Production, structure and morphology of exopolysaccharides yielded by submerged fermentation of Antrodia cinnamomea

Chen L 1 , Wang Z 2 , Zhang B 3 , Ge M 4 , Ng H 5 , Niu Y 6 Show all authors , Liu L 7

Affiliations 

  • 1 Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: leichen@jiangnan.edu.cn
  • 2 Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: wzq7606@126.com
  • 3 Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: bobozhang@jiangnan.edu.cn
  • 4 Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: supermdge@163.com
  • 5 Faculty of Applied Sciences, UCSI University, UCSI Heights, Cheras, 56000 Kuala Lumpur, Malaysia. Electronic address: GrraceNg@ucsiuniversity.edu.my
  • 6 Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: yugeniu@sjtu.edu.cn
  • 7 State Key Laboratory of Food Science and Technology, Jiangnan University, WuXi 214122, China. Electronic address: mingll@jiangnan.edu.cn
Carbohydr Polym, 2019 Feb 01;205:271-278.
PMID: 30446105 DOI: 10.1016/j.carbpol.2018.10.070

Abstract

Carbon and nitrogen sources in culture medium of Antrodia cinnamomea were optimized to eliminate the interference of exterior macromolecules on exopolysaccharide (EPS) yield by submerged fermentation. The results suggested that culture medium containing 50 g/L of glucose and 20 g/L of yeast extract as the optimal carbon and nitrogen sources could produce 1.03 g/L of exopolysaccharides. After purification, two heteropolysaccharides (AC-EPS1 and AC-EPS2) were obtained and characterized to provide the basic structure information. As the main component of the produced EPS, AC-EPS2 (accounting for 89.63%) was mainly composed of galactose (87.42%) with Mw (molecular weight) and R.M.S. (root-mean-square) radius of 1.18 × 105 g/mol and 25.3 nm, respectively. Furthermore, the spherical and flexible chain morphologies of EPS were observed in different solvents by TEM. The structural and morphological information of purified EPS were significant for further study on their structure-activity relationship and related applications.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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