Bioethanol is one of the leading alternative biofuel to fossil fuels and can be use in
existing gasoline engines without any major modification. Bioethanol can be
produced from sugar containing biomass fermentation using different potential
strains. In this study, the gram negative, facultative anaerobic, rod shaped strain
‘Zymomonas mobilis’ was used as microorganism to produce bioethanol from sugar
cane molasses using anaerobic fermentation. The study was conducted to investigate
the optimized conditions for production of bioethanol through batch fermentation
process. The fermentation unit was designed to determine the effect of process
parameters such as fermentation temperature, pH, sugar concentration and supply of
nutrients. The Zymomonas mobilis produced 9.3% (v/v) bioethanol by utilizing 16
g/100mL sugar with the fermentation efficiency 92.5%. The fertilized based nutrients
were supplied to enhance the production of bioethanol yield. The bioethanol yield
produced by using this strain in optimized conditions is in good compromise with
previous study and also compared with commercially available yeast strain.