbance reading of crystal violet staining showed the density of biofilm formed in the 96-well microtitre plates was significantly higher when incubated in 4 °C. The formation of biofilm also occurs at a faster rate at 4 °C and higher optical density (OD 570 nm) was observed at 45 °C. This shows that storage under formation of biofilm that may lead to a higher contamination along the processing line in the food industry. Formation of biofilm was found to be more dependent on temperature compared to sodium chloride stress.
    Matched MeSH terms: Staining and Labeling
  12. Single incubation double esterase cytochemical reaction using a single coupling reagent
    Ainoon O, Jabamoney AJ, Cheong SK
    Malays J Pathol, 1991 Jun;13(1):47-9.
    PMID: 1724544
    Most methods used in double esterase cytochemistry for the diagnosis and classification of acute myeloid leukaemias require double incubation and staining, using separate coupling reagents. We evaluated a method by Swirsky on our normal and abnormal blood and bone marrow smears where only a single incubation and the use of a single coupling reagent is required. Its short incubation period and its strong positive reaction for butyrate esterase in demonstrating cells of monocytic lineage gives it an advantage over the conventional double incubation technique.
    Matched MeSH terms: Staining and Labeling
  13. Fulltext Influence of exposure time to coffee on color stability of selected composite resin veneers
    Thiyab, Amar M., Mahmood, Nik Zarina N., Hassan, Mohamed Ibrahim A.
    Compendium of Oral Science, 2016;3(1):9-16.
    MyJurnal
    Objectives: The aim of the study is to evaluate the effect of the time and instant coffee solution on the color stability of three types of composite resin based veneer systems. Materials and Methods: 24 composite resin veneer samples were selected and divided into three groups: two groups of prefabricated veneers (Edelweiss, Ultradent Inc™ (EDL) and Componeer, Coltène/ Whaledent AGTM (CMP)) and one group of laboratory made (Nexco, Ivoclar Vivadent (NEX)) veneer system were tested (n=8). Specimens were prepared and stored in staining solution (instant coffee) and assessed color changes with Minolta spectrophotometer every three days for a period of 27 days, after which color differences (ΔE*) were calculated. Data collection and analysis was done using one-way ANOVA and Student’s t-test (α=0.05). Results: One-way ANOVA revealed a significant difference in color stability between the two veneer systems. NEX group veneer system exhibited the highest color stability (ΔE*= 0.73 ± 0.5) as compared to prefabricated veneer groups (EDL 10.07 ± 5.15, CMP 7.41 ± 4.64) with p value
    Matched MeSH terms: Staining and Labeling
  14. Fulltext Comparative study on cartilage tissue collected from less- and severely-affected region of osteoarthritic knee
    Nurzazlin, B.Z.N., Shamsul, B.S., Yahya, N.H.M., Ruszymah, B.H.I., Abdul Rani, R., Chowdhury, S.R.
    Medicine & Health, 2018;13(1):77-87.
    MyJurnal
    Culture expanded chondrocytes isolated from non-load bearing region of osteoarthritic (OA) joint has been used to construct tissue engineered cartilage for treatment purposes. The aim of the study was to compare the histological properties of the cartilage tissue and morphological properties of the chondrocytes isolated from less and severely affected OA knee. Human articular cartilage was obtained as redundant tissue from consented patients with late-stage OA undergoing total knee replacement surgery at Universiti Kebangsaan Malaysia Medical Centre (UKMMC). Articular cartilage was graded according to Dougados and Osteoarthritis Research Society International (OARSI) classification. Articular cartilage was classified into less affected (LA; Grade 0-1) and severely affected (SA; Grade 2-3). Cartilage tissue from less and severely affected region was stained with Safranin O staining. Isolated chondrocytes from each group were cultured until passage 4 (P4). Their growth patterns, cell areas, and circularity were compared. LA-cartilage tissue shows uniform spread of safranin O staining indicating intact extracellular matrix (ECM) component. However, SA-cartilage shows significant reduction and unstable staining due to its degraded ECM. LA-chondrocytes showed an aggregated growth compared to SA-chondrocyte that remains monolayer. Moreover, LA-chondrocytes have significantly higher cell area with wider spreading at passage 0 and 4 compared to SA-chondrocytes. It was also found that chondrocyte circularity increased with passage, and circularity of LAchondrocytes was significantly higher than that of the SA-chondrocytes at passage 3. This study demonstrated the considerable difference in the cellular properties for less and severely affected chondrocytes and implication of these differences in cell-based therapy needed to be explored.
    Matched MeSH terms: Staining and Labeling
  15. Fulltext The effect of daily religious fasting on tear film characteristics and ocular surface
    Wan Nur Farhana Ibrahim, Mohd Zulfaezal Che Azemin
    International Journal of Allied Health Sciences, 2018;2(1):108-116.
    MyJurnal
    Religious fasting is an act of refraining oneself from eating and drinking beginning at dawn until sunset. The changes in meal time and long period of meal constraint may influence the tear quality and ocular surface. The purpose of this study was to investigate the effect of daily religious fasting on tear film characteristics and ocular surface integrity. This is a prospective study involving 29 eyes from 29 healthy participants. The tear film characteristics were assessed by measuring the non-invasive tear break-up time (NITBUT), tear meniscus height (TMH), total tear secretion, and
    fluorescein ocular surface staining method was used to determine the ocular surface integrity. The measurements were performed in the morning (8.00 to 10.00 a.m.) and evening (4.00 to 6.00 p.m.) during each non-fasting and fasting period. The results showed no statistically significant difference noted for all parameters measured in the morning when comparison was made between non-fasting and fasting periods. Conversely, in the evening, NITBUT value was significantly lower during fasting period, (p = 0.001), but, TMH, total tear secretion and ocular surface staining revealed no significant differences between non-fasting and fasting periods. Our study revealed
    that daily religious fasting only significantly reduced the NITBUT value in the evening which possibly due to dehydration; however, it did not affect TMH, total tear secretion and ocular surface integrity. The absence of fluid loading at pre-dawn meal could be the reason of non-noticeable differences noted in the morning
    Matched MeSH terms: Staining and Labeling
  16. Ability of whitening toothpastes in removing stains from composite resins
    Chong SY, Lim TB, Seow LL
    Malaysian Dental Journal, 2008;29(2):97-103.
    MyJurnal
    Objectives: The objectives of the study were to assess: i) the staining susceptibility of composite resins, ii) the ability of whitening toothpastes in removing stains from composite resins.
    Materials and Methods: Thirty specimens from each composite resins: Filtek Z350 (3M ESPE), Filtek Z250 (3M ESPE) and Beautifil (Shofu Inc.) were fabricated. After polishing, specimens were immersed in coffee for 3 days. Specimens were then brushed twice a day for 2 weeks using Colgate Total (Colgate-Palmolive, control group), Colgate Advanced Whitening (Colgate- Palmolive, test group) and Darlie All Shiny White (Hawley & Hazel Chemical Co., test group). Colour changes (?E*) were measured using Spectrophotometer at baseline, after coffee immersion and after brushing. Results were statistically analyzed using one way ANOVA and Tukey’s test.
    Results: There was significant difference in terms of colour changes for Filtek Z350, Filtek Z250 and Beautifil after coffee immersion (P0.05).
    Conclusions: Filtek Z350 was able to resist staining by coffee better than Filtek Z250 and Beautifil. The whitening toothpastes did not offer added advantage in terms of ability to remove stains compared to ordinary toothpaste.
    Matched MeSH terms: Staining and Labeling
  17. [3 Malayan trematodes]
    Rohde K, Lee SK, Lim HW
    Ann Parasitol Hum Comp, 1968 Jan-Feb;43(1):33-43.
    PMID: 4192885
    Matched MeSH terms: Staining and Labeling
  18. Fulltext Silver Colloid Staining of Sclerotic and Non-sclerotic Dentine
    Mohd. Bakri, M., Mohamed, N.H., Whittaker, D.A.
    Ann Dent, 2003;10(1):-.
    MyJurnal
    Phosphophoryn, a higWyphosphorylated protein, is the most abundant protein among the non-collagenous protein of dentine. The staining of phosphophoryn can be done by using the silver colloid staining. In this paper, the staining effect of the silver colloid stain on both non-sclerotic and sclerotic dentine was investigated. Eight teeth from donors aged 14, 17, 22, 34, 55, 57, 60 and 65 were used for this experiment. The younger teeth were used to demonstrate normal root dentine while the older age teeth were used to demonstrate sclerotic root dentine at the apical region of the root. There was no staining of the normal root dentine as compared to sclerotic dentine when the silver colloid staining was used.
    Matched MeSH terms: Staining and Labeling
  19. Fulltext Determination of time of death based on basic histological stain and immunostain changes
    Ezlan Elias, Khairul Osman, Sharifa Abdul Aziz, Abdul Halim Mansar, Siti Fatimah Ibrahim, Jamaludin Mohamed
    Jurnal Sains Kesihatan Malaysia, 2004;2(2):63-70.
    MyJurnal
    Establishing time of death has been extensively studied for the last 30 years. Parameters that have been studied included body temperature, biochemistry of rigor mortis, putrefactive changes and entomology. Despite an extensive study in these parameters it was found that all of the parameters were very much dependent on external factors like changes in surrounding temperature and activities done prior to death. To solve this problem, we decided to monitor the mechanism that occurs during death. Until now, various researches have found that during the early stage of death, heart and perfusion to the cells will stop. This will cause the cells to start the death process. The death of the cell will occurs either through apoptosis or necrosis. During apoptosis the cells will switch on and off a few proteins in a sequence. Based on this understanding, a study was conducted to determine if area ratio of apoptosis: necrosis and apoptotic p53 and Bcl-2 markers can be used as a reliable postmortem interval marker (PMI). Sampling of the study had involved 100 dead human skins with a known PMI. All samples were obtained from forensic unit of Hospital Kuala Lumpur (UFHKL). Ratio of apoptosis: necrosis areas were determined using hematoxilin and eosin staining while apoptosis p53 and Bcl-2 markers were done using an apoptosis kit. All staining were then indexed and plotted against PMI data obtained from UFHKL. Results indicated that there were no significant correlations between ratio of apoptosis: necrosis area against PMI (p = 0.144). Whereas for both apoptotic markers p53 and Bcl-2 PMI had shown a significant correlation (p < 0.000 for both results). In conclusion, we suggest that p53 and Bcl-2 parameters should be studied further since it is very likely that it could be a good indicator for PMI.
    Matched MeSH terms: Staining and Labeling
  20. Fulltext A rare case of recurrent orbital solitary fibrous tumour with intracranial extension
    Ainal Adlin N, Umi Kalthum MN, Amizatul Aini S, Reena Rahayu MZ
    Journal of Surgical Academia, 2016;6(1):59-61.
    MyJurnal
    A 47-year-old lady, presented with progressive proptosis of left eye with deterioration of vision. She had a history of left solitary fibrous tumour and had undergone left frontal craniotomy and orbitotomy in 2004. Surveillance Magnetic resonance imaging (MRI) six years later showed tumour recurrence with intracranial extension. However, she did not follow-up and only presented again 3 years, later. Tumour resection and left exenteration was performed. Histology showed ‘patternless’ pattern of neoplastic cells, and CD34 staining was diffusely positive. Diagnosis of recurrent solitary fibrous tumour with intracranial extension was made.
    Matched MeSH terms: Staining and Labeling
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